Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae

Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the e...

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Autores principales: Alemayehu Dugassa, Tesfaye Alemu, Yitbarek Woldehawariat
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Lenguaje:EN
Publicado: SpringerOpen 2021
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spelling oai:doaj.org-article:2c310c2daa434b1eba34643cbd5d95d62021-11-07T12:07:30ZScreening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae10.1186/s41938-021-00483-62536-9342https://doaj.org/article/2c310c2daa434b1eba34643cbd5d95d62021-11-01T00:00:00Zhttps://doi.org/10.1186/s41938-021-00483-6https://doaj.org/toc/2536-9342Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.Alemayehu DugassaTesfaye AlemuYitbarek WoldehawariatSpringerOpenarticleBiocontrolCo-inoculationGall diseaseIn-vivoRhizobacteriaVicia fabaAgricultureSENEgyptian Journal of Biological Pest Control, Vol 31, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biocontrol
Co-inoculation
Gall disease
In-vivo
Rhizobacteria
Vicia faba
Agriculture
S
spellingShingle Biocontrol
Co-inoculation
Gall disease
In-vivo
Rhizobacteria
Vicia faba
Agriculture
S
Alemayehu Dugassa
Tesfaye Alemu
Yitbarek Woldehawariat
Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
description Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.
format article
author Alemayehu Dugassa
Tesfaye Alemu
Yitbarek Woldehawariat
author_facet Alemayehu Dugassa
Tesfaye Alemu
Yitbarek Woldehawariat
author_sort Alemayehu Dugassa
title Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
title_short Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
title_full Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
title_fullStr Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
title_full_unstemmed Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae
title_sort screening of indigenous rhizobacteria as potential biological control against faba bean (vicia faba l.) gall disease caused by olpidium viciae
publisher SpringerOpen
publishDate 2021
url https://doaj.org/article/2c310c2daa434b1eba34643cbd5d95d6
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