Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.

Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+...

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Autores principales:	Chris Juul Hedegaard, Christian Enevold, Finn Sellebjerg, Klaus Bendtzen, Claus Henrik Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c36a59a22a64d47bd110bbaee8755ae
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spelling	oai:doaj.org-article:2c36a59a22a64d47bd110bbaee8755ae2021-11-18T06:53:35ZVariation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.1932-620310.1371/journal.pone.0020253https://doaj.org/article/2c36a59a22a64d47bd110bbaee8755ae2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625457/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p<0.04). Interleukin (IL)-5 was induced by MBP in MNC from the same five carriers versus two (9%) homozygotes (p<0.004); four carriers (57%) versus three non-carriers (14%) exhibited IL-17 responses to MBP (p<0.04). By contrast, we found no association between the polymorphisms investigated and interferon-gamma-, tumor necrosis factor-alpha-, IL-2, -4- or IL-10 responses to MBP. These results indicate that the rs5743291 polymorphism influences T helper (Th) cell 2- and Th17 cell responses in MNC from MS patients.Chris Juul HedegaardChristian EnevoldFinn SellebjergKlaus BendtzenClaus Henrik NielsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20253 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chris Juul Hedegaard Christian Enevold Finn Sellebjerg Klaus Bendtzen Claus Henrik Nielsen Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
description	Variations in the gene for the nucleotide-binding oligomerisation domain (NOD) 2 have been associated with Crohn's disease but not multiple sclerosis (MS). Here we investigate the effect of three polymorphisms in the NOD2 gene (rs5743277, rs2066842 and rs5743291) on cytokine production and CD4+ T cell proliferation elicited by human myelin basic protein (MBP) in blood mononuclear cell (MNC) cultures from 29 patients with MS. No polymorphism was observed at rs5743277. No associations with the rs2066842 polymorphism were found. Concerning rs5743291, none were homozygous for the minor allele. Seven of 29 (24%) patients were heterozygous, and five of these (71%) exhibited increased MBP-induced CD4+ T cell proliferation versus four of 22 (18%), who were homozygous for the major allele (p<0.04). Interleukin (IL)-5 was induced by MBP in MNC from the same five carriers versus two (9%) homozygotes (p<0.004); four carriers (57%) versus three non-carriers (14%) exhibited IL-17 responses to MBP (p<0.04). By contrast, we found no association between the polymorphisms investigated and interferon-gamma-, tumor necrosis factor-alpha-, IL-2, -4- or IL-10 responses to MBP. These results indicate that the rs5743291 polymorphism influences T helper (Th) cell 2- and Th17 cell responses in MNC from MS patients.
format	article
author	Chris Juul Hedegaard Christian Enevold Finn Sellebjerg Klaus Bendtzen Claus Henrik Nielsen
author_facet	Chris Juul Hedegaard Christian Enevold Finn Sellebjerg Klaus Bendtzen Claus Henrik Nielsen
author_sort	Chris Juul Hedegaard
title	Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
title_short	Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
title_full	Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
title_fullStr	Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
title_full_unstemmed	Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.
title_sort	variation in nod2 augments th2- and th17 responses to myelin basic protein in multiple sclerosis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2c36a59a22a64d47bd110bbaee8755ae
work_keys_str_mv	AT chrisjuulhedegaard variationinnod2augmentsth2andth17responsestomyelinbasicproteininmultiplesclerosis AT christianenevold variationinnod2augmentsth2andth17responsestomyelinbasicproteininmultiplesclerosis AT finnsellebjerg variationinnod2augmentsth2andth17responsestomyelinbasicproteininmultiplesclerosis AT klausbendtzen variationinnod2augmentsth2andth17responsestomyelinbasicproteininmultiplesclerosis AT claushenriknielsen variationinnod2augmentsth2andth17responsestomyelinbasicproteininmultiplesclerosis
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Variation in NOD2 augments Th2- and Th17 responses to myelin basic protein in multiple sclerosis.

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