Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).

In the present study, we have quantified the effects of transport, relocation and acclimate/adapt to their new surroundings on female squirrel monkey. These responses are measured in blood samples obtained from squirrel monkeys, at different time points relative to their relocation from their old ho...

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Autores principales:	Pramod N Nehete, Bharti P Nehete, Greg K Wilkerson, Steve J Schapiro, Lawrence E Williams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c3b35339e4f4ed49faac55a54eae491
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spelling	oai:doaj.org-article:2c3b35339e4f4ed49faac55a54eae4912021-12-02T20:11:26ZEffects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).1932-620310.1371/journal.pone.0240705https://doaj.org/article/2c3b35339e4f4ed49faac55a54eae4912021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0240705https://doaj.org/toc/1932-6203In the present study, we have quantified the effects of transport, relocation and acclimate/adapt to their new surroundings on female squirrel monkey. These responses are measured in blood samples obtained from squirrel monkeys, at different time points relative to their relocation from their old home to their new home. A group of squirrel monkeys we transported, by truck, for approximately 10 hours. Peripheral blood mononuclear cells (PBMCs) were assayed in order to evaluate the phenotype of lymphocyte subsets by flow, mitogen-specific immune responses of PBMCs in vitro, and levels of cytokines at various time points including immediately before transport, immediately upon arrival, and after approximately 150 days of acclimation. We observed significant changes in T cells and subsets, NK and B cells (CD4+, CD8+, CD4+/CD8+, CD16+, and CD20+). Mitogen specific (e.g. PHA, PWM and LPS) proliferation responses, IFN-γ by ELISPOT assay, and cytokines (IL-2, IL-4 and VEGF) significant changes were observed. Changes seen in the serum chemistry measurements mostly complement those seen in the hematology data. The specific goal was to empirically assess the effects of relocation stress in squirrel monkeys in terms of changes in the numbers and functions of various leukocyte subsets in the blood and the amount of time required for acclimating to their new environment. Such data will help to determine when newly arrived animals become available for use in research studies.Pramod N NeheteBharti P NeheteGreg K WilkersonSteve J SchapiroLawrence E WilliamsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 2, p e0240705 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pramod N Nehete Bharti P Nehete Greg K Wilkerson Steve J Schapiro Lawrence E Williams Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
description	In the present study, we have quantified the effects of transport, relocation and acclimate/adapt to their new surroundings on female squirrel monkey. These responses are measured in blood samples obtained from squirrel monkeys, at different time points relative to their relocation from their old home to their new home. A group of squirrel monkeys we transported, by truck, for approximately 10 hours. Peripheral blood mononuclear cells (PBMCs) were assayed in order to evaluate the phenotype of lymphocyte subsets by flow, mitogen-specific immune responses of PBMCs in vitro, and levels of cytokines at various time points including immediately before transport, immediately upon arrival, and after approximately 150 days of acclimation. We observed significant changes in T cells and subsets, NK and B cells (CD4+, CD8+, CD4+/CD8+, CD16+, and CD20+). Mitogen specific (e.g. PHA, PWM and LPS) proliferation responses, IFN-γ by ELISPOT assay, and cytokines (IL-2, IL-4 and VEGF) significant changes were observed. Changes seen in the serum chemistry measurements mostly complement those seen in the hematology data. The specific goal was to empirically assess the effects of relocation stress in squirrel monkeys in terms of changes in the numbers and functions of various leukocyte subsets in the blood and the amount of time required for acclimating to their new environment. Such data will help to determine when newly arrived animals become available for use in research studies.
format	article
author	Pramod N Nehete Bharti P Nehete Greg K Wilkerson Steve J Schapiro Lawrence E Williams
author_facet	Pramod N Nehete Bharti P Nehete Greg K Wilkerson Steve J Schapiro Lawrence E Williams
author_sort	Pramod N Nehete
title	Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
title_short	Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
title_full	Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
title_fullStr	Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
title_full_unstemmed	Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).
title_sort	effects of relocation on immunological and physiological measures in female squirrel monkeys (saimiri boliviensis boliviensis).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2c3b35339e4f4ed49faac55a54eae491
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Effects of relocation on immunological and physiological measures in female squirrel monkeys (Saimiri boliviensis boliviensis).

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