Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication

Abstract Aleutian mink disease (AMD), which is caused by Aleutian mink disease virus (AMDV), is an important contagious disease for which no effective vaccine is yet available. AMD causes major economic losses for mink farmers globally and threatens some carnivores such as skunks, genets, foxes and...

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Autores principales:	Taofeng Lu, Hui Zhang, Jie Zhou, Qin Ma, Wenzhuo Yan, Lili Zhao, Shuguang Wu, Hongyan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c3ed8bde37d47fcba4e250fc9313ea7
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spelling	oai:doaj.org-article:2c3ed8bde37d47fcba4e250fc9313ea72021-12-02T15:54:13ZAptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication10.1038/s41598-021-84223-82045-2322https://doaj.org/article/2c3ed8bde37d47fcba4e250fc9313ea72021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84223-8https://doaj.org/toc/2045-2322Abstract Aleutian mink disease (AMD), which is caused by Aleutian mink disease virus (AMDV), is an important contagious disease for which no effective vaccine is yet available. AMD causes major economic losses for mink farmers globally and threatens some carnivores such as skunks, genets, foxes and raccoons. Aptamers have exciting potential for the diagnosis and/or treatment of infectious viral diseases, including AMD. Using a magnetic beads-based systemic evolution of ligands by exponential enrichment (SELEX) approach, we have developed aptamers with activity against AMDV after 10 rounds of selection. After incubation with the ADVa012 aptamer (4 μM) for 48 h, the concentration of AMDV in the supernatant of infected cells was 47% lower than in the supernatant of untreated cells, whereas a random library of aptamers has no effect. The half-life of ADVa012 was ~ 32 h, which is significantly longer than that of other aptamers. Sequences and three dimensions structural modeling of selected aptamers indicated that they fold into similar stem-loop structures, which may be a preferred structure for binding to the target protein. The ADVa012 aptamer was shown to have an effective and long-lasting inhibitory effect on viral production in vitro.Taofeng LuHui ZhangJie ZhouQin MaWenzhuo YanLili ZhaoShuguang WuHongyan ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taofeng Lu Hui Zhang Jie Zhou Qin Ma Wenzhuo Yan Lili Zhao Shuguang Wu Hongyan Chen Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
description	Abstract Aleutian mink disease (AMD), which is caused by Aleutian mink disease virus (AMDV), is an important contagious disease for which no effective vaccine is yet available. AMD causes major economic losses for mink farmers globally and threatens some carnivores such as skunks, genets, foxes and raccoons. Aptamers have exciting potential for the diagnosis and/or treatment of infectious viral diseases, including AMD. Using a magnetic beads-based systemic evolution of ligands by exponential enrichment (SELEX) approach, we have developed aptamers with activity against AMDV after 10 rounds of selection. After incubation with the ADVa012 aptamer (4 μM) for 48 h, the concentration of AMDV in the supernatant of infected cells was 47% lower than in the supernatant of untreated cells, whereas a random library of aptamers has no effect. The half-life of ADVa012 was ~ 32 h, which is significantly longer than that of other aptamers. Sequences and three dimensions structural modeling of selected aptamers indicated that they fold into similar stem-loop structures, which may be a preferred structure for binding to the target protein. The ADVa012 aptamer was shown to have an effective and long-lasting inhibitory effect on viral production in vitro.
format	article
author	Taofeng Lu Hui Zhang Jie Zhou Qin Ma Wenzhuo Yan Lili Zhao Shuguang Wu Hongyan Chen
author_facet	Taofeng Lu Hui Zhang Jie Zhou Qin Ma Wenzhuo Yan Lili Zhao Shuguang Wu Hongyan Chen
author_sort	Taofeng Lu
title	Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
title_short	Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
title_full	Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
title_fullStr	Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
title_full_unstemmed	Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication
title_sort	aptamer-targeting of aleutian mink disease virus (amdv) can be an effective strategy to inhibit virus replication
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c3ed8bde37d47fcba4e250fc9313ea7
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Aptamer-targeting of Aleutian mink disease virus (AMDV) can be an effective strategy to inhibit virus replication

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