Some living eukaryotes during and after scanning electron microscopy

Abstract Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria...

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Auteur principal:	Ki Woo Kim
Format:	article
Langue:	EN
Publié:	SpringerOpen 2021
Sujets:	Desiccation Radiation Scanning electron microscopy Vacuum Microscopy QH201-278.5
Accès en ligne:	https://doaj.org/article/2c400e0563c3406aa6156f6eb95a90ea
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id	oai:doaj.org-article:2c400e0563c3406aa6156f6eb95a90ea
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spelling	oai:doaj.org-article:2c400e0563c3406aa6156f6eb95a90ea2021-11-08T11:04:33ZSome living eukaryotes during and after scanning electron microscopy10.1186/s42649-021-00065-82287-4445https://doaj.org/article/2c400e0563c3406aa6156f6eb95a90ea2021-11-01T00:00:00Zhttps://doi.org/10.1186/s42649-021-00065-8https://doaj.org/toc/2287-4445Abstract Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.Ki Woo KimSpringerOpenarticleDesiccationRadiationScanning electron microscopyVacuumMicroscopyQH201-278.5ENApplied Microscopy, Vol 51, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Desiccation Radiation Scanning electron microscopy Vacuum Microscopy QH201-278.5
spellingShingle	Desiccation Radiation Scanning electron microscopy Vacuum Microscopy QH201-278.5 Ki Woo Kim Some living eukaryotes during and after scanning electron microscopy
description	Abstract Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.
format	article
author	Ki Woo Kim
author_facet	Ki Woo Kim
author_sort	Ki Woo Kim
title	Some living eukaryotes during and after scanning electron microscopy
title_short	Some living eukaryotes during and after scanning electron microscopy
title_full	Some living eukaryotes during and after scanning electron microscopy
title_fullStr	Some living eukaryotes during and after scanning electron microscopy
title_full_unstemmed	Some living eukaryotes during and after scanning electron microscopy
title_sort	some living eukaryotes during and after scanning electron microscopy
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/2c400e0563c3406aa6156f6eb95a90ea
work_keys_str_mv	AT kiwookim somelivingeukaryotesduringandafterscanningelectronmicroscopy
_version_	1718442407823933440

Some living eukaryotes during and after scanning electron microscopy

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