Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths

Difficulty in controlling SARS-CoV-2 transmission made the ability to inactivate viruses in aerosols and fomites to be an important and attractive risk reduction measure. Evidence that light frequencies have the ability to inhibit microorganisms has already been reported by many studies which, howev...

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Autores principales:	Riccardo De Santis, Vincenzo Luca, Jonas Näslund, Rosina K. Ehmann, Marta De Angelis, Eva Lundmark, Lucia Nencioni, Giovanni Faggioni, Silvia Fillo, Donatella Amatore, Elisa Regalbuto, Filippo Molinari, Giancarlo Petralito, Roman Wölfel, Paola Stefanelli, Gianni Rezza, Anna Teresa Palamara, Markus Antwerpen, Mats Forsman, Florigio Lista
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	COVID-19 LED irradiation Inactivation SARS-CoV-2 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2c4253f0ce4f44eb9584776e1928c151
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spelling	oai:doaj.org-article:2c4253f0ce4f44eb9584776e1928c1512021-11-04T04:40:28ZRapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths2666-469010.1016/j.jpap.2021.100082https://doaj.org/article/2c4253f0ce4f44eb9584776e1928c1512021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666469021000671https://doaj.org/toc/2666-4690Difficulty in controlling SARS-CoV-2 transmission made the ability to inactivate viruses in aerosols and fomites to be an important and attractive risk reduction measure. Evidence that light frequencies have the ability to inhibit microorganisms has already been reported by many studies which, however, focused on ultraviolet (UV) wavelengths, which are known to induce potential injury in humans. In the present study, the effect on suspensions of SARS-CoV-2 of a Light Emitting Diode (LED) device capable of radiating frequencies in the non-hazardous visible light spectrum (VIS) was investigated. In order to evaluate the efficiency of viral inactivation, plaque assay and western blot of viral proteins were performed. The observed results showed a significant reduction in infectious particles that had been exposed to the LED irradiation of visible light. Furthermore, the analysis of the intracellular expression of viral proteins confirmed the inactivating effect of this irradiation technology. This in vitro study revealed for the first time the inactivation of SARS-CoV-2 through LED irradiation with multiple wavelengths of the visible spectrum. However additional and more in-depth studies can aim to demonstrate the data obtained during these experiments in different matrices, in mutable environmental conditions and on other respiratory viruses such as the influenza virus. The type of LED technology can decisively contribute on reducing virus transmission through the continuous sanitation of common environments without risks for humans and animals.Riccardo De SantisVincenzo LucaJonas NäslundRosina K. EhmannMarta De AngelisEva LundmarkLucia NencioniGiovanni FaggioniSilvia FilloDonatella AmatoreElisa RegalbutoFilippo MolinariGiancarlo PetralitoRoman WölfelPaola StefanelliGianni RezzaAnna Teresa PalamaraMarkus AntwerpenMats ForsmanFlorigio ListaElsevierarticleCOVID-19LED irradiationInactivationSARS-CoV-2ChemistryQD1-999ENJournal of Photochemistry and Photobiology, Vol 8, Iss , Pp 100082- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 LED irradiation Inactivation SARS-CoV-2 Chemistry QD1-999
spellingShingle	COVID-19 LED irradiation Inactivation SARS-CoV-2 Chemistry QD1-999 Riccardo De Santis Vincenzo Luca Jonas Näslund Rosina K. Ehmann Marta De Angelis Eva Lundmark Lucia Nencioni Giovanni Faggioni Silvia Fillo Donatella Amatore Elisa Regalbuto Filippo Molinari Giancarlo Petralito Roman Wölfel Paola Stefanelli Gianni Rezza Anna Teresa Palamara Markus Antwerpen Mats Forsman Florigio Lista Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
description	Difficulty in controlling SARS-CoV-2 transmission made the ability to inactivate viruses in aerosols and fomites to be an important and attractive risk reduction measure. Evidence that light frequencies have the ability to inhibit microorganisms has already been reported by many studies which, however, focused on ultraviolet (UV) wavelengths, which are known to induce potential injury in humans. In the present study, the effect on suspensions of SARS-CoV-2 of a Light Emitting Diode (LED) device capable of radiating frequencies in the non-hazardous visible light spectrum (VIS) was investigated. In order to evaluate the efficiency of viral inactivation, plaque assay and western blot of viral proteins were performed. The observed results showed a significant reduction in infectious particles that had been exposed to the LED irradiation of visible light. Furthermore, the analysis of the intracellular expression of viral proteins confirmed the inactivating effect of this irradiation technology. This in vitro study revealed for the first time the inactivation of SARS-CoV-2 through LED irradiation with multiple wavelengths of the visible spectrum. However additional and more in-depth studies can aim to demonstrate the data obtained during these experiments in different matrices, in mutable environmental conditions and on other respiratory viruses such as the influenza virus. The type of LED technology can decisively contribute on reducing virus transmission through the continuous sanitation of common environments without risks for humans and animals.
format	article
author	Riccardo De Santis Vincenzo Luca Jonas Näslund Rosina K. Ehmann Marta De Angelis Eva Lundmark Lucia Nencioni Giovanni Faggioni Silvia Fillo Donatella Amatore Elisa Regalbuto Filippo Molinari Giancarlo Petralito Roman Wölfel Paola Stefanelli Gianni Rezza Anna Teresa Palamara Markus Antwerpen Mats Forsman Florigio Lista
author_facet	Riccardo De Santis Vincenzo Luca Jonas Näslund Rosina K. Ehmann Marta De Angelis Eva Lundmark Lucia Nencioni Giovanni Faggioni Silvia Fillo Donatella Amatore Elisa Regalbuto Filippo Molinari Giancarlo Petralito Roman Wölfel Paola Stefanelli Gianni Rezza Anna Teresa Palamara Markus Antwerpen Mats Forsman Florigio Lista
author_sort	Riccardo De Santis
title	Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
title_short	Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
title_full	Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
title_fullStr	Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
title_full_unstemmed	Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths
title_sort	rapid inactivation of sars-cov-2 with led irradiation of visible spectrum wavelengths
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2c4253f0ce4f44eb9584776e1928c151
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Rapid inactivation of SARS-CoV-2 with LED irradiation of visible spectrum wavelengths

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