Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil

Abstract A deep-sea fungus Aspergillus sydowii BOBA1 isolated from marine sediment at a depth of 3000 m was capable of degrading spent engine (SE) oil. The response of immobilized fungi towards degradation at elevated pressure was studied in customized high pressure reactors without any deviation in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. Ganesh Kumar, D. Manisha, K. Sujitha, D. Magesh Peter, R. Kirubagaran, G. Dharani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c497db0350c45d391f66f9d27555b4c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c497db0350c45d391f66f9d27555b4c
record_format	dspace
spelling	oai:doaj.org-article:2c497db0350c45d391f66f9d27555b4c2021-12-02T13:41:10ZGenome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil10.1038/s41598-021-88525-92045-2322https://doaj.org/article/2c497db0350c45d391f66f9d27555b4c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88525-9https://doaj.org/toc/2045-2322Abstract A deep-sea fungus Aspergillus sydowii BOBA1 isolated from marine sediment at a depth of 3000 m was capable of degrading spent engine (SE) oil. The response of immobilized fungi towards degradation at elevated pressure was studied in customized high pressure reactors without any deviation in simulating in situ deep-sea conditions. The growth rate of A. sydowii BOBA1 in 0.1 MPa was significantly different from the growth at 10 MPa pressure. The degradation percentage reached 71.2 and 82.5% at atmospheric and high pressure conditions, respectively, within a retention period of 21 days. The complete genome sequence of BOBA1 consists of 38,795,664 bp in size, comprises 2582 scaffolds with predicted total coding genes of 18,932. A total of 16,247 genes were assigned with known functions and many families found to have a potential role in PAHs and xenobiotic compound metabolism. Functional genes controlling the pathways of hydrocarbon and xenobiotics compound degrading enzymes such as dioxygenase, decarboxylase, hydrolase, reductase and peroxidase were identified. The spectroscopic and genomic analysis revealed the presence of combined catechol, gentisate and phthalic acid degradation pathway. These results of degradation and genomic studies evidenced that this deep-sea fungus could be employed to develop an eco-friendly mycoremediation technology to combat the oil polluted marine environment. This study expands our knowledge on piezophilic fungi and offer insight into possibilities about the fate of SE oil in deep-sea.A. Ganesh KumarD. ManishaK. SujithaD. Magesh PeterR. KirubagaranG. DharaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Ganesh Kumar D. Manisha K. Sujitha D. Magesh Peter R. Kirubagaran G. Dharani Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
description	Abstract A deep-sea fungus Aspergillus sydowii BOBA1 isolated from marine sediment at a depth of 3000 m was capable of degrading spent engine (SE) oil. The response of immobilized fungi towards degradation at elevated pressure was studied in customized high pressure reactors without any deviation in simulating in situ deep-sea conditions. The growth rate of A. sydowii BOBA1 in 0.1 MPa was significantly different from the growth at 10 MPa pressure. The degradation percentage reached 71.2 and 82.5% at atmospheric and high pressure conditions, respectively, within a retention period of 21 days. The complete genome sequence of BOBA1 consists of 38,795,664 bp in size, comprises 2582 scaffolds with predicted total coding genes of 18,932. A total of 16,247 genes were assigned with known functions and many families found to have a potential role in PAHs and xenobiotic compound metabolism. Functional genes controlling the pathways of hydrocarbon and xenobiotics compound degrading enzymes such as dioxygenase, decarboxylase, hydrolase, reductase and peroxidase were identified. The spectroscopic and genomic analysis revealed the presence of combined catechol, gentisate and phthalic acid degradation pathway. These results of degradation and genomic studies evidenced that this deep-sea fungus could be employed to develop an eco-friendly mycoremediation technology to combat the oil polluted marine environment. This study expands our knowledge on piezophilic fungi and offer insight into possibilities about the fate of SE oil in deep-sea.
format	article
author	A. Ganesh Kumar D. Manisha K. Sujitha D. Magesh Peter R. Kirubagaran G. Dharani
author_facet	A. Ganesh Kumar D. Manisha K. Sujitha D. Magesh Peter R. Kirubagaran G. Dharani
author_sort	A. Ganesh Kumar
title	Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
title_short	Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
title_full	Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
title_fullStr	Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
title_full_unstemmed	Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil
title_sort	genome sequence analysis of deep sea aspergillus sydowii boba1 and effect of high pressure on biodegradation of spent engine oil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c497db0350c45d391f66f9d27555b4c
work_keys_str_mv	AT aganeshkumar genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil AT dmanisha genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil AT ksujitha genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil AT dmageshpeter genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil AT rkirubagaran genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil AT gdharani genomesequenceanalysisofdeepseaaspergillussydowiiboba1andeffectofhighpressureonbiodegradationofspentengineoil
_version_	1718392606620123136

Genome sequence analysis of deep sea Aspergillus sydowii BOBA1 and effect of high pressure on biodegradation of spent engine oil

Ejemplares similares