Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode

Priyono DS, Solihin DD, Farajallah A, Arini DID. 2018. Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode. Biodiversitas 19: 1985-1992. Anoa is an endangered endemic species in Sulawesi. The controversial issue of anoa conservation until now is the taxonomic status of...

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Autores principales:	DWI SENDI PRIYONO, DEDY D. SOLIHIN, ACHMAD FARAJALLAH, DIAH IRAWATI DWI ARINI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	anoa coi dna barcoding phylogenetic molecular taxonomy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2c4ae47ffbc94cb99294fa88c1cb865a
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spelling	oai:doaj.org-article:2c4ae47ffbc94cb99294fa88c1cb865a2021-11-16T14:04:41ZAnoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode1412-033X2085-472210.13057/biodiv/d190602https://doaj.org/article/2c4ae47ffbc94cb99294fa88c1cb865a2018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3010https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Priyono DS, Solihin DD, Farajallah A, Arini DID. 2018. Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode. Biodiversitas 19: 1985-1992. Anoa is an endangered endemic species in Sulawesi. The controversial issue of anoa conservation until now is the taxonomic status of lowland and mountain anoa. This study aims to test the ability of DNA barcoding techniques to identify the taxonomy between mountain anoa and lowland anoa. A 681bp fragment of cytochrome oxidase subunit 1 (COI) gene was obtained and used to solve the molecular taxonomic problem and to resolve the phylogenetic relationships of the two types of anoa. Our results showed that the DNA barcode is useful in assigning the taxonomic position of anoa. In the phylogenetic tree, we found that the two types of anoa were in separate clades. We also found that based on the Kimura-2 parameter (K2P), the genetic distance between the two types of anoa showed higher values (3.4%) than the threshold of the separating species level. We, therefore, proposed that the binomial nomenclature for both types of lowland and mountain anoa are respectively Bubalus depressicornis and Bubalus quarlesi. We suggest that the use of DNA barcode techniques in anoa taxonomic studies and their implementation will be useful in conservation managementDWI SENDI PRIYONODEDY D. SOLIHINACHMAD FARAJALLAHDIAH IRAWATI DWI ARINIMBI & UNS Soloarticleanoacoidna barcodingphylogeneticmolecular taxonomyBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 1985-1992 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	anoa coi dna barcoding phylogenetic molecular taxonomy Biology (General) QH301-705.5
spellingShingle	anoa coi dna barcoding phylogenetic molecular taxonomy Biology (General) QH301-705.5 DWI SENDI PRIYONO DEDY D. SOLIHIN ACHMAD FARAJALLAH DIAH IRAWATI DWI ARINI Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
description	Priyono DS, Solihin DD, Farajallah A, Arini DID. 2018. Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode. Biodiversitas 19: 1985-1992. Anoa is an endangered endemic species in Sulawesi. The controversial issue of anoa conservation until now is the taxonomic status of lowland and mountain anoa. This study aims to test the ability of DNA barcoding techniques to identify the taxonomy between mountain anoa and lowland anoa. A 681bp fragment of cytochrome oxidase subunit 1 (COI) gene was obtained and used to solve the molecular taxonomic problem and to resolve the phylogenetic relationships of the two types of anoa. Our results showed that the DNA barcode is useful in assigning the taxonomic position of anoa. In the phylogenetic tree, we found that the two types of anoa were in separate clades. We also found that based on the Kimura-2 parameter (K2P), the genetic distance between the two types of anoa showed higher values (3.4%) than the threshold of the separating species level. We, therefore, proposed that the binomial nomenclature for both types of lowland and mountain anoa are respectively Bubalus depressicornis and Bubalus quarlesi. We suggest that the use of DNA barcode techniques in anoa taxonomic studies and their implementation will be useful in conservation management
format	article
author	DWI SENDI PRIYONO DEDY D. SOLIHIN ACHMAD FARAJALLAH DIAH IRAWATI DWI ARINI
author_facet	DWI SENDI PRIYONO DEDY D. SOLIHIN ACHMAD FARAJALLAH DIAH IRAWATI DWI ARINI
author_sort	DWI SENDI PRIYONO
title	Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
title_short	Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
title_full	Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
title_fullStr	Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
title_full_unstemmed	Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode
title_sort	anoa, dwarf buffalo from sulawesi, indonesia: identification based on dna barcode
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/2c4ae47ffbc94cb99294fa88c1cb865a
work_keys_str_mv	AT dwisendipriyono anoadwarfbuffalofromsulawesiindonesiaidentificationbasedondnabarcode AT dedydsolihin anoadwarfbuffalofromsulawesiindonesiaidentificationbasedondnabarcode AT achmadfarajallah anoadwarfbuffalofromsulawesiindonesiaidentificationbasedondnabarcode AT diahirawatidwiarini anoadwarfbuffalofromsulawesiindonesiaidentificationbasedondnabarcode
_version_	1718426347669291008

Anoa, dwarf buffalo from Sulawesi, Indonesia: Identification based on DNA barcode

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