Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.

Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vergiana Dos Santos Paixão, Pablo Suárez, Willam Oliveira da Silva, Lena Geise, Malcolm Andrew Ferguson-Smith, Patricia Caroline Mary O'Brien, Ana Cristina Mendes-Oliveira, Rogério Vieira Rossi, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c565001f22244628b4003c18993e976
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c565001f22244628b4003c18993e976
record_format	dspace
spelling	oai:doaj.org-article:2c565001f22244628b4003c18993e9762021-12-02T20:17:03ZComparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.1932-620310.1371/journal.pone.0258474https://doaj.org/article/2c565001f22244628b4003c18993e9762021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258474https://doaj.org/toc/1932-6203Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associated with karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.Vergiana Dos Santos PaixãoPablo SuárezWillam Oliveira da SilvaLena GeiseMalcolm Andrew Ferguson-SmithPatricia Caroline Mary O'BrienAna Cristina Mendes-OliveiraRogério Vieira RossiJulio Cesar PieczarkaCleusa Yoshiko NagamachiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258474 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vergiana Dos Santos Paixão Pablo Suárez Willam Oliveira da Silva Lena Geise Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Ana Cristina Mendes-Oliveira Rogério Vieira Rossi Julio Cesar Pieczarka Cleusa Yoshiko Nagamachi Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
description	Rhipidomys (Sigmodontinae, Thomasomyini) has 25 recognized species, with a wide distribution ranging from eastern Panama to northern Argentina. Cytogenetic data has been described for 13 species with 12 of them having 2n = 44 with a high level of autosomal fundamental number (FN) variation, ranging from 46 to 80, assigned to pericentric inversions. The species are grouped in groups with low FN (46-52) and high FN (72-80). In this work the karyotypes of Rhipidomys emiliae (2n = 44, FN = 50) and Rhipidomys mastacalis (2n = 44, FN = 74), were studied by classical cytogenetics and by fluorescence in situ hybridization using telomeric and whole chromosome probes (chromosome painting) of Hylaeamys megacephalus (HME). Chromosome painting revealed homology between 36 segments of REM and 37 of RMA. We tested the hypothesis that pericentric inversions are the predominant chromosomal rearrangements responsible for karyotypic divergence between these species, as proposed in literature. Our results show that the genomic diversification between the karyotypes of the two species resulted from translocations, centromeric repositioning and pericentric inversions. The chromosomal evolution in Rhipidomys was associated with karyotypical orthoselection. The HME probes revealed that seven syntenic probably ancestral blocks for Sigmodontinae are present in Rhipidomys. An additional syntenic block described here is suggested as part of the subfamily ancestral karyotype. We also define five synapomorphies that can be used as chromosomal signatures for Rhipidomys.
format	article
author	Vergiana Dos Santos Paixão Pablo Suárez Willam Oliveira da Silva Lena Geise Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Ana Cristina Mendes-Oliveira Rogério Vieira Rossi Julio Cesar Pieczarka Cleusa Yoshiko Nagamachi
author_facet	Vergiana Dos Santos Paixão Pablo Suárez Willam Oliveira da Silva Lena Geise Malcolm Andrew Ferguson-Smith Patricia Caroline Mary O'Brien Ana Cristina Mendes-Oliveira Rogério Vieira Rossi Julio Cesar Pieczarka Cleusa Yoshiko Nagamachi
author_sort	Vergiana Dos Santos Paixão
title	Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
title_short	Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
title_full	Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
title_fullStr	Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
title_full_unstemmed	Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.
title_sort	comparative genomic mapping reveals mechanisms of chromosome diversification in rhipidomys species (rodentia, thomasomyini) and syntenic relationship between species of sigmodontinae.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2c565001f22244628b4003c18993e976
work_keys_str_mv	AT vergianadossantospaixao comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT pablosuarez comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT willamoliveiradasilva comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT lenageise comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT malcolmandrewfergusonsmith comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT patriciacarolinemaryobrien comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT anacristinamendesoliveira comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT rogeriovieirarossi comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT juliocesarpieczarka comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae AT cleusayoshikonagamachi comparativegenomicmappingrevealsmechanismsofchromosomediversificationinrhipidomysspeciesrodentiathomasomyiniandsyntenicrelationshipbetweenspeciesofsigmodontinae
_version_	1718374395556134912

Comparative genomic mapping reveals mechanisms of chromosome diversification in Rhipidomys species (Rodentia, Thomasomyini) and syntenic relationship between species of Sigmodontinae.

Ejemplares similares