INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE

B regulatory cells (Bregs) are shown to downregulate autoimmune and inflammation processes. Their modifying effects depend on IL-10 secretion. A role of Bregs in development of humoral immune response was not investigated. Influence of Bregs and IL-10 upon in vitro response of murine B1 and B2 cells...

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Autores principales:	M. V. Gavrilova, N. A. Snegireva, E. V. Sidorova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	bregs il-10 in vitro immune response induction antibody producers antigens b1-lymphocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2c5f86ff956e4f11b0be58b7e828aabb
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spelling	oai:doaj.org-article:2c5f86ff956e4f11b0be58b7e828aabb2021-11-18T08:03:45ZINFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE1563-06252313-741X10.15789/1563-0625-2016-4-331-338https://doaj.org/article/2c5f86ff956e4f11b0be58b7e828aabb2016-08-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1055https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XB regulatory cells (Bregs) are shown to downregulate autoimmune and inflammation processes. Their modifying effects depend on IL-10 secretion. A role of Bregs in development of humoral immune response was not investigated. Influence of Bregs and IL-10 upon in vitro response of murine B1 and B2 cells to T-dependent and T-independent antigens was studied in a model system. A water-soluble sheep erythrocyte antigen was used as a T-dependent antigen, whereas LPS was applied as a type 1 T-independent antigen, and polyvinylpirrolidone and alpha(1→3)dextran were added as type 2 T-independent antigens. В1and B2 lymphocytes were isolated from, respectively, peritoneal cavity and spleen of CBA mice. The cells were cultured in RPMI1640 medium with 10% of FCS supplemented with appropriate antigens and IL-10. The numbers of antibody- and total Ig-forming cells were determined by ELISPOT method.The erythrocyte antigen induced an increase of antibody- and total Ig-forming cell numbers in cultured B1 and B2 cell populations. IL-10 addition caused reduction of antibody- and total Ig-forming cells by 27%. Similarly, IL-10 caused a drop in antibody- and total Ig-forming cells in LPS-stimulated B2 cell cultures by 75%, as well as 50 per cent decrease in numbers of antibody-forming cells in B-1 cell cultures when induced by the type 2 T-independent antigens.To assess functional activity of Bregs, the cells were isolated from peritoneal cavity and spleen of CBA mice. Total yields of Bregs were 20-fold increased upon activation of B cells with LPS, ionomycin and phorbol ester (from 4% to 96%). IgM was the main immunoglobulin isotype secreted by the Bregs. 96% of activated Bregs produced IL-10. About 12% of the cells were shown to produce immunoglobulins. This finding suggests that some of Bregs synthesize both IL-10 and immunoglobulins.To study distant effect of Bregs upon immune response, the splenocyte culture of xid CBA/N mice were tested in Transwells with enriched Bregs. It was revealed that the Bregs caused inhibition of both specific and polyclonal immune response to the water-soluble sheep erythrocyte antigen. Hence, Bregs are shown to participate in humoral immune response, probably, by suppressing functional activity of splenocytes from CBA/N mice to T-dependent antigen. IL-10 secreted by Bregs may play a sufficient role in these regulatory effects.M. V. GavrilovaN. A. SnegirevaE. V. SidorovaSPb RAACIarticlebregsil-10in vitro immune response inductionantibody producersantigensb1-lymphocytesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 4, Pp 331-338 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	bregs il-10 in vitro immune response induction antibody producers antigens b1-lymphocytes Immunologic diseases. Allergy RC581-607
spellingShingle	bregs il-10 in vitro immune response induction antibody producers antigens b1-lymphocytes Immunologic diseases. Allergy RC581-607 M. V. Gavrilova N. A. Snegireva E. V. Sidorova INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
description	B regulatory cells (Bregs) are shown to downregulate autoimmune and inflammation processes. Their modifying effects depend on IL-10 secretion. A role of Bregs in development of humoral immune response was not investigated. Influence of Bregs and IL-10 upon in vitro response of murine B1 and B2 cells to T-dependent and T-independent antigens was studied in a model system. A water-soluble sheep erythrocyte antigen was used as a T-dependent antigen, whereas LPS was applied as a type 1 T-independent antigen, and polyvinylpirrolidone and alpha(1→3)dextran were added as type 2 T-independent antigens. В1and B2 lymphocytes were isolated from, respectively, peritoneal cavity and spleen of CBA mice. The cells were cultured in RPMI1640 medium with 10% of FCS supplemented with appropriate antigens and IL-10. The numbers of antibody- and total Ig-forming cells were determined by ELISPOT method.The erythrocyte antigen induced an increase of antibody- and total Ig-forming cell numbers in cultured B1 and B2 cell populations. IL-10 addition caused reduction of antibody- and total Ig-forming cells by 27%. Similarly, IL-10 caused a drop in antibody- and total Ig-forming cells in LPS-stimulated B2 cell cultures by 75%, as well as 50 per cent decrease in numbers of antibody-forming cells in B-1 cell cultures when induced by the type 2 T-independent antigens.To assess functional activity of Bregs, the cells were isolated from peritoneal cavity and spleen of CBA mice. Total yields of Bregs were 20-fold increased upon activation of B cells with LPS, ionomycin and phorbol ester (from 4% to 96%). IgM was the main immunoglobulin isotype secreted by the Bregs. 96% of activated Bregs produced IL-10. About 12% of the cells were shown to produce immunoglobulins. This finding suggests that some of Bregs synthesize both IL-10 and immunoglobulins.To study distant effect of Bregs upon immune response, the splenocyte culture of xid CBA/N mice were tested in Transwells with enriched Bregs. It was revealed that the Bregs caused inhibition of both specific and polyclonal immune response to the water-soluble sheep erythrocyte antigen. Hence, Bregs are shown to participate in humoral immune response, probably, by suppressing functional activity of splenocytes from CBA/N mice to T-dependent antigen. IL-10 secreted by Bregs may play a sufficient role in these regulatory effects.
format	article
author	M. V. Gavrilova N. A. Snegireva E. V. Sidorova
author_facet	M. V. Gavrilova N. A. Snegireva E. V. Sidorova
author_sort	M. V. Gavrilova
title	INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
title_short	INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
title_full	INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
title_fullStr	INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
title_full_unstemmed	INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE
title_sort	influence of breg and il-10 upon humoral immune response
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/2c5f86ff956e4f11b0be58b7e828aabb
work_keys_str_mv	AT mvgavrilova influenceofbregandil10uponhumoralimmuneresponse AT nasnegireva influenceofbregandil10uponhumoralimmuneresponse AT evsidorova influenceofbregandil10uponhumoralimmuneresponse
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INFLUENCE OF Breg AND IL-10 UPON HUMORAL IMMUNE RESPONSE

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