Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

The cyanophage S-2L incorporates 2-aminoadenine (Z) instead of adenine into its DNA, which still pairs with thymine forming a triple hydrogen bond. Here, the authors identify a third gene mazZ located between purZ and datZ that is required for 2-aminoadenine biosynthesis and determine the crystal st...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dariusz Czernecki, Frédéric Bonhomme, Pierre-Alexandre Kaminski, Marc Delarue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2c5f8be15bf64c02b05de1d016baf972
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c5f8be15bf64c02b05de1d016baf972
record_format	dspace
spelling	oai:doaj.org-article:2c5f8be15bf64c02b05de1d016baf9722021-12-02T16:35:42ZCharacterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA10.1038/s41467-021-25064-x2041-1723https://doaj.org/article/2c5f8be15bf64c02b05de1d016baf9722021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25064-xhttps://doaj.org/toc/2041-1723The cyanophage S-2L incorporates 2-aminoadenine (Z) instead of adenine into its DNA, which still pairs with thymine forming a triple hydrogen bond. Here, the authors identify a third gene mazZ located between purZ and datZ that is required for 2-aminoadenine biosynthesis and determine the crystal structures of MazZ and PurZ. They further show that co-expression of these three genes in E.coli enables 2-aminoadenine incorporation into the bacterial genome.Dariusz CzerneckiFrédéric BonhommePierre-Alexandre KaminskiMarc DelarueNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dariusz Czernecki Frédéric Bonhomme Pierre-Alexandre Kaminski Marc Delarue Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
description	The cyanophage S-2L incorporates 2-aminoadenine (Z) instead of adenine into its DNA, which still pairs with thymine forming a triple hydrogen bond. Here, the authors identify a third gene mazZ located between purZ and datZ that is required for 2-aminoadenine biosynthesis and determine the crystal structures of MazZ and PurZ. They further show that co-expression of these three genes in E.coli enables 2-aminoadenine incorporation into the bacterial genome.
format	article
author	Dariusz Czernecki Frédéric Bonhomme Pierre-Alexandre Kaminski Marc Delarue
author_facet	Dariusz Czernecki Frédéric Bonhomme Pierre-Alexandre Kaminski Marc Delarue
author_sort	Dariusz Czernecki
title	Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
title_short	Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
title_full	Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
title_fullStr	Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
title_full_unstemmed	Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA
title_sort	characterization of a triad of genes in cyanophage s-2l sufficient to replace adenine by 2-aminoadenine in bacterial dna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c5f8be15bf64c02b05de1d016baf972
work_keys_str_mv	AT dariuszczernecki characterizationofatriadofgenesincyanophages2lsufficienttoreplaceadenineby2aminoadenineinbacterialdna AT fredericbonhomme characterizationofatriadofgenesincyanophages2lsufficienttoreplaceadenineby2aminoadenineinbacterialdna AT pierrealexandrekaminski characterizationofatriadofgenesincyanophages2lsufficienttoreplaceadenineby2aminoadenineinbacterialdna AT marcdelarue characterizationofatriadofgenesincyanophages2lsufficienttoreplaceadenineby2aminoadenineinbacterialdna
_version_	1718383682209710080

Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

Ejemplares similares