In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium

Abstract Melanosomes, lipofuscin, and melanolipofuscin are the three principal types of pigmented granules found in retinal pigment epithelium (RPE) cells. Changes in the density of melanosomes and lipofuscin in RPE cells are considered hallmarks of various retinal diseases, including Stargardt dise...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ratheesh K. Meleppat, Kaitryn E. Ronning, Sarah J. Karlen, Marie E. Burns, Edward N. Pugh, Robert J. Zawadzki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c70fd6f2c07406299a4c1fd0298f7e8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c70fd6f2c07406299a4c1fd0298f7e8
record_format	dspace
spelling	oai:doaj.org-article:2c70fd6f2c07406299a4c1fd0298f7e82021-12-02T18:50:49ZIn vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium10.1038/s41598-021-95320-z2045-2322https://doaj.org/article/2c70fd6f2c07406299a4c1fd0298f7e82021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95320-zhttps://doaj.org/toc/2045-2322Abstract Melanosomes, lipofuscin, and melanolipofuscin are the three principal types of pigmented granules found in retinal pigment epithelium (RPE) cells. Changes in the density of melanosomes and lipofuscin in RPE cells are considered hallmarks of various retinal diseases, including Stargardt disease and age-related macular degeneration (AMD). Herein, we report the potential of an in vivo multimodal imaging technique based on directional back-scattering and short-wavelength fundus autofluorescence (SW-FAF) to study disease-related changes in the density of melanosomes and lipofuscin granules in RPE cells. Changes in the concentration of these granules in Abca4 −/− mice (a model of Stargardt disease) relative to age-matched wild-type (WT) controls were investigated. Directional optical coherence tomography (dOCT) was used to assess melanosome density in vivo, whereas the autofluorescence (AF) images and emission spectra acquired with a spectrometer-integrated scanning laser ophthalmoscope (SLO) were used to characterize lipofuscin and melanolipofuscin granules in the same RPE region. Subcellular-resolution ex vivo imaging using confocal fluorescence microscopy and electron microscopy was performed on the same tissue region to visualize and quantify melanosomes, lipofuscin, and melanolipofuscin granules. Comparisons between in vivo and ex vivo results confirmed an increased concentration of lipofuscin granules and decreased concentration of melanosomes in the RPE of Abca4 −/− mice, and provided an explanation for the differences in fluorescence and directionality of RPE scattering observed in vivo between the two mouse strains.Ratheesh K. MeleppatKaitryn E. RonningSarah J. KarlenMarie E. BurnsEdward N. PughRobert J. ZawadzkiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ratheesh K. Meleppat Kaitryn E. Ronning Sarah J. Karlen Marie E. Burns Edward N. Pugh Robert J. Zawadzki In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
description	Abstract Melanosomes, lipofuscin, and melanolipofuscin are the three principal types of pigmented granules found in retinal pigment epithelium (RPE) cells. Changes in the density of melanosomes and lipofuscin in RPE cells are considered hallmarks of various retinal diseases, including Stargardt disease and age-related macular degeneration (AMD). Herein, we report the potential of an in vivo multimodal imaging technique based on directional back-scattering and short-wavelength fundus autofluorescence (SW-FAF) to study disease-related changes in the density of melanosomes and lipofuscin granules in RPE cells. Changes in the concentration of these granules in Abca4 −/− mice (a model of Stargardt disease) relative to age-matched wild-type (WT) controls were investigated. Directional optical coherence tomography (dOCT) was used to assess melanosome density in vivo, whereas the autofluorescence (AF) images and emission spectra acquired with a spectrometer-integrated scanning laser ophthalmoscope (SLO) were used to characterize lipofuscin and melanolipofuscin granules in the same RPE region. Subcellular-resolution ex vivo imaging using confocal fluorescence microscopy and electron microscopy was performed on the same tissue region to visualize and quantify melanosomes, lipofuscin, and melanolipofuscin granules. Comparisons between in vivo and ex vivo results confirmed an increased concentration of lipofuscin granules and decreased concentration of melanosomes in the RPE of Abca4 −/− mice, and provided an explanation for the differences in fluorescence and directionality of RPE scattering observed in vivo between the two mouse strains.
format	article
author	Ratheesh K. Meleppat Kaitryn E. Ronning Sarah J. Karlen Marie E. Burns Edward N. Pugh Robert J. Zawadzki
author_facet	Ratheesh K. Meleppat Kaitryn E. Ronning Sarah J. Karlen Marie E. Burns Edward N. Pugh Robert J. Zawadzki
author_sort	Ratheesh K. Meleppat
title	In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
title_short	In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
title_full	In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
title_fullStr	In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
title_full_unstemmed	In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
title_sort	in vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c70fd6f2c07406299a4c1fd0298f7e8
work_keys_str_mv	AT ratheeshkmeleppat invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium AT kaitryneronning invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium AT sarahjkarlen invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium AT marieeburns invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium AT edwardnpugh invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium AT robertjzawadzki invivomultimodalretinalimagingofdiseaserelatedpigmentarychangesinretinalpigmentepithelium
_version_	1718377494092972032

In vivo multimodal retinal imaging of disease-related pigmentary changes in retinal pigment epithelium

Ejemplares similares