Quantitative phase microscopy for non-invasive live cell population monitoring

Abstract We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microsc...

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Autores principales:	Sherazade Aknoun, Manuel Yonnet, Zied Djabari, Fanny Graslin, Mark Taylor, Thierry Pourcher, Benoit Wattellier, Philippe Pognonec
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c79143620324a668954d4a7646be3b4
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spelling	oai:doaj.org-article:2c79143620324a668954d4a7646be3b42021-12-02T13:19:21ZQuantitative phase microscopy for non-invasive live cell population monitoring10.1038/s41598-021-83537-x2045-2322https://doaj.org/article/2c79143620324a668954d4a7646be3b42021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83537-xhttps://doaj.org/toc/2045-2322Abstract We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microscope. We show that considering the global biomass of a cell population is a more robust and accurate method to assess its growth parameters in comparison to compiling individually segmented cells. This is especially true for confluent conditions. This method expands the use of light microscopy in answering biological questions concerning live cell populations even at high density. In contrast to labeling or lysis of cells this method does not alter the cells and could be useful in high-throughput screening and toxicity studies.Sherazade AknounManuel YonnetZied DjabariFanny GraslinMark TaylorThierry PourcherBenoit WattellierPhilippe PognonecNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sherazade Aknoun Manuel Yonnet Zied Djabari Fanny Graslin Mark Taylor Thierry Pourcher Benoit Wattellier Philippe Pognonec Quantitative phase microscopy for non-invasive live cell population monitoring
description	Abstract We present here a label-free development based on preexisting Quantitative Phase Imaging (QPI) that allows non-invasive live monitoring of both individual cells and cell populations. Growth, death, effect of toxic compounds are quantified under visible light with a standard inverted microscope. We show that considering the global biomass of a cell population is a more robust and accurate method to assess its growth parameters in comparison to compiling individually segmented cells. This is especially true for confluent conditions. This method expands the use of light microscopy in answering biological questions concerning live cell populations even at high density. In contrast to labeling or lysis of cells this method does not alter the cells and could be useful in high-throughput screening and toxicity studies.
format	article
author	Sherazade Aknoun Manuel Yonnet Zied Djabari Fanny Graslin Mark Taylor Thierry Pourcher Benoit Wattellier Philippe Pognonec
author_facet	Sherazade Aknoun Manuel Yonnet Zied Djabari Fanny Graslin Mark Taylor Thierry Pourcher Benoit Wattellier Philippe Pognonec
author_sort	Sherazade Aknoun
title	Quantitative phase microscopy for non-invasive live cell population monitoring
title_short	Quantitative phase microscopy for non-invasive live cell population monitoring
title_full	Quantitative phase microscopy for non-invasive live cell population monitoring
title_fullStr	Quantitative phase microscopy for non-invasive live cell population monitoring
title_full_unstemmed	Quantitative phase microscopy for non-invasive live cell population monitoring
title_sort	quantitative phase microscopy for non-invasive live cell population monitoring
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c79143620324a668954d4a7646be3b4
work_keys_str_mv	AT sherazadeaknoun quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT manuelyonnet quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT zieddjabari quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT fannygraslin quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT marktaylor quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT thierrypourcher quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT benoitwattellier quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring AT philippepognonec quantitativephasemicroscopyfornoninvasivelivecellpopulationmonitoring
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Quantitative phase microscopy for non-invasive live cell population monitoring

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