Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency

Sheng Wang,1,* Xiu M Mo,2,* Bo J Jiang,1 Cheng J Gao,1 Hong S Wang,2 Yu G Zhuang,1 Li J Qiu21Department of Emergency and Critical Care Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, People's Republic of China; 2State Key Laboratory for Modification of Chem...

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Autores principales:	Wang S, Mo XM, Jiang BJ, Gao CJ, Wang HS, Zhuang YG, Qiu LJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2c7e2d18f9b541139797761f5a888e9c
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spelling	oai:doaj.org-article:2c7e2d18f9b541139797761f5a888e9c2021-12-02T11:01:35ZFabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency1176-91141178-2013https://doaj.org/article/2c7e2d18f9b541139797761f5a888e9c2013-06-01T00:00:00Zhttp://www.dovepress.com/fabrication-of-small-diameter-vascular-scaffolds-by-heparin-bonded-pll-a13288https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sheng Wang,1,* Xiu M Mo,2,* Bo J Jiang,1 Cheng J Gao,1 Hong S Wang,2 Yu G Zhuang,1 Li J Qiu21Department of Emergency and Critical Care Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, People's Republic of China; 2State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this workAbstract: The poor patency rate following small-diameter vascular grafting remains a major hurdle for the widespread clinical application of artificial blood vessels to date. Our previous studies found that electrospun poly(L-lactide-co-epsilon-caprolactone) (P[LLA-CL]) nanofibers facilitated the attachment and growth of endothelial cells (EC), and heparin incorporated into P(LLA-CL) nanofibers was able to release in a controlled manner. Hence, we hypothesized that heparin-bonded P(LLA-CL) vascular scaffolds with autologous EC pre-endothelialization could significantly promote the graft patency rate. To construct a small-diameter vascular scaffold, the inner layer was fabricated by heparin-bonded P(LLA-CL) nanofibers through coaxial electrospinning, while the outer layer was woven by pure P(LLA-CL) nanofibers. Except dynamic compliance (5.4 ± 1.7 versus 12.8 ± 2.4 × 10-4/mmHg, P < 0.05), maximal tensile strength, burst pressure, and suture retention of the composite, scaffolds were comparable to those of canine femoral arteries. In vitro studies indicated that the scaffolds can continuously release heparin for at least 12 weeks and obtain desirable endothelialization through dynamic incubation, which was confirmed by EC viability and proliferation assay and scanning electronic microscopy. Furthermore, in vivo studies demonstrated that pre-endothelialization by autologous ECs provided a better effect on graft patency rate in comparison with heparin loading, and the united application of pre-endothelialization and heparin loading markedly promoted the 24 weeks patency rate of P(LLA-CL) scaffolds (88.9% versus 12.5% in the control group, P < 0.05) in the canine femoral artery replacement model. These results suggest that heparin-bonded P(LLA-CL) scaffolds have similar biomechanical properties to those of native arteries and possess a multiporous and biocompatible surface to achieve satisfactory endothelialization in vitro. Heparin-bonded P(LLA-CL) scaffolds with autologous EC pre-endothelialization have the potential to be substitutes for natural small-diameter vessels in planned vascular bypass surgery.Keywords: electrospinning, heparin, vascular graft, endothelialization, patency rateWang SMo XMJiang BJGao CJWang HSZhuang YGQiu LJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2131-2139 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang S Mo XM Jiang BJ Gao CJ Wang HS Zhuang YG Qiu LJ Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
description	Sheng Wang,1,* Xiu M Mo,2,* Bo J Jiang,1 Cheng J Gao,1 Hong S Wang,2 Yu G Zhuang,1 Li J Qiu21Department of Emergency and Critical Care Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, People's Republic of China; 2State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, People’s Republic of China *These authors contributed equally to this workAbstract: The poor patency rate following small-diameter vascular grafting remains a major hurdle for the widespread clinical application of artificial blood vessels to date. Our previous studies found that electrospun poly(L-lactide-co-epsilon-caprolactone) (P[LLA-CL]) nanofibers facilitated the attachment and growth of endothelial cells (EC), and heparin incorporated into P(LLA-CL) nanofibers was able to release in a controlled manner. Hence, we hypothesized that heparin-bonded P(LLA-CL) vascular scaffolds with autologous EC pre-endothelialization could significantly promote the graft patency rate. To construct a small-diameter vascular scaffold, the inner layer was fabricated by heparin-bonded P(LLA-CL) nanofibers through coaxial electrospinning, while the outer layer was woven by pure P(LLA-CL) nanofibers. Except dynamic compliance (5.4 ± 1.7 versus 12.8 ± 2.4 × 10-4/mmHg, P < 0.05), maximal tensile strength, burst pressure, and suture retention of the composite, scaffolds were comparable to those of canine femoral arteries. In vitro studies indicated that the scaffolds can continuously release heparin for at least 12 weeks and obtain desirable endothelialization through dynamic incubation, which was confirmed by EC viability and proliferation assay and scanning electronic microscopy. Furthermore, in vivo studies demonstrated that pre-endothelialization by autologous ECs provided a better effect on graft patency rate in comparison with heparin loading, and the united application of pre-endothelialization and heparin loading markedly promoted the 24 weeks patency rate of P(LLA-CL) scaffolds (88.9% versus 12.5% in the control group, P < 0.05) in the canine femoral artery replacement model. These results suggest that heparin-bonded P(LLA-CL) scaffolds have similar biomechanical properties to those of native arteries and possess a multiporous and biocompatible surface to achieve satisfactory endothelialization in vitro. Heparin-bonded P(LLA-CL) scaffolds with autologous EC pre-endothelialization have the potential to be substitutes for natural small-diameter vessels in planned vascular bypass surgery.Keywords: electrospinning, heparin, vascular graft, endothelialization, patency rate
format	article
author	Wang S Mo XM Jiang BJ Gao CJ Wang HS Zhuang YG Qiu LJ
author_facet	Wang S Mo XM Jiang BJ Gao CJ Wang HS Zhuang YG Qiu LJ
author_sort	Wang S
title	Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
title_short	Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
title_full	Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
title_fullStr	Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
title_full_unstemmed	Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency
title_sort	fabrication of small-diameter vascular scaffolds by heparin-bonded p(lla-cl) composite nanofibers to improve graft patency
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/2c7e2d18f9b541139797761f5a888e9c
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Fabrication of small-diameter vascular scaffolds by heparin-bonded P(LLA-CL) composite nanofibers to improve graft patency

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