Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.

We have investigated the in vitro effects of increased levels of glucose and free fatty acids on autophagy activation in pancreatic beta cells. INS-1E cells and isolated rat and human pancreatic islets were incubated for various times (from 2 to 24 h) at different concentrations of glucose and/or pa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luisa Martino, Matilde Masini, Michela Novelli, Pascale Beffy, Marco Bugliani, Lorella Marselli, Pellegrino Masiello, Piero Marchetti, Vincenzo De Tata
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c7e460aeba2416fb7ed563c023705c1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c7e460aeba2416fb7ed563c023705c1
record_format	dspace
spelling	oai:doaj.org-article:2c7e460aeba2416fb7ed563c023705c12021-11-18T07:20:04ZPalmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.1932-620310.1371/journal.pone.0036188https://doaj.org/article/2c7e460aeba2416fb7ed563c023705c12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22563482/?tool=EBIhttps://doaj.org/toc/1932-6203We have investigated the in vitro effects of increased levels of glucose and free fatty acids on autophagy activation in pancreatic beta cells. INS-1E cells and isolated rat and human pancreatic islets were incubated for various times (from 2 to 24 h) at different concentrations of glucose and/or palmitic acid. Then, cell survival was evaluated and autophagy activation was explored by using various biochemical and morphological techniques. In INS-1E cells as well as in rat and human islets, 0.5 and 1.0 mM palmitate markedly increased autophagic vacuole formation, whereas high glucose was ineffective alone and caused little additional change when combined with palmitate. Furthermore, LC3-II immunofluorescence co-localized with that of cathepsin D, a lysosomal marker, showing that the autophagic flux was not hampered in PA-treated cells. These effects were maintained up to 18-24 h incubation and were associated with a significant decline of cell survival correlated with both palmitate concentration and incubation time. Ultrastructural analysis showed that autophagy activation, as evidenced by the occurrence of many autophagic vacuoles in the cytoplasm of beta cells, was associated with a diffuse and remarkable swelling of the endoplasmic reticulum. Our results indicate that among the metabolic alterations typically associated with type 2 diabetes, high free fatty acids levels could play a role in the activation of autophagy in beta cells, through a mechanism that might involve the induction of endoplasmic reticulum stress.Luisa MartinoMatilde MasiniMichela NovelliPascale BeffyMarco BuglianiLorella MarselliPellegrino MasielloPiero MarchettiVincenzo De TataPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36188 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luisa Martino Matilde Masini Michela Novelli Pascale Beffy Marco Bugliani Lorella Marselli Pellegrino Masiello Piero Marchetti Vincenzo De Tata Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
description	We have investigated the in vitro effects of increased levels of glucose and free fatty acids on autophagy activation in pancreatic beta cells. INS-1E cells and isolated rat and human pancreatic islets were incubated for various times (from 2 to 24 h) at different concentrations of glucose and/or palmitic acid. Then, cell survival was evaluated and autophagy activation was explored by using various biochemical and morphological techniques. In INS-1E cells as well as in rat and human islets, 0.5 and 1.0 mM palmitate markedly increased autophagic vacuole formation, whereas high glucose was ineffective alone and caused little additional change when combined with palmitate. Furthermore, LC3-II immunofluorescence co-localized with that of cathepsin D, a lysosomal marker, showing that the autophagic flux was not hampered in PA-treated cells. These effects were maintained up to 18-24 h incubation and were associated with a significant decline of cell survival correlated with both palmitate concentration and incubation time. Ultrastructural analysis showed that autophagy activation, as evidenced by the occurrence of many autophagic vacuoles in the cytoplasm of beta cells, was associated with a diffuse and remarkable swelling of the endoplasmic reticulum. Our results indicate that among the metabolic alterations typically associated with type 2 diabetes, high free fatty acids levels could play a role in the activation of autophagy in beta cells, through a mechanism that might involve the induction of endoplasmic reticulum stress.
format	article
author	Luisa Martino Matilde Masini Michela Novelli Pascale Beffy Marco Bugliani Lorella Marselli Pellegrino Masiello Piero Marchetti Vincenzo De Tata
author_facet	Luisa Martino Matilde Masini Michela Novelli Pascale Beffy Marco Bugliani Lorella Marselli Pellegrino Masiello Piero Marchetti Vincenzo De Tata
author_sort	Luisa Martino
title	Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
title_short	Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
title_full	Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
title_fullStr	Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
title_full_unstemmed	Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.
title_sort	palmitate activates autophagy in ins-1e β-cells and in isolated rat and human pancreatic islets.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2c7e460aeba2416fb7ed563c023705c1
work_keys_str_mv	AT luisamartino palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT matildemasini palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT michelanovelli palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT pascalebeffy palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT marcobugliani palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT lorellamarselli palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT pellegrinomasiello palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT pieromarchetti palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets AT vincenzodetata palmitateactivatesautophagyinins1ebcellsandinisolatedratandhumanpancreaticislets
_version_	1718423587565600768

Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets.

Ejemplares similares