Characterisation of a tripartite α-pore forming toxin from Serratia marcescens

Abstract Tripartite members of the ClyA family of α-PFTs have recently been identified in a number of pathogenic Gram-negative bacteria, including the human pathogen Serratia marcescens. Structures of a Gram-negative A component and a tripartite α-PFT complete pore are unknown and a mechanism for po...

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Autores principales:	Alicia M. Churchill-Angus, Thomas H. B. Schofield, Thomas R. Marlow, Svetlana E. Sedelnikova, Jason S. Wilson, John B. Rafferty, Patrick J. Baker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c7f359be7964752aa45970e9a0857dc
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spelling	oai:doaj.org-article:2c7f359be7964752aa45970e9a0857dc2021-12-02T17:05:11ZCharacterisation of a tripartite α-pore forming toxin from Serratia marcescens10.1038/s41598-021-85726-02045-2322https://doaj.org/article/2c7f359be7964752aa45970e9a0857dc2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85726-0https://doaj.org/toc/2045-2322Abstract Tripartite members of the ClyA family of α-PFTs have recently been identified in a number of pathogenic Gram-negative bacteria, including the human pathogen Serratia marcescens. Structures of a Gram-negative A component and a tripartite α-PFT complete pore are unknown and a mechanism for pore formation is still uncertain. Here we characterise the tripartite SmhABC toxin from S. marcescens and propose a mechanism of pore assembly. We present the structure of soluble SmhA, as well as the soluble and pore forms of SmhB. We show that the β-tongue soluble structure is well conserved in the family and propose two conserved latches between the head and tail domains that are broken on the soluble to pore conformational change. Using the structures of individual components, sequence analysis and docking predictions we illustrate how the A, B and C protomers would assemble on the membrane to produce a complete tripartite α-PFT pore.Alicia M. Churchill-AngusThomas H. B. SchofieldThomas R. MarlowSvetlana E. SedelnikovaJason S. WilsonJohn B. RaffertyPatrick J. BakerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alicia M. Churchill-Angus Thomas H. B. Schofield Thomas R. Marlow Svetlana E. Sedelnikova Jason S. Wilson John B. Rafferty Patrick J. Baker Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
description	Abstract Tripartite members of the ClyA family of α-PFTs have recently been identified in a number of pathogenic Gram-negative bacteria, including the human pathogen Serratia marcescens. Structures of a Gram-negative A component and a tripartite α-PFT complete pore are unknown and a mechanism for pore formation is still uncertain. Here we characterise the tripartite SmhABC toxin from S. marcescens and propose a mechanism of pore assembly. We present the structure of soluble SmhA, as well as the soluble and pore forms of SmhB. We show that the β-tongue soluble structure is well conserved in the family and propose two conserved latches between the head and tail domains that are broken on the soluble to pore conformational change. Using the structures of individual components, sequence analysis and docking predictions we illustrate how the A, B and C protomers would assemble on the membrane to produce a complete tripartite α-PFT pore.
format	article
author	Alicia M. Churchill-Angus Thomas H. B. Schofield Thomas R. Marlow Svetlana E. Sedelnikova Jason S. Wilson John B. Rafferty Patrick J. Baker
author_facet	Alicia M. Churchill-Angus Thomas H. B. Schofield Thomas R. Marlow Svetlana E. Sedelnikova Jason S. Wilson John B. Rafferty Patrick J. Baker
author_sort	Alicia M. Churchill-Angus
title	Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
title_short	Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
title_full	Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
title_fullStr	Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
title_full_unstemmed	Characterisation of a tripartite α-pore forming toxin from Serratia marcescens
title_sort	characterisation of a tripartite α-pore forming toxin from serratia marcescens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c7f359be7964752aa45970e9a0857dc
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Characterisation of a tripartite α-pore forming toxin from Serratia marcescens

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