Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

Abstract The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase...

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Autores principales:	Shahrbanoo Hamedi, M. Mehdi Afsahi, Ali Riahi-Madvar, Ali Mohebbi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c80be77683344b3ad6ec039e662d400
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spelling	oai:doaj.org-article:2c80be77683344b3ad6ec039e662d4002021-12-02T16:08:07ZModeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer10.1038/s41598-021-93563-42045-2322https://doaj.org/article/2c80be77683344b3ad6ec039e662d4002021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93563-4https://doaj.org/toc/2045-2322Abstract The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.Shahrbanoo HamediM. Mehdi AfsahiAli Riahi-MadvarAli MohebbiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shahrbanoo Hamedi M. Mehdi Afsahi Ali Riahi-Madvar Ali Mohebbi Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
description	Abstract The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.
format	article
author	Shahrbanoo Hamedi M. Mehdi Afsahi Ali Riahi-Madvar Ali Mohebbi
author_facet	Shahrbanoo Hamedi M. Mehdi Afsahi Ali Riahi-Madvar Ali Mohebbi
author_sort	Shahrbanoo Hamedi
title	Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
title_short	Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
title_full	Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
title_fullStr	Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
title_full_unstemmed	Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
title_sort	modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c80be77683344b3ad6ec039e662d400
work_keys_str_mv	AT shahrbanoohamedi modelingandoptimizationofradishrootextractdryingasperoxidasesourceusingspoutedbeddryer AT mmehdiafsahi modelingandoptimizationofradishrootextractdryingasperoxidasesourceusingspoutedbeddryer AT aliriahimadvar modelingandoptimizationofradishrootextractdryingasperoxidasesourceusingspoutedbeddryer AT alimohebbi modelingandoptimizationofradishrootextractdryingasperoxidasesourceusingspoutedbeddryer
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Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

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