Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography

Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesio...

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Autores principales:	Huaiyu Shi, Xiangjun Wu, Shiyang Sun, Chenyan Wang, Zacharias Vangelatos, Ariel Ash-Shakoor, Costas P. Grigoropoulos, Patrick T. Mather, James H. Henderson, Zhen Ma
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Stimuli-responsive biomaterials Shape memory polymer Focal adhesion Costamere Dynamic mechanobiology Human induced pluripotent stem cells Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2c859944a6c8480f852fdd5ab2ccc6ff
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spelling	oai:doaj.org-article:2c859944a6c8480f852fdd5ab2ccc6ff2021-11-28T04:35:18ZProfiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography2452-199X10.1016/j.bioactmat.2021.08.028https://doaj.org/article/2c859944a6c8480f852fdd5ab2ccc6ff2022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21004047https://doaj.org/toc/2452-199XFocal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesions to dynamic surface topographic changes remains underexplored. To study this dynamic responsiveness of focal adhesions, we utilized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature. Using this dynamic culture system, we analyzed three proteins (paxillin, vinculin and zyxin) from different layers of the focal adhesion complex in response to dynamic extracellular topographic change. Hence, we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner, which provides new understanding of dynamic cardiac mechanobiology.Huaiyu ShiXiangjun WuShiyang SunChenyan WangZacharias VangelatosAriel Ash-ShakoorCostas P. GrigoropoulosPatrick T. MatherJames H. HendersonZhen MaKeAi Communications Co., Ltd.articleStimuli-responsive biomaterialsShape memory polymerFocal adhesionCostamereDynamic mechanobiologyHuman induced pluripotent stem cellsMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 367-377 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Stimuli-responsive biomaterials Shape memory polymer Focal adhesion Costamere Dynamic mechanobiology Human induced pluripotent stem cells Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Stimuli-responsive biomaterials Shape memory polymer Focal adhesion Costamere Dynamic mechanobiology Human induced pluripotent stem cells Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Huaiyu Shi Xiangjun Wu Shiyang Sun Chenyan Wang Zacharias Vangelatos Ariel Ash-Shakoor Costas P. Grigoropoulos Patrick T. Mather James H. Henderson Zhen Ma Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
description	Focal adhesion complexes function as the mediators of cell-extracellular matrix interactions to sense and transmit the extracellular signals. Previous studies have demonstrated that cardiomyocyte focal adhesions can be modulated by surface topographic features. However, the response of focal adhesions to dynamic surface topographic changes remains underexplored. To study this dynamic responsiveness of focal adhesions, we utilized a shape memory polymer-based substrate that can produce a flat-to-wrinkle surface transition triggered by an increase of temperature. Using this dynamic culture system, we analyzed three proteins (paxillin, vinculin and zyxin) from different layers of the focal adhesion complex in response to dynamic extracellular topographic change. Hence, we quantified the dynamic profile of cardiomyocyte focal adhesion in a time-dependent manner, which provides new understanding of dynamic cardiac mechanobiology.
format	article
author	Huaiyu Shi Xiangjun Wu Shiyang Sun Chenyan Wang Zacharias Vangelatos Ariel Ash-Shakoor Costas P. Grigoropoulos Patrick T. Mather James H. Henderson Zhen Ma
author_facet	Huaiyu Shi Xiangjun Wu Shiyang Sun Chenyan Wang Zacharias Vangelatos Ariel Ash-Shakoor Costas P. Grigoropoulos Patrick T. Mather James H. Henderson Zhen Ma
author_sort	Huaiyu Shi
title	Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
title_short	Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
title_full	Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
title_fullStr	Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
title_full_unstemmed	Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
title_sort	profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/2c859944a6c8480f852fdd5ab2ccc6ff
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Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography

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