Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients

Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients

Abstract Circulating cell-free DNA (cfDNA) has been investigated as a screening tool for many diseases. To avoid expensive and time-consuming DNA isolation, direct quantification PCR assays can be established. However, rigorous validation is required to provide reliable data in the clinical and non-...

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Autores principales: Elmo W. I. Neuberger, Alexandra Brahmer, Tobias Ehlert, Katrin Kluge, Keito F. A. Philippi, Simone C. Boedecker, Julia Weinmann-Menke, Perikles Simon
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/2c8625c02e284cb487322ebbc35471f4
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