Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients

Abstract Circulating cell-free DNA (cfDNA) has been investigated as a screening tool for many diseases. To avoid expensive and time-consuming DNA isolation, direct quantification PCR assays can be established. However, rigorous validation is required to provide reliable data in the clinical and non-...

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Autores principales: Elmo W. I. Neuberger, Alexandra Brahmer, Tobias Ehlert, Katrin Kluge, Keito F. A. Philippi, Simone C. Boedecker, Julia Weinmann-Menke, Perikles Simon
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/2c8625c02e284cb487322ebbc35471f4
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