LED arrays as cost effective and efficient light sources for widefield microscopy.

New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, diffi...

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Autores principales:	Dinu F Albeanu, Edward Soucy, Tomokazu F Sato, Markus Meister, Venkatesh N Murthy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c8738b4ebea4734a1e6ba4d5c63aba6
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spelling	oai:doaj.org-article:2c8738b4ebea4734a1e6ba4d5c63aba62021-11-25T06:12:30ZLED arrays as cost effective and efficient light sources for widefield microscopy.1932-620310.1371/journal.pone.0002146https://doaj.org/article/2c8738b4ebea4734a1e6ba4d5c63aba62008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18478056/?tool=EBIhttps://doaj.org/toc/1932-6203New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.Dinu F AlbeanuEdward SoucyTomokazu F SatoMarkus MeisterVenkatesh N MurthyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 5, p e2146 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy LED arrays as cost effective and efficient light sources for widefield microscopy.
description	New developments in fluorophores as well as in detection methods have fueled the rapid growth of optical imaging in the life sciences. Commercial widefield microscopes generally use arc lamps, excitation/emission filters and shutters for fluorescence imaging. These components can be expensive, difficult to maintain and preclude stable illumination. Here, we describe methods to construct inexpensive and easy-to-use light sources for optical microscopy using light-emitting diodes (LEDs). We also provide examples of its applicability to biological fluorescence imaging.
format	article
author	Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy
author_facet	Dinu F Albeanu Edward Soucy Tomokazu F Sato Markus Meister Venkatesh N Murthy
author_sort	Dinu F Albeanu
title	LED arrays as cost effective and efficient light sources for widefield microscopy.
title_short	LED arrays as cost effective and efficient light sources for widefield microscopy.
title_full	LED arrays as cost effective and efficient light sources for widefield microscopy.
title_fullStr	LED arrays as cost effective and efficient light sources for widefield microscopy.
title_full_unstemmed	LED arrays as cost effective and efficient light sources for widefield microscopy.
title_sort	led arrays as cost effective and efficient light sources for widefield microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/2c8738b4ebea4734a1e6ba4d5c63aba6
work_keys_str_mv	AT dinufalbeanu ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT edwardsoucy ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT tomokazufsato ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT markusmeister ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy AT venkateshnmurthy ledarraysascosteffectiveandefficientlightsourcesforwidefieldmicroscopy
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LED arrays as cost effective and efficient light sources for widefield microscopy.

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