Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain

The correct reconstruction of individual (crossing) nerve fibers is a prerequisite when constructing a detailed network model of the brain. The recently developed technique Scattered Light Imaging (SLI) allows the reconstruction of crossing nerve fiber pathways in whole brain tissue samples with mic...

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Autores principales:	Miriam Menzel, Marouan Ritzkowski, Jan A. Reuter, David Gräßel, Katrin Amunts, Markus Axer
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	light scattering optical imaging microscopy nerve fiber pathways neuron connectivity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695
Acceso en línea:	https://doaj.org/article/2c89cf9d1b474b9cb12d414e83d16dfd
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spelling	oai:doaj.org-article:2c89cf9d1b474b9cb12d414e83d16dfd2021-12-01T13:22:03ZScatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain1662-512910.3389/fnana.2021.767223https://doaj.org/article/2c89cf9d1b474b9cb12d414e83d16dfd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnana.2021.767223/fullhttps://doaj.org/toc/1662-5129The correct reconstruction of individual (crossing) nerve fibers is a prerequisite when constructing a detailed network model of the brain. The recently developed technique Scattered Light Imaging (SLI) allows the reconstruction of crossing nerve fiber pathways in whole brain tissue samples with micrometer resolution: the individual fiber orientations are determined by illuminating unstained histological brain sections from different directions, measuring the transmitted scattered light under normal incidence, and studying the light intensity profiles of each pixel in the resulting image series. So far, SLI measurements were performed with a fixed polar angle of illumination and a small number of illumination directions, providing only an estimate of the nerve fiber directions and limited information about the underlying tissue structure. Here, we use a display with individually controllable light-emitting diodes to measure the full distribution of scattered light behind the sample (scattering pattern) for each image pixel at once, enabling scatterometry measurements of whole brain tissue samples. We compare our results to coherent Fourier scatterometry (raster-scanning the sample with a non-focused laser beam) and previous SLI measurements with fixed polar angle of illumination, using sections from a vervet monkey brain and human optic tracts. Finally, we present SLI scatterometry measurements of a human brain section with 3 μm in-plane resolution, demonstrating that the technique is a powerful approach to gain new insights into the nerve fiber architecture of the human brain.Miriam MenzelMarouan RitzkowskiJan A. ReuterDavid GräßelKatrin AmuntsKatrin AmuntsMarkus AxerFrontiers Media S.A.articlelight scatteringoptical imagingmicroscopynerve fiber pathwaysneuronconnectivityNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Human anatomyQM1-695ENFrontiers in Neuroanatomy, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	light scattering optical imaging microscopy nerve fiber pathways neuron connectivity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695
spellingShingle	light scattering optical imaging microscopy nerve fiber pathways neuron connectivity Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Human anatomy QM1-695 Miriam Menzel Marouan Ritzkowski Jan A. Reuter David Gräßel Katrin Amunts Katrin Amunts Markus Axer Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
description	The correct reconstruction of individual (crossing) nerve fibers is a prerequisite when constructing a detailed network model of the brain. The recently developed technique Scattered Light Imaging (SLI) allows the reconstruction of crossing nerve fiber pathways in whole brain tissue samples with micrometer resolution: the individual fiber orientations are determined by illuminating unstained histological brain sections from different directions, measuring the transmitted scattered light under normal incidence, and studying the light intensity profiles of each pixel in the resulting image series. So far, SLI measurements were performed with a fixed polar angle of illumination and a small number of illumination directions, providing only an estimate of the nerve fiber directions and limited information about the underlying tissue structure. Here, we use a display with individually controllable light-emitting diodes to measure the full distribution of scattered light behind the sample (scattering pattern) for each image pixel at once, enabling scatterometry measurements of whole brain tissue samples. We compare our results to coherent Fourier scatterometry (raster-scanning the sample with a non-focused laser beam) and previous SLI measurements with fixed polar angle of illumination, using sections from a vervet monkey brain and human optic tracts. Finally, we present SLI scatterometry measurements of a human brain section with 3 μm in-plane resolution, demonstrating that the technique is a powerful approach to gain new insights into the nerve fiber architecture of the human brain.
format	article
author	Miriam Menzel Marouan Ritzkowski Jan A. Reuter David Gräßel Katrin Amunts Katrin Amunts Markus Axer
author_facet	Miriam Menzel Marouan Ritzkowski Jan A. Reuter David Gräßel Katrin Amunts Katrin Amunts Markus Axer
author_sort	Miriam Menzel
title	Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
title_short	Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
title_full	Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
title_fullStr	Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
title_full_unstemmed	Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain
title_sort	scatterometry measurements with scattered light imaging enable new insights into the nerve fiber architecture of the brain
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2c89cf9d1b474b9cb12d414e83d16dfd
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_version_	1718405108850491392

Scatterometry Measurements With Scattered Light Imaging Enable New Insights Into the Nerve Fiber Architecture of the Brain

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