In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines

The study describes the cytotoxic effect against human and canine osteosarcoma (U-2 OS and D-17) cell lines induced by risedronate sodium and meloxicam per se and in combination. Both cell lines were prepared according to standard procedures for cell cultures studies. The cell viability was estimate...

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Autores principales:	Dominik Poradowski, Izabela Janus, Aleksander Chrószcz, Bożena Obmińska-Mrukowicz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	osteosarcoma human canine risedronate sodium meloxicam interaction Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/2c8ac1ebb57f488a8bd099c28328b010
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spelling	oai:doaj.org-article:2c8ac1ebb57f488a8bd099c28328b0102021-11-25T16:16:34ZIn Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines10.3390/ani111131352076-2615https://doaj.org/article/2c8ac1ebb57f488a8bd099c28328b0102021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3135https://doaj.org/toc/2076-2615The study describes the cytotoxic effect against human and canine osteosarcoma (U-2 OS and D-17) cell lines induced by risedronate sodium and meloxicam per se and in combination. Both cell lines were prepared according to standard procedures for cell cultures studies. The cell viability was estimated in both cell lines treated with chosen concentrations of risedronate sodium and meloxicam. The apoptosis assessment was carried out using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay. EC<sub>50</sub> values, computed for risedronate sodium and meloxicam cytotoxicity, showed comparable effects against the canine OS cell line in similar concentration of both drugs. In case of human OS, the stronger cytotoxic effect of risedronate sodium was proved. The EC<sub>50</sub> values for meloxicam in both cell lines were, statistically, significantly different (* <i>p</i> < 0.05). Moreover, the cytotoxic effect of a combined administration of meloxicam and risedronate sodium in doses 100 µg/mL, compared with the negative control showed statistically significant differences. The human OS cell line was more resistant to both compounds than the canine OS cell line. The apoptotic effect in canine and human osteosarcoma triggered by risedronate sodium and meloxicam was statistically significant (<i>p</i> < 0.05). The cytotoxic effect induced with 100 µg/mL of risedronate sodium proved statistically significant differences between both tested cell lines compared to negative control. The results obtained with 10 and 100 µg/mL of meloxicam were not statistically significant. The study showed the synergic mechanism of action of risedronate sodium and meloxicam, but the concentrations used in vitro will not be possible to achieve in in vivo. Therefore, our results serve as basis only to design future studies on the tissue level.Dominik PoradowskiIzabela JanusAleksander ChrószczBożena Obmińska-MrukowiczMDPI AGarticleosteosarcomahumancaninerisedronate sodiummeloxicaminteractionVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3135, p 3135 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteosarcoma human canine risedronate sodium meloxicam interaction Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	osteosarcoma human canine risedronate sodium meloxicam interaction Veterinary medicine SF600-1100 Zoology QL1-991 Dominik Poradowski Izabela Janus Aleksander Chrószcz Bożena Obmińska-Mrukowicz In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
description	The study describes the cytotoxic effect against human and canine osteosarcoma (U-2 OS and D-17) cell lines induced by risedronate sodium and meloxicam per se and in combination. Both cell lines were prepared according to standard procedures for cell cultures studies. The cell viability was estimated in both cell lines treated with chosen concentrations of risedronate sodium and meloxicam. The apoptosis assessment was carried out using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay. EC<sub>50</sub> values, computed for risedronate sodium and meloxicam cytotoxicity, showed comparable effects against the canine OS cell line in similar concentration of both drugs. In case of human OS, the stronger cytotoxic effect of risedronate sodium was proved. The EC<sub>50</sub> values for meloxicam in both cell lines were, statistically, significantly different (* <i>p</i> < 0.05). Moreover, the cytotoxic effect of a combined administration of meloxicam and risedronate sodium in doses 100 µg/mL, compared with the negative control showed statistically significant differences. The human OS cell line was more resistant to both compounds than the canine OS cell line. The apoptotic effect in canine and human osteosarcoma triggered by risedronate sodium and meloxicam was statistically significant (<i>p</i> < 0.05). The cytotoxic effect induced with 100 µg/mL of risedronate sodium proved statistically significant differences between both tested cell lines compared to negative control. The results obtained with 10 and 100 µg/mL of meloxicam were not statistically significant. The study showed the synergic mechanism of action of risedronate sodium and meloxicam, but the concentrations used in vitro will not be possible to achieve in in vivo. Therefore, our results serve as basis only to design future studies on the tissue level.
format	article
author	Dominik Poradowski Izabela Janus Aleksander Chrószcz Bożena Obmińska-Mrukowicz
author_facet	Dominik Poradowski Izabela Janus Aleksander Chrószcz Bożena Obmińska-Mrukowicz
author_sort	Dominik Poradowski
title	In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
title_short	In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
title_full	In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
title_fullStr	In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
title_full_unstemmed	In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines
title_sort	in vitro studies on the influence of meloxicam on cytotoxic activity induced by risedronate sodium in canine (d-17) and human (u-2 os) osteosarcoma cell lines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2c8ac1ebb57f488a8bd099c28328b010
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In Vitro Studies on the Influence of Meloxicam on Cytotoxic Activity Induced by Risedronate Sodium in Canine (D-17) and Human (U-2 OS) Osteosarcoma Cell Lines

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