Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix

Acellular adipose matrix (AAM) has emerged as an important biomaterial for adipose tissue regeneration. Current decellularization methods damage the bioactive components of the extracellular matrix (ECM), and the residual immunogenic antigens may induce adverse immune responses. Here, we adopted a m...

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Autores principales:	Kaiyang Liu, Yunfan He, Yao Yao, Yuchen Zhang, Zihan Cai, Jiangjiang Ru, Xiangdong Zhang, Xiaoxuan Jin, Mimi Xu, Yibao Li, Qizhuan Ma, Jianhua Gao, Feng Lu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Acellular adipose matrix Xenotransplantation Methoxy polyethylene glycol Regulatory T cell Adipogenesis Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2c8cd3a028324b2c9ae4bffac061202e
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spelling	oai:doaj.org-article:2c8cd3a028324b2c9ae4bffac061202e2021-11-24T04:33:52ZMethoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix2590-006410.1016/j.mtbio.2021.100161https://doaj.org/article/2c8cd3a028324b2c9ae4bffac061202e2021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000697https://doaj.org/toc/2590-0064Acellular adipose matrix (AAM) has emerged as an important biomaterial for adipose tissue regeneration. Current decellularization methods damage the bioactive components of the extracellular matrix (ECM), and the residual immunogenic antigens may induce adverse immune responses. Here, we adopted a modified decellularization method which can protect more bioactive components with less immune reaction by methoxy polyethylene glycol (mPEG). Then, we determined the adipogenic mechanisms of mPEG-modified AAM after xenogeneic transplantation. AAM transplantation caused significantly lesser adipogenesis in the wild-type group than in the immune-deficient group. The mPEG-modified AAM showed significantly lower immunogenicity and higher adipogenesis than the AAM alone after xenogeneic transplantation. Furthermore, mPEG modification increased regulatory T (Treg) cell numbers in the AAM grafts, which in turn enhanced the M2/M1 macrophage ratio by secreting IL-10, IL-13, and TGF-β1. These findings suggest that mPEG modification effectively reduces the immunogenicity of xenogeneic AAM and promotes adipogenesis in the AAM grafts. Hence, mPEG-modified AAM can serve as an ideal biomaterial for xenogeneic adipose tissue engineering.Kaiyang LiuYunfan HeYao YaoYuchen ZhangZihan CaiJiangjiang RuXiangdong ZhangXiaoxuan JinMimi XuYibao LiQizhuan MaJianhua GaoFeng LuElsevierarticleAcellular adipose matrixXenotransplantationMethoxy polyethylene glycolRegulatory T cellAdipogenesisMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100161- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acellular adipose matrix Xenotransplantation Methoxy polyethylene glycol Regulatory T cell Adipogenesis Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Acellular adipose matrix Xenotransplantation Methoxy polyethylene glycol Regulatory T cell Adipogenesis Medicine (General) R5-920 Biology (General) QH301-705.5 Kaiyang Liu Yunfan He Yao Yao Yuchen Zhang Zihan Cai Jiangjiang Ru Xiangdong Zhang Xiaoxuan Jin Mimi Xu Yibao Li Qizhuan Ma Jianhua Gao Feng Lu Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
description	Acellular adipose matrix (AAM) has emerged as an important biomaterial for adipose tissue regeneration. Current decellularization methods damage the bioactive components of the extracellular matrix (ECM), and the residual immunogenic antigens may induce adverse immune responses. Here, we adopted a modified decellularization method which can protect more bioactive components with less immune reaction by methoxy polyethylene glycol (mPEG). Then, we determined the adipogenic mechanisms of mPEG-modified AAM after xenogeneic transplantation. AAM transplantation caused significantly lesser adipogenesis in the wild-type group than in the immune-deficient group. The mPEG-modified AAM showed significantly lower immunogenicity and higher adipogenesis than the AAM alone after xenogeneic transplantation. Furthermore, mPEG modification increased regulatory T (Treg) cell numbers in the AAM grafts, which in turn enhanced the M2/M1 macrophage ratio by secreting IL-10, IL-13, and TGF-β1. These findings suggest that mPEG modification effectively reduces the immunogenicity of xenogeneic AAM and promotes adipogenesis in the AAM grafts. Hence, mPEG-modified AAM can serve as an ideal biomaterial for xenogeneic adipose tissue engineering.
format	article
author	Kaiyang Liu Yunfan He Yao Yao Yuchen Zhang Zihan Cai Jiangjiang Ru Xiangdong Zhang Xiaoxuan Jin Mimi Xu Yibao Li Qizhuan Ma Jianhua Gao Feng Lu
author_facet	Kaiyang Liu Yunfan He Yao Yao Yuchen Zhang Zihan Cai Jiangjiang Ru Xiangdong Zhang Xiaoxuan Jin Mimi Xu Yibao Li Qizhuan Ma Jianhua Gao Feng Lu
author_sort	Kaiyang Liu
title	Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
title_short	Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
title_full	Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
title_fullStr	Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
title_full_unstemmed	Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix
title_sort	methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory t cells in xenogeneic acellular adipose matrix
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2c8cd3a028324b2c9ae4bffac061202e
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Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix

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