A glance at the gut microbiota of five experimental animal species through fecal samples

Abstract Experimental animals including the ferret, marmoset, woodchuck, mini pig, and tree shrew have been used in biomedical research. However, their gut microbiota have not been fully investigated. In this study, the gut microbiota of these five experimental animals were analyzed with 16S rRNA se...

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Autores principales:	Zhiguang Xiang, Hua Zhu, Bochao Yang, Hang Fan, Jianguo Guo, Jiangning Liu, Qi Kong, Qingfeng Teng, Haiquan Shang, Lei Su, Chuan Qin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c912b177c7f493797e30393b1366cd2
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spelling	oai:doaj.org-article:2c912b177c7f493797e30393b1366cd22021-12-02T18:37:07ZA glance at the gut microbiota of five experimental animal species through fecal samples10.1038/s41598-020-73985-22045-2322https://doaj.org/article/2c912b177c7f493797e30393b1366cd22020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73985-2https://doaj.org/toc/2045-2322Abstract Experimental animals including the ferret, marmoset, woodchuck, mini pig, and tree shrew have been used in biomedical research. However, their gut microbiota have not been fully investigated. In this study, the gut microbiota of these five experimental animals were analyzed with 16S rRNA sequencing. The phyla Firmicutes, Bacteroidetes, and Fusobacteria were present in the gut microbiota of all the species. Specific phyla were present in different animals: Proteobacteria in the ferret, Tenericutes in the marmoset, and Spirochaetes in the mini pig. Fusobacterium and unidentified Clostridiales were the dominant genera in the ferret, whereas Libanicoccus, Lactobacillus, Porphyromonas, and Peptoclostridium were specific to marmoset, mini pig, woodchuck, and tree shrew, respectively. A clustering analysis showed that the overall distribution of microbial species in the guts of these species mirrored their mammalian phylogeny, and the microbiota of the marmoset and tree shrew showed the closest bray_curtis distances to that of humans. PICRUSt functional prediction separated the woodchuck from the other species, which may reflect its herbivorous diet. In conclusion, both the evolutionary phylogeny and daily diet affect the gut microbiota of these experimental animals, which should not be neglected for their usage in biomedical research.Zhiguang XiangHua ZhuBochao YangHang FanJianguo GuoJiangning LiuQi KongQingfeng TengHaiquan ShangLei SuChuan QinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiguang Xiang Hua Zhu Bochao Yang Hang Fan Jianguo Guo Jiangning Liu Qi Kong Qingfeng Teng Haiquan Shang Lei Su Chuan Qin A glance at the gut microbiota of five experimental animal species through fecal samples
description	Abstract Experimental animals including the ferret, marmoset, woodchuck, mini pig, and tree shrew have been used in biomedical research. However, their gut microbiota have not been fully investigated. In this study, the gut microbiota of these five experimental animals were analyzed with 16S rRNA sequencing. The phyla Firmicutes, Bacteroidetes, and Fusobacteria were present in the gut microbiota of all the species. Specific phyla were present in different animals: Proteobacteria in the ferret, Tenericutes in the marmoset, and Spirochaetes in the mini pig. Fusobacterium and unidentified Clostridiales were the dominant genera in the ferret, whereas Libanicoccus, Lactobacillus, Porphyromonas, and Peptoclostridium were specific to marmoset, mini pig, woodchuck, and tree shrew, respectively. A clustering analysis showed that the overall distribution of microbial species in the guts of these species mirrored their mammalian phylogeny, and the microbiota of the marmoset and tree shrew showed the closest bray_curtis distances to that of humans. PICRUSt functional prediction separated the woodchuck from the other species, which may reflect its herbivorous diet. In conclusion, both the evolutionary phylogeny and daily diet affect the gut microbiota of these experimental animals, which should not be neglected for their usage in biomedical research.
format	article
author	Zhiguang Xiang Hua Zhu Bochao Yang Hang Fan Jianguo Guo Jiangning Liu Qi Kong Qingfeng Teng Haiquan Shang Lei Su Chuan Qin
author_facet	Zhiguang Xiang Hua Zhu Bochao Yang Hang Fan Jianguo Guo Jiangning Liu Qi Kong Qingfeng Teng Haiquan Shang Lei Su Chuan Qin
author_sort	Zhiguang Xiang
title	A glance at the gut microbiota of five experimental animal species through fecal samples
title_short	A glance at the gut microbiota of five experimental animal species through fecal samples
title_full	A glance at the gut microbiota of five experimental animal species through fecal samples
title_fullStr	A glance at the gut microbiota of five experimental animal species through fecal samples
title_full_unstemmed	A glance at the gut microbiota of five experimental animal species through fecal samples
title_sort	glance at the gut microbiota of five experimental animal species through fecal samples
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2c912b177c7f493797e30393b1366cd2
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A glance at the gut microbiota of five experimental animal species through fecal samples

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