RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium

Abstract Calcium (Ca) is required for normal growth and is involved in cellular physiology, signal transduction, and bone mineralization. In humans, inadequate Ca intake causes hypocalcaemia, and excessive Ca intake causes hypercalcemia. In chicken, Ca is also required for body weight gain and eggsh...

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Autores principales:	Woncheoul Park, Deivendran Rengaraj, Dong-Yong Kil, Heebal Kim, Hak-Kyo Lee, Ki-Duk Song
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c94f90e6f034ee3a719d150c5b6ac65
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spelling	oai:doaj.org-article:2c94f90e6f034ee3a719d150c5b6ac652021-12-02T11:40:41ZRNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium10.1038/s41598-017-11379-72045-2322https://doaj.org/article/2c94f90e6f034ee3a719d150c5b6ac652017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11379-7https://doaj.org/toc/2045-2322Abstract Calcium (Ca) is required for normal growth and is involved in cellular physiology, signal transduction, and bone mineralization. In humans, inadequate Ca intake causes hypocalcaemia, and excessive Ca intake causes hypercalcemia. In chicken, Ca is also required for body weight gain and eggshell formation. However, transcriptomic responses to low/high Ca intake, and mechanisms affecting body weight have not been explored. In this study, we performed comparative RNA sequencing (RNA-seq) using the kidney of broiler chickens fed diets containing 0.8, 1.0, and 1.2% Ca. Annotation of RNA-seq data revealed a significant number of differentially expressed genes (DEGs) in the kidney via pairwise comparison using Cufflinks and edgeR. Using edgeR, we identified 12 DEGs; seven overlapped with those found by cufflinks. Seven DEGs were validated by real-time quantitative-PCR (qRT-PCR) in Ca-supplemented kidneys, and the results correlated with the RNA-seq data. DEGs identified by cufflinks/edgeR were subjected to pathway enrichment, protein/protein interaction, and co-occurrence analyses to determine their involvement in disease. The National Research Council (NRC) recommended Ca intake for 21-day post-hatch broilers is about 1.0%. Our findings suggest that higher-than-recommended Ca intake (1.2%) could reduce body weight gain in broilers, and that affected DEGs are related to stress-induced diseases, such as hypertension.Woncheoul ParkDeivendran RengarajDong-Yong KilHeebal KimHak-Kyo LeeKi-Duk SongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Woncheoul Park Deivendran Rengaraj Dong-Yong Kil Heebal Kim Hak-Kyo Lee Ki-Duk Song RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
description	Abstract Calcium (Ca) is required for normal growth and is involved in cellular physiology, signal transduction, and bone mineralization. In humans, inadequate Ca intake causes hypocalcaemia, and excessive Ca intake causes hypercalcemia. In chicken, Ca is also required for body weight gain and eggshell formation. However, transcriptomic responses to low/high Ca intake, and mechanisms affecting body weight have not been explored. In this study, we performed comparative RNA sequencing (RNA-seq) using the kidney of broiler chickens fed diets containing 0.8, 1.0, and 1.2% Ca. Annotation of RNA-seq data revealed a significant number of differentially expressed genes (DEGs) in the kidney via pairwise comparison using Cufflinks and edgeR. Using edgeR, we identified 12 DEGs; seven overlapped with those found by cufflinks. Seven DEGs were validated by real-time quantitative-PCR (qRT-PCR) in Ca-supplemented kidneys, and the results correlated with the RNA-seq data. DEGs identified by cufflinks/edgeR were subjected to pathway enrichment, protein/protein interaction, and co-occurrence analyses to determine their involvement in disease. The National Research Council (NRC) recommended Ca intake for 21-day post-hatch broilers is about 1.0%. Our findings suggest that higher-than-recommended Ca intake (1.2%) could reduce body weight gain in broilers, and that affected DEGs are related to stress-induced diseases, such as hypertension.
format	article
author	Woncheoul Park Deivendran Rengaraj Dong-Yong Kil Heebal Kim Hak-Kyo Lee Ki-Duk Song
author_facet	Woncheoul Park Deivendran Rengaraj Dong-Yong Kil Heebal Kim Hak-Kyo Lee Ki-Duk Song
author_sort	Woncheoul Park
title	RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
title_short	RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
title_full	RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
title_fullStr	RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
title_full_unstemmed	RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
title_sort	rna-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2c94f90e6f034ee3a719d150c5b6ac65
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RNA-seq analysis of the kidneys of broiler chickens fed diets containing different concentrations of calcium

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