Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry

Abstract Many reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated...

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Autores principales:	Lue Sun, Yohei Inaba, Keizo Sato, Aki Hirayama, Koji Tsuboi, Ryuji Okazaki, Koichi Chida, Takashi Moritake
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c953a9e773d403787bbdcba4f5f8400
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spelling	oai:doaj.org-article:2c953a9e773d403787bbdcba4f5f84002021-12-02T16:08:02ZDose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry10.1038/s41598-018-25650-y2045-2322https://doaj.org/article/2c953a9e773d403787bbdcba4f5f84002018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25650-yhttps://doaj.org/toc/2045-2322Abstract Many reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated animals and humans. Blood redox homeostasis reflects the body condition well and can be used as a diagnostic marker. However, most redox homeostasis studies have focused on plasma or serum, and the anti-oxidant capacity of whole blood has scarcely been investigated. Here, we report changes in the anti-oxidant capacity of whole blood after X-ray irradiation using C57BL/6 J mice. Whole-blood anti-oxidant capacity was measured by electron spin resonance (ESR) spin trapping using a novel spin-trapping agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO). We found that whole-blood anti-oxidant capacity decreased in a dose-dependent manner (correlation factor, r > 0.9; P < 0.05) from 2 to 24 days after irradiation with 0.5–3 Gy. We further found that the red blood cell (RBC) glutathione level decreased and lipid peroxidation level increased in a dose-dependent manner from 2 to 6 days after irradiation. These findings suggest that blood redox state may be a useful biomarker for estimating exposure doses during nuclear and/or radiation accidents.Lue SunYohei InabaKeizo SatoAki HirayamaKoji TsuboiRyuji OkazakiKoichi ChidaTakashi MoritakeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lue Sun Yohei Inaba Keizo Sato Aki Hirayama Koji Tsuboi Ryuji Okazaki Koichi Chida Takashi Moritake Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
description	Abstract Many reports have demonstrated that radiation stimulates reactive oxygen species (ROS) production by mitochondria for a few hours to a few days after irradiation. However, these studies were performed using cell lines, and there is a lack of information about redox homeostasis in irradiated animals and humans. Blood redox homeostasis reflects the body condition well and can be used as a diagnostic marker. However, most redox homeostasis studies have focused on plasma or serum, and the anti-oxidant capacity of whole blood has scarcely been investigated. Here, we report changes in the anti-oxidant capacity of whole blood after X-ray irradiation using C57BL/6 J mice. Whole-blood anti-oxidant capacity was measured by electron spin resonance (ESR) spin trapping using a novel spin-trapping agent, 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO). We found that whole-blood anti-oxidant capacity decreased in a dose-dependent manner (correlation factor, r > 0.9; P < 0.05) from 2 to 24 days after irradiation with 0.5–3 Gy. We further found that the red blood cell (RBC) glutathione level decreased and lipid peroxidation level increased in a dose-dependent manner from 2 to 6 days after irradiation. These findings suggest that blood redox state may be a useful biomarker for estimating exposure doses during nuclear and/or radiation accidents.
format	article
author	Lue Sun Yohei Inaba Keizo Sato Aki Hirayama Koji Tsuboi Ryuji Okazaki Koichi Chida Takashi Moritake
author_facet	Lue Sun Yohei Inaba Keizo Sato Aki Hirayama Koji Tsuboi Ryuji Okazaki Koichi Chida Takashi Moritake
author_sort	Lue Sun
title	Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
title_short	Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
title_full	Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
title_fullStr	Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
title_full_unstemmed	Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry
title_sort	dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: a novel potential marker for biodosimetry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/2c953a9e773d403787bbdcba4f5f8400
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Dose-dependent decrease in anti-oxidant capacity of whole blood after irradiation: A novel potential marker for biodosimetry

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