Effects of Taohong Siwu Decoction on coagulation function and PI3K/ AKT/mTOR pathway after femoral artery anastomosis in rabbits
Objective: To observe the effect of Taohong Siyu Decoction on the coagulation function and the signaling pathway of PI3K (phosphatidylinositol 3-kinase) /AKT (protein kinase B) /mTOR (mammalian target of rapamycin) after femoral artery anastomosis in rabbits. Methods: 30 New Zealand white rabbits...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/2c99952f05a946b8b59f16e343af25bf |
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| Sumario: | Objective: To observe the effect of Taohong Siyu Decoction on the coagulation function
and the signaling pathway of PI3K (phosphatidylinositol 3-kinase) /AKT (protein kinase
B) /mTOR (mammalian target of rapamycin) after femoral artery anastomosis in rabbits.
Methods: 30 New Zealand white rabbits were divided into blank control group,model control
group,papavine hydrochloride injection group and low, medium and high dose groups of
Taohong Siwu decoction by random number table method, with 5 rabbits in each group. The
rabbits in the model control group,papavine hydrochloride injection group and low, medium
and high dose groups of Taohong Siwu decoction were treated with the femoral artery simple
intermittent end-to-end suture model. After the successful modeling,the low, medium and
high dose groups of Taohong Siwu decoction were given the Taohong Siwu decoction, while
the model control group,the blank control group and papavine hydrochloride injection group
were given the same amount of normal saline.APTT (activated partial thromboplastin time),
FIB (fibrinogen) and PI3K/AKT/mTOR concentrations were measured in aural venous
blood samples from six groups of rabbits 30min before operation and 1d, 2D, 3D and 7d
after operation, respectively.Statistical analysis was conducted on the data of the six groups.
Results: Compared with blank control group,APTT of model control group was significantly
shortened 1d to 7d after operation(P<0.05),FIB values were significantly increased from
1d to 7d after operation(P<0.05);Compared with model control group, APTT in Taohong
Siwu decoction low-dose, medium-dose and high-dose groups were significantly prolonged
1d to 7d after operation(P<0.05),FIB value of Taohong Siwu decoction medium and high
dose groups decreased significantly from 1d to 7d after operation (P<0.05), the FIB value
of Taohong Siwu decoction low-dose group was significantly decreased from 2d to 7d after
surgery (P<0.05);Compared with papaverine hydrochloride injection group,APTT in Taohong
Siwu decoction medium dose group was significantly prolonged 2d to 7d after surgery
(P<0.05),APTT of Taohong Siwu decoction high-dose group was significantly prolonged on 1d
to 7d after operation (P<0.05).FIB in Taohong Siwu decoction medium and high dose groups
decreased significantly 1d to 7d after operation (P<0.05);Compared with the blank control
group, the expression concentrations of PI3K, Akt and mTOR in serum of the model control
group were significantly increased from 1d to 7d after surgery (P<0.05);Compared with the
model control group, the expression levels of PI3K, Akt and mTOR in serum were significantly
increased in the low dose group of Taohong Siwu decoction and Papaverine Hydrochloride
Injection group on postoperative 7 days (P<0.05), Taohong Siwu decoction high-dose group
was significantly increased from 1d to 7d after surgery (P<0.05),the expression concentrations
of PI3K and Akt in Taohong Siwu decoction medium dose group were significantly increased
from 2d to 7d after operation (P<0.05), mTOR expression levels were significantly increased
from 3d to 7d after operation (P<0.05); Compared with papaverine hydrochloride injection
group, the expression concentrations of PI3K, Akt and mTOR in serum of Taohong Siwu
decoction medium dose group were significantly increased from 3d to 7d after operation
(P<0.05), the expression concentrations of PI3K and mTOR in Taohong Siwu decoction
high-dose group were significantly increased from 1d to 7d after operation (P<0.05), and the
expression concentration of Akt increased significantly from 3d to 7d after operation (P<0.05).
Conclusion: The Taohong Siwu decoction can improve the coagulation function of rabbit
femoral artery anastomosis, prevent thrombosis, activate PI3K/Akt/mTOR signaling pathway,
promote angiogenesis, and improve tissue ischemia after artery anastomosis. |
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