Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys

Background: Ultrasound is ideal for displaying intracranial great vessels but not intracranial microvessels and terminal vessels. Even with contrast agents, the imaging effect is still unsatisfactory. In recent years, significant theoretical advances have been achieved in super-resolution imaging. T...

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Autores principales:	Li Yan, Chen Bai, Yu Zheng, Xiaodong Zhou, Mingxi Wan, Yujin Zong, Shanshan Chen, Yin Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	super-resolution imaging ultrasound cerebral vessel microbubbles monkey Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/2c9d9f13430d49309bac277ca40aa41b
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spelling	oai:doaj.org-article:2c9d9f13430d49309bac277ca40aa41b2021-11-17T04:27:30ZStudy on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys1664-229510.3389/fneur.2021.720320https://doaj.org/article/2c9d9f13430d49309bac277ca40aa41b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.720320/fullhttps://doaj.org/toc/1664-2295Background: Ultrasound is ideal for displaying intracranial great vessels but not intracranial microvessels and terminal vessels. Even with contrast agents, the imaging effect is still unsatisfactory. In recent years, significant theoretical advances have been achieved in super-resolution imaging. The latest commonly used ultrafast plane-wave ultrasound Doppler imaging of the brain and microbubble-based super-resolution ultrasound imaging have been applied to the imaging of cerebral microvessels and blood flow in small animals such as mice but have not been applied to in vivo imaging of the cerebral microvessels in monkeys and larger animals. In China, preliminary research results have been obtained using super-resolution imaging in certain fields but rarely in fundamental and clinical experiments on large animals. In recent years, we have conducted a joint study with the Xi'an Jiaotong University to explore the application and performance of this new technique in the diagnosis of cerebrovascular diseases in large animals.Objective: To explore the characteristics and advantages of microbubble-based super-resolution ultrasound imaging of intracranial vessels in rhesus monkeys compared with conventional transcranial ultrasound.Methods: First, the effectiveness and feasibility of the super-resolution imaging technique were verified by modular simulation experiments. Then, the imaging parameters were adjusted based on in vitro experiments. Finally, two rhesus monkeys were used for in vivo experiments of intracranial microvessel imaging.Results: Compared with conventional plane-wave imaging, super-resolution imaging could measure the inner diameters of cerebral microvessels at a resolution of 1 mm or even 0.7 mm and extract blood flow information. In addition, it has a better signal-to-noise ratio (5.625 dB higher) and higher resolution (~30-fold higher). The results of the experiments with rhesus monkeys showed that microbubble-based super-resolution ultrasound imaging can achieve an optimal resolution at the micron level and an imaging depth >35 mm.Conclusion: Super-resolution imaging can realize the monitoring imaging of high-resolution and fast calculation of microbubbles in the process of tissue damage, providing an important experimental basis for the clinical application of non-invasive transcranial ultrasound.Li YanChen BaiYu ZhengXiaodong ZhouMingxi WanYujin ZongShanshan ChenYin ZhouFrontiers Media S.A.articlesuper-resolution imagingultrasoundcerebral vesselmicrobubblesmonkeyNeurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	super-resolution imaging ultrasound cerebral vessel microbubbles monkey Neurology. Diseases of the nervous system RC346-429
spellingShingle	super-resolution imaging ultrasound cerebral vessel microbubbles monkey Neurology. Diseases of the nervous system RC346-429 Li Yan Chen Bai Yu Zheng Xiaodong Zhou Mingxi Wan Yujin Zong Shanshan Chen Yin Zhou Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
description	Background: Ultrasound is ideal for displaying intracranial great vessels but not intracranial microvessels and terminal vessels. Even with contrast agents, the imaging effect is still unsatisfactory. In recent years, significant theoretical advances have been achieved in super-resolution imaging. The latest commonly used ultrafast plane-wave ultrasound Doppler imaging of the brain and microbubble-based super-resolution ultrasound imaging have been applied to the imaging of cerebral microvessels and blood flow in small animals such as mice but have not been applied to in vivo imaging of the cerebral microvessels in monkeys and larger animals. In China, preliminary research results have been obtained using super-resolution imaging in certain fields but rarely in fundamental and clinical experiments on large animals. In recent years, we have conducted a joint study with the Xi'an Jiaotong University to explore the application and performance of this new technique in the diagnosis of cerebrovascular diseases in large animals.Objective: To explore the characteristics and advantages of microbubble-based super-resolution ultrasound imaging of intracranial vessels in rhesus monkeys compared with conventional transcranial ultrasound.Methods: First, the effectiveness and feasibility of the super-resolution imaging technique were verified by modular simulation experiments. Then, the imaging parameters were adjusted based on in vitro experiments. Finally, two rhesus monkeys were used for in vivo experiments of intracranial microvessel imaging.Results: Compared with conventional plane-wave imaging, super-resolution imaging could measure the inner diameters of cerebral microvessels at a resolution of 1 mm or even 0.7 mm and extract blood flow information. In addition, it has a better signal-to-noise ratio (5.625 dB higher) and higher resolution (~30-fold higher). The results of the experiments with rhesus monkeys showed that microbubble-based super-resolution ultrasound imaging can achieve an optimal resolution at the micron level and an imaging depth >35 mm.Conclusion: Super-resolution imaging can realize the monitoring imaging of high-resolution and fast calculation of microbubbles in the process of tissue damage, providing an important experimental basis for the clinical application of non-invasive transcranial ultrasound.
format	article
author	Li Yan Chen Bai Yu Zheng Xiaodong Zhou Mingxi Wan Yujin Zong Shanshan Chen Yin Zhou
author_facet	Li Yan Chen Bai Yu Zheng Xiaodong Zhou Mingxi Wan Yujin Zong Shanshan Chen Yin Zhou
author_sort	Li Yan
title	Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
title_short	Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
title_full	Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
title_fullStr	Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
title_full_unstemmed	Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys
title_sort	study on the application of super-resolution ultrasound for cerebral vessel imaging in rhesus monkeys
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2c9d9f13430d49309bac277ca40aa41b
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Study on the Application of Super-Resolution Ultrasound for Cerebral Vessel Imaging in Rhesus Monkeys

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