Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining

Abstract Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectros...

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Autores principales:	Shirly Berezin, Yaron Aviv, Hagit Aviv, Elad Goldberg, Yaakov R. Tischler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ca86e57c2da4172877049b806a9f39d
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spelling	oai:doaj.org-article:2ca86e57c2da4172877049b806a9f39d2021-12-02T12:32:07ZReplacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining10.1038/s41598-017-02212-22045-2322https://doaj.org/article/2ca86e57c2da4172877049b806a9f39d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02212-2https://doaj.org/toc/2045-2322Abstract Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria’s lipid-bilayer membrane, which specifically enhances Gram negative bacteria.Shirly BerezinYaron AvivHagit AvivElad GoldbergYaakov R. TischlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shirly Berezin Yaron Aviv Hagit Aviv Elad Goldberg Yaakov R. Tischler Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
description	Abstract Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria’s lipid-bilayer membrane, which specifically enhances Gram negative bacteria.
format	article
author	Shirly Berezin Yaron Aviv Hagit Aviv Elad Goldberg Yaakov R. Tischler
author_facet	Shirly Berezin Yaron Aviv Hagit Aviv Elad Goldberg Yaakov R. Tischler
author_sort	Shirly Berezin
title	Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_short	Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_full	Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_fullStr	Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_full_unstemmed	Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining
title_sort	replacing a century old technique – modern spectroscopy can supplant gram staining
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2ca86e57c2da4172877049b806a9f39d
work_keys_str_mv	AT shirlyberezin replacingacenturyoldtechniquemodernspectroscopycansupplantgramstaining AT yaronaviv replacingacenturyoldtechniquemodernspectroscopycansupplantgramstaining AT hagitaviv replacingacenturyoldtechniquemodernspectroscopycansupplantgramstaining AT eladgoldberg replacingacenturyoldtechniquemodernspectroscopycansupplantgramstaining AT yaakovrtischler replacingacenturyoldtechniquemodernspectroscopycansupplantgramstaining
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Replacing a Century Old Technique – Modern Spectroscopy Can Supplant Gram Staining

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