A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.

Magnetotactic bacteria are able to swim navigating along geomagnetic field lines. They synthesize ferromagnetic nanocrystals that are embedded in cytoplasmic membrane invaginations forming magnetosomes. Regularly aligned in the cytoplasm along cytoskeleton filaments, the magnetosome chain effectivel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jean-Baptiste Rioux, Nadège Philippe, Sandrine Pereira, David Pignol, Long-Fei Wu, Nicolas Ginet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cad0a2aebe144778fb09dca3b28c891
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2cad0a2aebe144778fb09dca3b28c891
record_format	dspace
spelling	oai:doaj.org-article:2cad0a2aebe144778fb09dca3b28c8912021-11-25T06:25:57ZA second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.1932-620310.1371/journal.pone.0009151https://doaj.org/article/2cad0a2aebe144778fb09dca3b28c8912010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20161777/?tool=EBIhttps://doaj.org/toc/1932-6203Magnetotactic bacteria are able to swim navigating along geomagnetic field lines. They synthesize ferromagnetic nanocrystals that are embedded in cytoplasmic membrane invaginations forming magnetosomes. Regularly aligned in the cytoplasm along cytoskeleton filaments, the magnetosome chain effectively forms a compass needle bestowing on bacteria their magnetotactic behaviour. A large genomic island, conserved among magnetotactic bacteria, contains the genes potentially involved in magnetosome formation. One of the genes, mamK has been described as encoding a prokaryotic actin-like protein which when it polymerizes forms in the cytoplasm filamentous structures that provide the scaffold for magnetosome alignment. Here, we have identified a series of genes highly similar to the mam genes in the genome of Magnetospirillum magneticum AMB-1. The newly annotated genes are clustered in a genomic islet distinct and distant from the known magnetosome genomic island and most probably acquired by lateral gene transfer rather than duplication. We focused on a mamK-like gene whose product shares 54.5% identity with the actin-like MamK. Filament bundles of polymerized MamK-like protein were observed in vitro with electron microscopy and in vivo in E. coli cells expressing MamK-like-Venus fusions by fluorescence microscopy. In addition, we demonstrate that mamK-like is transcribed in AMB-1 wild-type and DeltamamK mutant cells and that the actin-like filamentous structures observed in the DeltamamK strain are probably MamK-like polymers. Thus MamK-like is a new member of the prokaryotic actin-like family. This is the first evidence of a functional mam gene encoded outside the magnetosome genomic island.Jean-Baptiste RiouxNadège PhilippeSandrine PereiraDavid PignolLong-Fei WuNicolas GinetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9151 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jean-Baptiste Rioux Nadège Philippe Sandrine Pereira David Pignol Long-Fei Wu Nicolas Ginet A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
description	Magnetotactic bacteria are able to swim navigating along geomagnetic field lines. They synthesize ferromagnetic nanocrystals that are embedded in cytoplasmic membrane invaginations forming magnetosomes. Regularly aligned in the cytoplasm along cytoskeleton filaments, the magnetosome chain effectively forms a compass needle bestowing on bacteria their magnetotactic behaviour. A large genomic island, conserved among magnetotactic bacteria, contains the genes potentially involved in magnetosome formation. One of the genes, mamK has been described as encoding a prokaryotic actin-like protein which when it polymerizes forms in the cytoplasm filamentous structures that provide the scaffold for magnetosome alignment. Here, we have identified a series of genes highly similar to the mam genes in the genome of Magnetospirillum magneticum AMB-1. The newly annotated genes are clustered in a genomic islet distinct and distant from the known magnetosome genomic island and most probably acquired by lateral gene transfer rather than duplication. We focused on a mamK-like gene whose product shares 54.5% identity with the actin-like MamK. Filament bundles of polymerized MamK-like protein were observed in vitro with electron microscopy and in vivo in E. coli cells expressing MamK-like-Venus fusions by fluorescence microscopy. In addition, we demonstrate that mamK-like is transcribed in AMB-1 wild-type and DeltamamK mutant cells and that the actin-like filamentous structures observed in the DeltamamK strain are probably MamK-like polymers. Thus MamK-like is a new member of the prokaryotic actin-like family. This is the first evidence of a functional mam gene encoded outside the magnetosome genomic island.
format	article
author	Jean-Baptiste Rioux Nadège Philippe Sandrine Pereira David Pignol Long-Fei Wu Nicolas Ginet
author_facet	Jean-Baptiste Rioux Nadège Philippe Sandrine Pereira David Pignol Long-Fei Wu Nicolas Ginet
author_sort	Jean-Baptiste Rioux
title	A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
title_short	A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
title_full	A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
title_fullStr	A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
title_full_unstemmed	A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.
title_sort	second actin-like mamk protein in magnetospirillum magneticum amb-1 encoded outside the genomic magnetosome island.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/2cad0a2aebe144778fb09dca3b28c891
work_keys_str_mv	AT jeanbaptisterioux asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT nadegephilippe asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT sandrinepereira asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT davidpignol asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT longfeiwu asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT nicolasginet asecondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT jeanbaptisterioux secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT nadegephilippe secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT sandrinepereira secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT davidpignol secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT longfeiwu secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland AT nicolasginet secondactinlikemamkproteininmagnetospirillummagneticumamb1encodedoutsidethegenomicmagnetosomeisland
_version_	1718413731362242560

A second actin-like MamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island.

Ejemplares similares