Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons

Abstract Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We rep...

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Autores principales:	Florian Ströhl, Julie Qiaojin Lin, Romain F. Laine, Hovy Ho-Wai Wong, Vasja Urbančič, Roberta Cagnetta, Christine E. Holt, Clemens F. Kaminski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cb1a78828434275beac474015e8c8e8
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spelling	oai:doaj.org-article:2cb1a78828434275beac474015e8c8e82021-12-02T12:30:11ZSingle Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons10.1038/s41598-017-00695-72045-2322https://doaj.org/article/2cb1a78828434275beac474015e8c8e82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00695-7https://doaj.org/toc/2045-2322Abstract Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.Florian StröhlJulie Qiaojin LinRomain F. LaineHovy Ho-Wai WongVasja UrbančičRoberta CagnettaChristine E. HoltClemens F. KaminskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florian Ströhl Julie Qiaojin Lin Romain F. Laine Hovy Ho-Wai Wong Vasja Urbančič Roberta Cagnetta Christine E. Holt Clemens F. Kaminski Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
description	Abstract Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.
format	article
author	Florian Ströhl Julie Qiaojin Lin Romain F. Laine Hovy Ho-Wai Wong Vasja Urbančič Roberta Cagnetta Christine E. Holt Clemens F. Kaminski
author_facet	Florian Ströhl Julie Qiaojin Lin Romain F. Laine Hovy Ho-Wai Wong Vasja Urbančič Roberta Cagnetta Christine E. Holt Clemens F. Kaminski
author_sort	Florian Ströhl
title	Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_short	Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_full	Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_fullStr	Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_full_unstemmed	Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_sort	single molecule translation imaging visualizes the dynamics of local β-actin synthesis in retinal axons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2cb1a78828434275beac474015e8c8e8
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Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons

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