Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor

Abstract Peptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a confor...

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Autores principales:	Jessica Attia, Sivan Nir, Evgeniy Mervinetsky, Dora Balogh, Agata Gitlin-Domagalska, Israel Alshanski, Meital Reches, Mattan Hurevich, Shlomo Yitzchaik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cb250a498cc43428ff440e2178f5a6a
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spelling	oai:doaj.org-article:2cb250a498cc43428ff440e2178f5a6a2021-12-02T14:23:32ZNon-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor10.1038/s41598-021-85015-w2045-2322https://doaj.org/article/2cb250a498cc43428ff440e2178f5a6a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85015-whttps://doaj.org/toc/2045-2322Abstract Peptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a conformational change of the oxytocin peptide in the monolayer, which was measured using electrochemical impedance spectroscopy. Here, we demonstrate that sensing can be achieved also when the oxytocin is non-covalently integrated into an alkanethiol host monolayer. We show that ion-binding cause morphological changes to the dense host layer, which translates into enhanced impedimetric signals compared to direct covalent assembly strategies. This biosensor proved selective and sensitive for Zn2+ ions in the range of nano- to micro-molar concentrations. This strategy offers an approach to utilize peptide flexibility in monitoring their response to the environment while embedded in a hydrophobic monolayer.Jessica AttiaSivan NirEvgeniy MervinetskyDora BaloghAgata Gitlin-DomagalskaIsrael AlshanskiMeital RechesMattan HurevichShlomo YitzchaikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessica Attia Sivan Nir Evgeniy Mervinetsky Dora Balogh Agata Gitlin-Domagalska Israel Alshanski Meital Reches Mattan Hurevich Shlomo Yitzchaik Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
description	Abstract Peptides are commonly used as biosensors for analytes such as metal ions as they have natural binding preferences. In our previous peptide-based impedimetric metal ion biosensors, a monolayer of the peptide was anchored covalently to the electrode. Binding of metal ions resulted in a conformational change of the oxytocin peptide in the monolayer, which was measured using electrochemical impedance spectroscopy. Here, we demonstrate that sensing can be achieved also when the oxytocin is non-covalently integrated into an alkanethiol host monolayer. We show that ion-binding cause morphological changes to the dense host layer, which translates into enhanced impedimetric signals compared to direct covalent assembly strategies. This biosensor proved selective and sensitive for Zn2+ ions in the range of nano- to micro-molar concentrations. This strategy offers an approach to utilize peptide flexibility in monitoring their response to the environment while embedded in a hydrophobic monolayer.
format	article
author	Jessica Attia Sivan Nir Evgeniy Mervinetsky Dora Balogh Agata Gitlin-Domagalska Israel Alshanski Meital Reches Mattan Hurevich Shlomo Yitzchaik
author_facet	Jessica Attia Sivan Nir Evgeniy Mervinetsky Dora Balogh Agata Gitlin-Domagalska Israel Alshanski Meital Reches Mattan Hurevich Shlomo Yitzchaik
author_sort	Jessica Attia
title	Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
title_short	Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
title_full	Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
title_fullStr	Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
title_full_unstemmed	Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor
title_sort	non-covalently embedded oxytocin in alkanethiol monolayer as zn2+ selective biosensor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2cb250a498cc43428ff440e2178f5a6a
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Non-covalently embedded oxytocin in alkanethiol monolayer as Zn2+ selective biosensor

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