Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.

<h4>Objective</h4>This in vitro study characterized and monitored, by Optical Coherence Tomography (OCT) and Fourier Transformed Infrared Spectroscopy (FTIR), the effects of the association of acidulated phosphate fluoride gel (APF-gel) and Nd:YAG (neodymiun:yttrium-aluminum-garnet) lase...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marcia Cristina Dias-Moraes, Pedro Arthur Augusto Castro, Daísa Lima Pereira, Patrícia Aparecida Ana, Anderson Zanardi Freitas, Denise Maria Zezell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cb6fa9757bd472e8fc01d364d64315e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2cb6fa9757bd472e8fc01d364d64315e
record_format	dspace
spelling	oai:doaj.org-article:2cb6fa9757bd472e8fc01d364d64315e2021-12-02T20:15:36ZAssessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.1932-620310.1371/journal.pone.0254217https://doaj.org/article/2cb6fa9757bd472e8fc01d364d64315e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254217https://doaj.org/toc/1932-6203<h4>Objective</h4>This in vitro study characterized and monitored, by Optical Coherence Tomography (OCT) and Fourier Transformed Infrared Spectroscopy (FTIR), the effects of the association of acidulated phosphate fluoride gel (APF-gel) and Nd:YAG (neodymiun:yttrium-aluminum-garnet) laser, as sequencial treatments, in the prevention of incipient enamel caries lesions.<h4>Methods</h4>120 human enamel samples were randomized into 3 groups (n = 40): APF-gel (1.23% F-, 4 min.); Laser+APF (Nd:YAG laser irradiation-0.6W, 84.9J/cm2, 10Hz, followed by APF-gel); and APF+Laser (APF-gel followed by laser irradiation). The samples were subjected to a 15-day pH-cycling, evaluated by OCT (quantification of optical attenuation coefficient-OAC) and FTIR (analysis of carbonate and phosphate content) before treatments, after treatments, and on the 5th, 10th and 15th days of pH-cycling. The statistical analysis was performed (α = 5%).<h4>Results</h4>The Optical Attenuation Coefficient (OAC) assessed by OCT increases with the progression of demineralization, and the Laser+APF presented the highest values of OAC in 10th and 15th days of pH-cycling. Nd:YAG decreased the carbonate content after treatment regardless of the application order of the APF-gel, while APF-gel did not interfere in the composition of enamel. The carbonate content was also changed in the first 5 days of the pH-cycling in all groups.<h4>Conclusion</h4>Nd:YAG laser irradiation before or after the application of APF-gel did not influence the appearance of incipient caries lesions, showing no synergistic effect. Regardless of the application order of the APF-gel, laser irradiation reduces the carbonate content of the enamel, which also changes during the demineralization process. However, irradiation before the application of APF-gel increased the speed of progression of the lesions, which positively impacts public health as it can prevent caries disease, even in high risk individuals. OCT and FTIR are suitable for assessing this effect.Marcia Cristina Dias-MoraesPedro Arthur Augusto CastroDaísa Lima PereiraPatrícia Aparecida AnaAnderson Zanardi FreitasDenise Maria ZezellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254217 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcia Cristina Dias-Moraes Pedro Arthur Augusto Castro Daísa Lima Pereira Patrícia Aparecida Ana Anderson Zanardi Freitas Denise Maria Zezell Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
description	<h4>Objective</h4>This in vitro study characterized and monitored, by Optical Coherence Tomography (OCT) and Fourier Transformed Infrared Spectroscopy (FTIR), the effects of the association of acidulated phosphate fluoride gel (APF-gel) and Nd:YAG (neodymiun:yttrium-aluminum-garnet) laser, as sequencial treatments, in the prevention of incipient enamel caries lesions.<h4>Methods</h4>120 human enamel samples were randomized into 3 groups (n = 40): APF-gel (1.23% F-, 4 min.); Laser+APF (Nd:YAG laser irradiation-0.6W, 84.9J/cm2, 10Hz, followed by APF-gel); and APF+Laser (APF-gel followed by laser irradiation). The samples were subjected to a 15-day pH-cycling, evaluated by OCT (quantification of optical attenuation coefficient-OAC) and FTIR (analysis of carbonate and phosphate content) before treatments, after treatments, and on the 5th, 10th and 15th days of pH-cycling. The statistical analysis was performed (α = 5%).<h4>Results</h4>The Optical Attenuation Coefficient (OAC) assessed by OCT increases with the progression of demineralization, and the Laser+APF presented the highest values of OAC in 10th and 15th days of pH-cycling. Nd:YAG decreased the carbonate content after treatment regardless of the application order of the APF-gel, while APF-gel did not interfere in the composition of enamel. The carbonate content was also changed in the first 5 days of the pH-cycling in all groups.<h4>Conclusion</h4>Nd:YAG laser irradiation before or after the application of APF-gel did not influence the appearance of incipient caries lesions, showing no synergistic effect. Regardless of the application order of the APF-gel, laser irradiation reduces the carbonate content of the enamel, which also changes during the demineralization process. However, irradiation before the application of APF-gel increased the speed of progression of the lesions, which positively impacts public health as it can prevent caries disease, even in high risk individuals. OCT and FTIR are suitable for assessing this effect.
format	article
author	Marcia Cristina Dias-Moraes Pedro Arthur Augusto Castro Daísa Lima Pereira Patrícia Aparecida Ana Anderson Zanardi Freitas Denise Maria Zezell
author_facet	Marcia Cristina Dias-Moraes Pedro Arthur Augusto Castro Daísa Lima Pereira Patrícia Aparecida Ana Anderson Zanardi Freitas Denise Maria Zezell
author_sort	Marcia Cristina Dias-Moraes
title	Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
title_short	Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
title_full	Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
title_fullStr	Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
title_full_unstemmed	Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.
title_sort	assessment of the preventive effects of nd:yag laser associated with fluoride on enamel caries using optical coherence tomography and ftir spectroscopy.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2cb6fa9757bd472e8fc01d364d64315e
work_keys_str_mv	AT marciacristinadiasmoraes assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy AT pedroarthuraugustocastro assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy AT daisalimapereira assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy AT patriciaaparecidaana assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy AT andersonzanardifreitas assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy AT denisemariazezell assessmentofthepreventiveeffectsofndyaglaserassociatedwithfluorideonenamelcariesusingopticalcoherencetomographyandftirspectroscopy
_version_	1718374514951192576

Assessment of the preventive effects of Nd:YAG laser associated with fluoride on enamel caries using optical coherence tomography and FTIR spectroscopy.

Ejemplares similares