Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

Satya Ranjan Sarker, Yumiko Aoshima, Ryosuke Hokama, Takafumi Inoue, Keitaro Sou, Shinji Takeoka Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), Tokyo, Japan Background: Currently available gene delivery vehicles have...

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Autores principales:	Sarker SR, Aoshima Y, Hokama R, Inoue T, Sou K, Takeoka S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2cbe2d34aa8c45969cf0ff06fbdac55d
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spelling	oai:doaj.org-article:2cbe2d34aa8c45969cf0ff06fbdac55d2021-12-02T02:04:22ZArginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity1176-91141178-2013https://doaj.org/article/2cbe2d34aa8c45969cf0ff06fbdac55d2013-04-01T00:00:00Zhttp://www.dovepress.com/arginine-based-cationic-liposomes-for-efficient-in-vitro-plasmid-dna-d-a12709https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Satya Ranjan Sarker, Yumiko Aoshima, Ryosuke Hokama, Takafumi Inoue, Keitaro Sou, Shinji Takeoka Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), Tokyo, Japan Background: Currently available gene delivery vehicles have many limitations such as low gene delivery efficiency and high cytotoxicity. To overcome these drawbacks, we designed and synthesized two cationic lipids comprised of n-tetradecyl alcohol as the hydrophobic moiety, 3-hydrocarbon chain as the spacer, and different counterions (eg, hydrogen chloride [HCl] salt or trifluoroacetic acid [TFA] salt) in the arginine head group. Methods: Cationic lipids were hydrated in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer to prepare cationic liposomes and characterized in terms of their size, zeta potential, phase transition temperature, and morphology. Lipoplexes were then prepared and characterized in terms of their size and zeta potential in the absence or presence of serum. The morphology of the lipoplexes was determined using transmission electron microscopy and atomic force microscopy. The gene delivery efficiency was evaluated in neuronal cells and HeLa cells and compared with that of lysine-based cationic assemblies and Lipofectamine™ 2000. The cytotoxicity level of the cationic lipids was investigated and compared with that of Lipofectamine™ 2000. Results: We synthesized arginine-based cationic lipids having different counterions (ie, HCl-salt or TFA-salt) that formed cationic liposomes of around 100 nm in size. In the absence of serum, lipoplexes prepared from the arginine-based cationic liposomes and plasmid (p) DNA formed large aggregates and attained a positive zeta potential. However, in the presence of serum, the lipoplexes were smaller in size and negative in zeta potential. The morphology of the lipoplexes was vesicular. Arginine-based cationic liposomes with HCl-salt showed the highest transfection efficiency in PC-12 cells. However, arginine-based cationic liposomes with TFA salt showed the highest transfection efficiency in HeLa cells, regardless of the presence of serum, with very low associated cytotoxicity. Conclusion: The gene delivery efficiency of amino acid-based cationic assemblies is influenced by the amino acids (ie, arginine or lysine) present as the hydrophilic head group and their associated counterions. Keywords: cationic liposome, transfection efficiency, cytotoxicity, counterions, pDNASarker SRAoshima YHokama RInoue TSou KTakeoka SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1361-1375 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sarker SR Aoshima Y Hokama R Inoue T Sou K Takeoka S Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
description	Satya Ranjan Sarker, Yumiko Aoshima, Ryosuke Hokama, Takafumi Inoue, Keitaro Sou, Shinji Takeoka Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University (TWIns), Tokyo, Japan Background: Currently available gene delivery vehicles have many limitations such as low gene delivery efficiency and high cytotoxicity. To overcome these drawbacks, we designed and synthesized two cationic lipids comprised of n-tetradecyl alcohol as the hydrophobic moiety, 3-hydrocarbon chain as the spacer, and different counterions (eg, hydrogen chloride [HCl] salt or trifluoroacetic acid [TFA] salt) in the arginine head group. Methods: Cationic lipids were hydrated in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer to prepare cationic liposomes and characterized in terms of their size, zeta potential, phase transition temperature, and morphology. Lipoplexes were then prepared and characterized in terms of their size and zeta potential in the absence or presence of serum. The morphology of the lipoplexes was determined using transmission electron microscopy and atomic force microscopy. The gene delivery efficiency was evaluated in neuronal cells and HeLa cells and compared with that of lysine-based cationic assemblies and Lipofectamine™ 2000. The cytotoxicity level of the cationic lipids was investigated and compared with that of Lipofectamine™ 2000. Results: We synthesized arginine-based cationic lipids having different counterions (ie, HCl-salt or TFA-salt) that formed cationic liposomes of around 100 nm in size. In the absence of serum, lipoplexes prepared from the arginine-based cationic liposomes and plasmid (p) DNA formed large aggregates and attained a positive zeta potential. However, in the presence of serum, the lipoplexes were smaller in size and negative in zeta potential. The morphology of the lipoplexes was vesicular. Arginine-based cationic liposomes with HCl-salt showed the highest transfection efficiency in PC-12 cells. However, arginine-based cationic liposomes with TFA salt showed the highest transfection efficiency in HeLa cells, regardless of the presence of serum, with very low associated cytotoxicity. Conclusion: The gene delivery efficiency of amino acid-based cationic assemblies is influenced by the amino acids (ie, arginine or lysine) present as the hydrophilic head group and their associated counterions. Keywords: cationic liposome, transfection efficiency, cytotoxicity, counterions, pDNA
format	article
author	Sarker SR Aoshima Y Hokama R Inoue T Sou K Takeoka S
author_facet	Sarker SR Aoshima Y Hokama R Inoue T Sou K Takeoka S
author_sort	Sarker SR
title	Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
title_short	Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
title_full	Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
title_fullStr	Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
title_full_unstemmed	Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
title_sort	arginine-based cationic liposomes for efficient in vitro plasmid dna delivery with low cytotoxicity
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/2cbe2d34aa8c45969cf0ff06fbdac55d
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Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity

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