Conversion of Lignocellulosic Material Into Fermentable Sugars

Enzymatic hydrolysis process of lignocellulosic biomass materials is difficult because of inherent structural features of biomass, which represents barriers that prevent complete hydrolysis; therefore, pretreatment techniques are necessary to render biomass highly digestible in enzymatic hydrolysis...

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Autores principales: Asem Hassan Mohammed, Frank Behrendt Behrendt, Frank Jürgen Methner
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Publicado: Al-Khwarizmi College of Engineering – University of Baghdad 2017
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Acceso en línea:https://doaj.org/article/2cbf77a7c56042f0915ef65ed1ccb80c
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spelling oai:doaj.org-article:2cbf77a7c56042f0915ef65ed1ccb80c2021-12-02T07:16:39ZConversion of Lignocellulosic Material Into Fermentable Sugars10.22153/kej.2016.05.0061818-11712312-0789https://doaj.org/article/2cbf77a7c56042f0915ef65ed1ccb80c2017-12-01T00:00:00Zhttp://alkej.uobaghdad.edu.iq/index.php/alkej/article/view/329https://doaj.org/toc/1818-1171https://doaj.org/toc/2312-0789 Enzymatic hydrolysis process of lignocellulosic biomass materials is difficult because of inherent structural features of biomass, which represents barriers that prevent complete hydrolysis; therefore, pretreatment techniques are necessary to render biomass highly digestible in enzymatic hydrolysis process. In this research, (non?) oxidative short-term lime pretreatment of willow wood was used. A weight of  11.40 g of willow wood was mixed with an excess of calcium hydroxide (0.4 g Ca(OH)2/g raw biomass) and water loading (15 g/g raw biomass). Lime pretreatment was carried out for various periods of time including 1, 2, 3.5, 5 and 6 h, with temperatures at 100, 113, 130, 147 and 1600C, and oxygen pressures as oxidativeagent (6, 9, 13.5, 17.8, 21 bar absolute). The optimization of both pretreatment and enzymatic hydrolysis were depended on the maximum overall yields of glucan and xylan after two processes of lime pretreatment and enzymatic hydrolysis. The optimal conditions of pretreatment were as follow: 1) 1.33 h, 1470C, 17.8 bar absolute, 0.26 g Ca(OH)2/g raw biomass. 2) 1.25 h, 155 0C, 21 bar absolute, 0.26 Ca(OH)2/g raw biomass. Furthermore, the optimal values for low impact factors such as water loading was 15 g/g raw biomass and particle size was less than 3 mm. The optimal conditions of enzymatic hydrolysis were as follow: Cellulase enzymeloading was 0.1 g /g glucan in raw biomass, at substrate concentration of 50 g/L during 72 h of enzymatic hydrolysis The yield of enzymatic hydrolysis under these conditions were as follow: 96.00 g glucan/100 g of glucan in raw biomass, and 65.00 g xylan/100 g xylan in raw biomass. Asem Hassan MohammedFrank Behrendt BehrendtFrank Jürgen MethnerAl-Khwarizmi College of Engineering – University of Baghdadarticlelime pretreatmentenzymatic hydrolysislignocellulosic biomasswillow woodChemical engineeringTP155-156Engineering (General). Civil engineering (General)TA1-2040ENAl-Khawarizmi Engineering Journal, Vol 12, Iss 4 (2017)
institution DOAJ
collection DOAJ
language EN
topic lime pretreatment
enzymatic hydrolysis
lignocellulosic biomass
willow wood
Chemical engineering
TP155-156
Engineering (General). Civil engineering (General)
TA1-2040
spellingShingle lime pretreatment
enzymatic hydrolysis
lignocellulosic biomass
willow wood
Chemical engineering
TP155-156
Engineering (General). Civil engineering (General)
TA1-2040
Asem Hassan Mohammed
Frank Behrendt Behrendt
Frank Jürgen Methner
Conversion of Lignocellulosic Material Into Fermentable Sugars
description Enzymatic hydrolysis process of lignocellulosic biomass materials is difficult because of inherent structural features of biomass, which represents barriers that prevent complete hydrolysis; therefore, pretreatment techniques are necessary to render biomass highly digestible in enzymatic hydrolysis process. In this research, (non?) oxidative short-term lime pretreatment of willow wood was used. A weight of  11.40 g of willow wood was mixed with an excess of calcium hydroxide (0.4 g Ca(OH)2/g raw biomass) and water loading (15 g/g raw biomass). Lime pretreatment was carried out for various periods of time including 1, 2, 3.5, 5 and 6 h, with temperatures at 100, 113, 130, 147 and 1600C, and oxygen pressures as oxidativeagent (6, 9, 13.5, 17.8, 21 bar absolute). The optimization of both pretreatment and enzymatic hydrolysis were depended on the maximum overall yields of glucan and xylan after two processes of lime pretreatment and enzymatic hydrolysis. The optimal conditions of pretreatment were as follow: 1) 1.33 h, 1470C, 17.8 bar absolute, 0.26 g Ca(OH)2/g raw biomass. 2) 1.25 h, 155 0C, 21 bar absolute, 0.26 Ca(OH)2/g raw biomass. Furthermore, the optimal values for low impact factors such as water loading was 15 g/g raw biomass and particle size was less than 3 mm. The optimal conditions of enzymatic hydrolysis were as follow: Cellulase enzymeloading was 0.1 g /g glucan in raw biomass, at substrate concentration of 50 g/L during 72 h of enzymatic hydrolysis The yield of enzymatic hydrolysis under these conditions were as follow: 96.00 g glucan/100 g of glucan in raw biomass, and 65.00 g xylan/100 g xylan in raw biomass.
format article
author Asem Hassan Mohammed
Frank Behrendt Behrendt
Frank Jürgen Methner
author_facet Asem Hassan Mohammed
Frank Behrendt Behrendt
Frank Jürgen Methner
author_sort Asem Hassan Mohammed
title Conversion of Lignocellulosic Material Into Fermentable Sugars
title_short Conversion of Lignocellulosic Material Into Fermentable Sugars
title_full Conversion of Lignocellulosic Material Into Fermentable Sugars
title_fullStr Conversion of Lignocellulosic Material Into Fermentable Sugars
title_full_unstemmed Conversion of Lignocellulosic Material Into Fermentable Sugars
title_sort conversion of lignocellulosic material into fermentable sugars
publisher Al-Khwarizmi College of Engineering – University of Baghdad
publishDate 2017
url https://doaj.org/article/2cbf77a7c56042f0915ef65ed1ccb80c
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