Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment

Yung-Chih Kuo,1 Che-Yu Lin,1 Jay-Shake Li,2 Yung-I Lou3 1Department of Chemical Engineering, 2Department of Psychology, National Chung Cheng University, Chia-Yi, 3Department of Accounting, Providence University, Taichung, Taiwan, Republic of China Abstract: Curcumin (CRM) and nerve growth factor (...

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Autores principales: Kuo YC, Lin CY, Li JS, Lou YI
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
liposome
Alzheimer's disease
β-amyloid
neurodegeneration
blood–brain barrier
wheat germ agglutinin
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/2cbfcf542a2049e6ba4fb5364f5dbb94
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spelling oai:doaj.org-article:2cbfcf542a2049e6ba4fb5364f5dbb942021-12-02T00:09:56ZWheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment1178-2013https://doaj.org/article/2cbfcf542a2049e6ba4fb5364f5dbb942017-03-01T00:00:00Zhttps://www.dovepress.com/wheat-germ-agglutinin-conjugated-liposomes-incorporated-with-cardiolip-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yung-Chih Kuo,1 Che-Yu Lin,1 Jay-Shake Li,2 Yung-I Lou3 1Department of Chemical Engineering, 2Department of Psychology, National Chung Cheng University, Chia-Yi, 3Department of Accounting, Providence University, Taichung, Taiwan, Republic of China Abstract: Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer’s disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells. In addition, NGF-CL/LIP could enhance the quantities of p-neurotrophic tyrosine kinase receptor type 1 and p-extracellular signal-regulated kinase 5 for neuronal rescue. Moreover, WGA-grafted CRM-CL/LIP and WGA-grafted NGF-CL/LIP significantly improved the permeation of CRM and NGF across the blood–brain barrier, reduced Aβ plaque deposition and the malondialdehyde level, and increased the percentage of normal neurons and cholinergic activity in the hippocampus of AD rats. Based on the marker expressions and in vivo evidence, current LIP carriers can be promising drug delivery systems to protect nervous tissue against Aβ-induced apoptosis in the brain during the clinical management of AD. Keywords: liposome, Alzheimer’s disease, β-amyloid, neurodegeneration, blood–brain barrier, wheat germ agglutininKuo YCLin CYLi JSLou YIDove Medical PressarticleliposomeAlzheimer's diseaseβ-amyloidneurodegenerationblood–brain barrierwheat germ agglutininMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1757-1774 (2017)
institution DOAJ
collection DOAJ
language EN
topic liposome
Alzheimer's disease
β-amyloid
neurodegeneration
blood–brain barrier
wheat germ agglutinin
Medicine (General)
R5-920
spellingShingle liposome
Alzheimer's disease
β-amyloid
neurodegeneration
blood–brain barrier
wheat germ agglutinin
Medicine (General)
R5-920
Kuo YC
Lin CY
Li JS
Lou YI
Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
description Yung-Chih Kuo,1 Che-Yu Lin,1 Jay-Shake Li,2 Yung-I Lou3 1Department of Chemical Engineering, 2Department of Psychology, National Chung Cheng University, Chia-Yi, 3Department of Accounting, Providence University, Taichung, Taiwan, Republic of China Abstract: Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer’s disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells. In addition, NGF-CL/LIP could enhance the quantities of p-neurotrophic tyrosine kinase receptor type 1 and p-extracellular signal-regulated kinase 5 for neuronal rescue. Moreover, WGA-grafted CRM-CL/LIP and WGA-grafted NGF-CL/LIP significantly improved the permeation of CRM and NGF across the blood–brain barrier, reduced Aβ plaque deposition and the malondialdehyde level, and increased the percentage of normal neurons and cholinergic activity in the hippocampus of AD rats. Based on the marker expressions and in vivo evidence, current LIP carriers can be promising drug delivery systems to protect nervous tissue against Aβ-induced apoptosis in the brain during the clinical management of AD. Keywords: liposome, Alzheimer’s disease, β-amyloid, neurodegeneration, blood–brain barrier, wheat germ agglutinin
format article
author Kuo YC
Lin CY
Li JS
Lou YI
author_facet Kuo YC
Lin CY
Li JS
Lou YI
author_sort Kuo YC
title Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
title_short Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
title_full Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
title_fullStr Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
title_full_unstemmed Wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in Alzheimer’s disease treatment
title_sort wheat germ agglutinin-conjugated liposomes incorporated with cardiolipin to improve neuronal survival in alzheimer’s disease treatment
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/2cbfcf542a2049e6ba4fb5364f5dbb94
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AT lincy wheatgermagglutininconjugatedliposomesincorporatedwithcardiolipintoimproveneuronalsurvivalinalzheimerrsquosdiseasetreatment
AT lijs wheatgermagglutininconjugatedliposomesincorporatedwithcardiolipintoimproveneuronalsurvivalinalzheimerrsquosdiseasetreatment
AT louyi wheatgermagglutininconjugatedliposomesincorporatedwithcardiolipintoimproveneuronalsurvivalinalzheimerrsquosdiseasetreatment
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