Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.

Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as we...

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Autores principales:	Sherin Kaul, L Sai Keerthana, Pankaj Kumar, Komal Birader, Yathirajarao Tammineni, Deepali Rawat, Pankaj Suman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/2cc4764a180a4925afb443f9887461b9
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spelling	oai:doaj.org-article:2cc4764a180a4925afb443f9887461b92021-11-25T06:33:24ZCytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.1935-27271935-273510.1371/journal.pntd.0009841https://doaj.org/article/2cc4764a180a4925afb443f9887461b92021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009841https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1 - κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of Naja naja (p < 0.0001) and Bungarus caeruleus (p<0.0001), without showing any immunoreactivity against the viperidae snakes in big four venomous snakes. The kit developed attained the limit of quantitation of 170 pg/μL and 2.1 ng/μL in spiked buffer samples and 28.7 ng/μL and 110 ng/μL in spiked serum samples for detection of N. naja and B. caeruleus venoms, respectively. This kit holds enormous potential in identification of elapid venom of the big four snakes for effective prognosis of an envenomation; as per the existing medical guidelines.Sherin KaulL Sai KeerthanaPankaj KumarKomal BiraderYathirajarao TammineniDeepali RawatPankaj SumanPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009841 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sherin Kaul L Sai Keerthana Pankaj Kumar Komal Birader Yathirajarao Tammineni Deepali Rawat Pankaj Suman Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
description	Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1 - κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of Naja naja (p < 0.0001) and Bungarus caeruleus (p<0.0001), without showing any immunoreactivity against the viperidae snakes in big four venomous snakes. The kit developed attained the limit of quantitation of 170 pg/μL and 2.1 ng/μL in spiked buffer samples and 28.7 ng/μL and 110 ng/μL in spiked serum samples for detection of N. naja and B. caeruleus venoms, respectively. This kit holds enormous potential in identification of elapid venom of the big four snakes for effective prognosis of an envenomation; as per the existing medical guidelines.
format	article
author	Sherin Kaul L Sai Keerthana Pankaj Kumar Komal Birader Yathirajarao Tammineni Deepali Rawat Pankaj Suman
author_facet	Sherin Kaul L Sai Keerthana Pankaj Kumar Komal Birader Yathirajarao Tammineni Deepali Rawat Pankaj Suman
author_sort	Sherin Kaul
title	Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
title_short	Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
title_full	Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
title_fullStr	Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
title_full_unstemmed	Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
title_sort	cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2cc4764a180a4925afb443f9887461b9
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Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.

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