Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex

The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, h...

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Autores principales:	Dmitrij Dedukh, Anatolie Marta, Karel Janko
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gynogenesis meiosis hybrid sterility endoreplication polyploidy <i>Cobitis taenia</i> complex Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2cc5211edafd4bb08e3278c2c5ddfe03
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spelling	oai:doaj.org-article:2cc5211edafd4bb08e3278c2c5ddfe032021-11-25T17:53:40ZChallenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex10.3390/ijms2222121171422-00671661-6596https://doaj.org/article/2cc5211edafd4bb08e3278c2c5ddfe032021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12117https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: <i>Cobitis</i>) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential.Dmitrij DedukhAnatolie MartaKarel JankoMDPI AGarticlegynogenesismeiosishybrid sterilityendoreplicationpolyploidy<i>Cobitis taenia</i> complexBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12117, p 12117 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gynogenesis meiosis hybrid sterility endoreplication polyploidy <i>Cobitis taenia</i> complex Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	gynogenesis meiosis hybrid sterility endoreplication polyploidy <i>Cobitis taenia</i> complex Biology (General) QH301-705.5 Chemistry QD1-999 Dmitrij Dedukh Anatolie Marta Karel Janko Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
description	The transition from sexual reproduction to asexuality is often triggered by hybridization. The gametogenesis of many hybrid asexuals involves premeiotic genome endoreplication leading to bypass hybrid sterility and forming clonal gametes. However, it is still not clear when endoreplication occurs, how many gonial cells it affects and whether its rate differs among clonal lineages. Here, we investigated meiotic and premeiotic cells of diploid and triploid hybrids of spined loaches (Cypriniformes: <i>Cobitis</i>) that reproduce by gynogenesis. We found that in naturally and experimentally produced F1 hybrids asexuality is achieved by genome endoreplication, which occurs in gonocytes just before entering meiosis or, rarely, one or a few divisions before meiosis. However, genome endoreplication was observed only in a minor fraction of the hybrid’s gonocytes, while the vast majority of gonocytes were unable to duplicate their genomes and consequently could not proceed beyond pachytene due to defects in bivalent formation. We also noted that the rate of endoreplication was significantly higher among gonocytes of hybrids from natural clones than of experimentally produced F1 hybrids. Thus, asexuality and hybrid sterility are intimately related phenomena and the transition from sexual reproduction to asexuality must overcome significant problems with genome incompatibilities with a possible impact on reproductive potential.
format	article
author	Dmitrij Dedukh Anatolie Marta Karel Janko
author_facet	Dmitrij Dedukh Anatolie Marta Karel Janko
author_sort	Dmitrij Dedukh
title	Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
title_short	Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
title_full	Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
title_fullStr	Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
title_full_unstemmed	Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex
title_sort	challenges and costs of asexuality: variation in premeiotic genome duplication in gynogenetic hybrids from <i>cobitis taenia</i> complex
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2cc5211edafd4bb08e3278c2c5ddfe03
work_keys_str_mv	AT dmitrijdedukh challengesandcostsofasexualityvariationinpremeioticgenomeduplicationingynogenetichybridsfromicobitistaeniaicomplex AT anatoliemarta challengesandcostsofasexualityvariationinpremeioticgenomeduplicationingynogenetichybridsfromicobitistaeniaicomplex AT kareljanko challengesandcostsofasexualityvariationinpremeioticgenomeduplicationingynogenetichybridsfromicobitistaeniaicomplex
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Challenges and Costs of Asexuality: Variation in Premeiotic Genome Duplication in Gynogenetic Hybrids from <i>Cobitis taenia</i> Complex

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