Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.

The flesh of the taproot of Raphanus sativus L. is rich in chlorophyll (Chl) throughout the developmental process, which is why the flesh is green. However, little is known about which genes are associated with Chl accumulation in this non-foliar, internal green tissue and whether the green flesh ca...

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Autores principales:	Yuan-Yuan Li, Min Han, Rui-Hua Wang, Ming-Gang Gao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cc9ef04c61e4040aa4a26757d01dd88
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spelling	oai:doaj.org-article:2cc9ef04c61e4040aa4a26757d01dd882021-11-25T06:23:44ZComparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.1932-620310.1371/journal.pone.0252031https://doaj.org/article/2cc9ef04c61e4040aa4a26757d01dd882021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252031https://doaj.org/toc/1932-6203The flesh of the taproot of Raphanus sativus L. is rich in chlorophyll (Chl) throughout the developmental process, which is why the flesh is green. However, little is known about which genes are associated with Chl accumulation in this non-foliar, internal green tissue and whether the green flesh can perform photosynthesis. To determine these aspects, we measured the Chl content, examined Chl fluorescence, and carried out comparative transcriptome analyses of taproot flesh between green-fleshed "Cuishuai" and white-fleshed "Zhedachang" across five developmental stages. Numerous genes involved in the Chl metabolic pathway were identified. It was found that Chl accumulation in radish green flesh may be due to the low expression of Chl degradation genes and high expression of Chl biosynthesis genes, especially those associated with Part Ⅳ (from Protoporphyrin Ⅸ to Chl a). Bioinformatics analysis revealed that differentially expressed genes between "Cuishuai" and "Zhedachang" were significantly enriched in photosynthesis-related pathways, such as photosynthesis, antenna proteins, porphyrin and Chl metabolism, carbon fixation, and photorespiration. Twenty-five genes involved in the Calvin cycle were highly expressed in "Cuishuai". These findings suggested that photosynthesis occurred in the radish green flesh, which was also supported by the results of Chl fluorescence. Our study provides transcriptome data on radish taproots and provides new information on the formation and function of radish green flesh.Yuan-Yuan LiMin HanRui-Hua WangMing-Gang GaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252031 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuan-Yuan Li Min Han Rui-Hua Wang Ming-Gang Gao Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
description	The flesh of the taproot of Raphanus sativus L. is rich in chlorophyll (Chl) throughout the developmental process, which is why the flesh is green. However, little is known about which genes are associated with Chl accumulation in this non-foliar, internal green tissue and whether the green flesh can perform photosynthesis. To determine these aspects, we measured the Chl content, examined Chl fluorescence, and carried out comparative transcriptome analyses of taproot flesh between green-fleshed "Cuishuai" and white-fleshed "Zhedachang" across five developmental stages. Numerous genes involved in the Chl metabolic pathway were identified. It was found that Chl accumulation in radish green flesh may be due to the low expression of Chl degradation genes and high expression of Chl biosynthesis genes, especially those associated with Part Ⅳ (from Protoporphyrin Ⅸ to Chl a). Bioinformatics analysis revealed that differentially expressed genes between "Cuishuai" and "Zhedachang" were significantly enriched in photosynthesis-related pathways, such as photosynthesis, antenna proteins, porphyrin and Chl metabolism, carbon fixation, and photorespiration. Twenty-five genes involved in the Calvin cycle were highly expressed in "Cuishuai". These findings suggested that photosynthesis occurred in the radish green flesh, which was also supported by the results of Chl fluorescence. Our study provides transcriptome data on radish taproots and provides new information on the formation and function of radish green flesh.
format	article
author	Yuan-Yuan Li Min Han Rui-Hua Wang Ming-Gang Gao
author_facet	Yuan-Yuan Li Min Han Rui-Hua Wang Ming-Gang Gao
author_sort	Yuan-Yuan Li
title	Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
title_short	Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
title_full	Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
title_fullStr	Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
title_full_unstemmed	Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
title_sort	comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2cc9ef04c61e4040aa4a26757d01dd88
work_keys_str_mv	AT yuanyuanli comparativetranscriptomeanalysisidentifiesgenesassociatedwithchlorophylllevelsandrevealsphotosynthesisingreenfleshofradishtaproot AT minhan comparativetranscriptomeanalysisidentifiesgenesassociatedwithchlorophylllevelsandrevealsphotosynthesisingreenfleshofradishtaproot AT ruihuawang comparativetranscriptomeanalysisidentifiesgenesassociatedwithchlorophylllevelsandrevealsphotosynthesisingreenfleshofradishtaproot AT mingganggao comparativetranscriptomeanalysisidentifiesgenesassociatedwithchlorophylllevelsandrevealsphotosynthesisingreenfleshofradishtaproot
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Comparative transcriptome analysis identifies genes associated with chlorophyll levels and reveals photosynthesis in green flesh of radish taproot.

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