Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation

Abstract A growing body of literature has recognized the non-thermal effect of pulsed microwave radiation (PMR) on bacterial systems. However, its mode of action in deactivating bacteria has not yet been extensively investigated. Nevertheless, it is highly important to advance the applications of PM...

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Autores principales:	Priyanka Shaw, Naresh Kumar, Sohail Mumtaz, Jun Sup Lim, Jung Hyun Jang, Doyoung Kim, Bidya Dhar Sahu, Annemie Bogaerts, Eun Ha Choi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cca9bba57f44b269f76998218cab791
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spelling	oai:doaj.org-article:2cca9bba57f44b269f76998218cab7912021-12-02T15:39:40ZEvaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation10.1038/s41598-021-93274-w2045-2322https://doaj.org/article/2cca9bba57f44b269f76998218cab7912021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93274-whttps://doaj.org/toc/2045-2322Abstract A growing body of literature has recognized the non-thermal effect of pulsed microwave radiation (PMR) on bacterial systems. However, its mode of action in deactivating bacteria has not yet been extensively investigated. Nevertheless, it is highly important to advance the applications of PMR from simple to complex biological systems. In this study, we first optimized the conditions of the PMR device and we assessed the results by simulations, using ANSYS HFSS (High Frequency Structure Simulator) and a 3D particle-in-cell code for the electron behavior, to provide a better overview of the bacterial cell exposure to microwave radiation. To determine the sensitivity of PMR, Escherichia coli and Staphylococcus aureus cultures were exposed to PMR (pulse duration: 60 ns, peak frequency: 3.5 GHz) with power density of 17 kW/cm2 at the free space of sample position, which would induce electric field of 8.0 kV/cm inside the PBS solution of falcon tube in this experiment at 25 °C. At various discharges (D) of microwaves, the colony forming unit curves were analyzed. The highest ratios of viable count reductions were observed when the doses were increased from 20D to 80D, which resulted in an approximate 6 log reduction in E. coli and 4 log reduction in S. aureus. Moreover, scanning electron microscopy also revealed surface damage in both bacterial strains after PMR exposure. The bacterial inactivation was attributed to the deactivation of oxidation-regulating genes and DNA damage.Priyanka ShawNaresh KumarSohail MumtazJun Sup LimJung Hyun JangDoyoung KimBidya Dhar SahuAnnemie BogaertsEun Ha ChoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Priyanka Shaw Naresh Kumar Sohail Mumtaz Jun Sup Lim Jung Hyun Jang Doyoung Kim Bidya Dhar Sahu Annemie Bogaerts Eun Ha Choi Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
description	Abstract A growing body of literature has recognized the non-thermal effect of pulsed microwave radiation (PMR) on bacterial systems. However, its mode of action in deactivating bacteria has not yet been extensively investigated. Nevertheless, it is highly important to advance the applications of PMR from simple to complex biological systems. In this study, we first optimized the conditions of the PMR device and we assessed the results by simulations, using ANSYS HFSS (High Frequency Structure Simulator) and a 3D particle-in-cell code for the electron behavior, to provide a better overview of the bacterial cell exposure to microwave radiation. To determine the sensitivity of PMR, Escherichia coli and Staphylococcus aureus cultures were exposed to PMR (pulse duration: 60 ns, peak frequency: 3.5 GHz) with power density of 17 kW/cm2 at the free space of sample position, which would induce electric field of 8.0 kV/cm inside the PBS solution of falcon tube in this experiment at 25 °C. At various discharges (D) of microwaves, the colony forming unit curves were analyzed. The highest ratios of viable count reductions were observed when the doses were increased from 20D to 80D, which resulted in an approximate 6 log reduction in E. coli and 4 log reduction in S. aureus. Moreover, scanning electron microscopy also revealed surface damage in both bacterial strains after PMR exposure. The bacterial inactivation was attributed to the deactivation of oxidation-regulating genes and DNA damage.
format	article
author	Priyanka Shaw Naresh Kumar Sohail Mumtaz Jun Sup Lim Jung Hyun Jang Doyoung Kim Bidya Dhar Sahu Annemie Bogaerts Eun Ha Choi
author_facet	Priyanka Shaw Naresh Kumar Sohail Mumtaz Jun Sup Lim Jung Hyun Jang Doyoung Kim Bidya Dhar Sahu Annemie Bogaerts Eun Ha Choi
author_sort	Priyanka Shaw
title	Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
title_short	Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
title_full	Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
title_fullStr	Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
title_full_unstemmed	Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
title_sort	evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2cca9bba57f44b269f76998218cab791
work_keys_str_mv	AT priyankashaw evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT nareshkumar evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT sohailmumtaz evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT junsuplim evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT junghyunjang evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT doyoungkim evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT bidyadharsahu evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT annemiebogaerts evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation AT eunhachoi evaluationofnonthermaleffectofmicrowaveradiationanditsmodeofactioninbacterialcellinactivation
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Evaluation of non-thermal effect of microwave radiation and its mode of action in bacterial cell inactivation

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