Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters

In order to increase the knowledge about geo-bio interactions in extreme metal-polluted mine waters, we combined microbiological, mineralogical, and geochemical analyses to study the indigenous sulfate-reducing bacteria (SRB) involved in the heavy metal (HM) biomineralization processes occurring in...

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Autores principales:	Patrizia Paganin, Chiara Alisi, Elisabetta Dore, Dario Fancello, Pier Andrea Marras, Daniela Medas, Maria Rita Montereali, Stefano Naitza, Nicola Rigonat, Anna Rosa Sprocati, Flavia Tasso, Salvatore Vacca, Giovanni De Giudici
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioremediation sulfate-reducing bacteria heavy metals biomineralization mine waters microbial diversity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2cddfe2ed0814449a3f9772e02bcdcf3
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spelling	oai:doaj.org-article:2cddfe2ed0814449a3f9772e02bcdcf32021-11-22T06:21:40ZMicrobial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters1664-302X10.3389/fmicb.2021.778199https://doaj.org/article/2cddfe2ed0814449a3f9772e02bcdcf32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.778199/fullhttps://doaj.org/toc/1664-302XIn order to increase the knowledge about geo-bio interactions in extreme metal-polluted mine waters, we combined microbiological, mineralogical, and geochemical analyses to study the indigenous sulfate-reducing bacteria (SRB) involved in the heavy metal (HM) biomineralization processes occurring in Iglesiente and Arburese districts (SW Sardinia, Italy). Anaerobic cultures from sediments of two different mining-affected streams of this regional framework were enriched and analyzed by 16S rRNA next-generation sequencing (NGS) technique, showing sequences closely related to SRB classified in taxa typical of environments with high concentrations of metals (Desulfovibrionaceae, Desulfosporosinus). Nevertheless, the most abundant genera found in our samples did not belong to the traditional SRB groups (i.e., Rahnella, Acinetobacter). The bio-precipitation process mediated by these selected cultures was assessed by anaerobic batch tests performed with polluted river water showing a dramatic (more than 97%) Zn decrease. Scanning electron microscopy (SEM) analysis revealed the occurrence of Zn sulfide with tubular morphology, suggesting a bacteria-mediated bio-precipitation. The inocula represent two distinct communities of microorganisms, each adapted to peculiar environmental conditions. However, both the communities were able to use pollutants in their metabolism and tolerating HMs by detoxification mechanisms. The Zn precipitation mediated by the different enriched cultures suggests that SRB inocula selected in this study have great potentialities for the development of biotechnological techniques to reduce contaminant dispersion and for metal recovery.Patrizia PaganinChiara AlisiElisabetta DoreDario FancelloPier Andrea MarrasDaniela MedasMaria Rita MonterealiStefano NaitzaNicola RigonatAnna Rosa SprocatiFlavia TassoSalvatore VaccaGiovanni De GiudiciFrontiers Media S.A.articlebioremediationsulfate-reducing bacteriaheavy metalsbiomineralizationmine watersmicrobial diversityMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioremediation sulfate-reducing bacteria heavy metals biomineralization mine waters microbial diversity Microbiology QR1-502
spellingShingle	bioremediation sulfate-reducing bacteria heavy metals biomineralization mine waters microbial diversity Microbiology QR1-502 Patrizia Paganin Chiara Alisi Elisabetta Dore Dario Fancello Pier Andrea Marras Daniela Medas Maria Rita Montereali Stefano Naitza Nicola Rigonat Anna Rosa Sprocati Flavia Tasso Salvatore Vacca Giovanni De Giudici Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
description	In order to increase the knowledge about geo-bio interactions in extreme metal-polluted mine waters, we combined microbiological, mineralogical, and geochemical analyses to study the indigenous sulfate-reducing bacteria (SRB) involved in the heavy metal (HM) biomineralization processes occurring in Iglesiente and Arburese districts (SW Sardinia, Italy). Anaerobic cultures from sediments of two different mining-affected streams of this regional framework were enriched and analyzed by 16S rRNA next-generation sequencing (NGS) technique, showing sequences closely related to SRB classified in taxa typical of environments with high concentrations of metals (Desulfovibrionaceae, Desulfosporosinus). Nevertheless, the most abundant genera found in our samples did not belong to the traditional SRB groups (i.e., Rahnella, Acinetobacter). The bio-precipitation process mediated by these selected cultures was assessed by anaerobic batch tests performed with polluted river water showing a dramatic (more than 97%) Zn decrease. Scanning electron microscopy (SEM) analysis revealed the occurrence of Zn sulfide with tubular morphology, suggesting a bacteria-mediated bio-precipitation. The inocula represent two distinct communities of microorganisms, each adapted to peculiar environmental conditions. However, both the communities were able to use pollutants in their metabolism and tolerating HMs by detoxification mechanisms. The Zn precipitation mediated by the different enriched cultures suggests that SRB inocula selected in this study have great potentialities for the development of biotechnological techniques to reduce contaminant dispersion and for metal recovery.
format	article
author	Patrizia Paganin Chiara Alisi Elisabetta Dore Dario Fancello Pier Andrea Marras Daniela Medas Maria Rita Montereali Stefano Naitza Nicola Rigonat Anna Rosa Sprocati Flavia Tasso Salvatore Vacca Giovanni De Giudici
author_facet	Patrizia Paganin Chiara Alisi Elisabetta Dore Dario Fancello Pier Andrea Marras Daniela Medas Maria Rita Montereali Stefano Naitza Nicola Rigonat Anna Rosa Sprocati Flavia Tasso Salvatore Vacca Giovanni De Giudici
author_sort	Patrizia Paganin
title	Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
title_short	Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
title_full	Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
title_fullStr	Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
title_full_unstemmed	Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters
title_sort	microbial diversity of bacteria involved in biomineralization processes in mine-impacted freshwaters
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2cddfe2ed0814449a3f9772e02bcdcf3
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Microbial Diversity of Bacteria Involved in Biomineralization Processes in Mine-Impacted Freshwaters

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