PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES

Anti-idiotypic antibodies (Ab2), according to the network theory of Jerne, are second-generation immunoglobulins that are produced against the idiotype of an antibody to a specific antigen. Despite the large number of works devoted to the study of the properties of these proteins, their role in the...

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Autores principales:	A. V. Trofimov, A. M. Ischenko, A. Ya. Rak, V. E. Sergeeva, A. S. Simbirtsev, N. B. Gamaleya, A. G. Berzina, N. V. Stankova, L. I. Ulyanova, T. A. Klimova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	antibodies anti-idiotypic antibodies elisa monoclonal antibodies morphine polyclonal antibodies Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2ce18c5632de483e9dbae1db0f7bf93a
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spelling	oai:doaj.org-article:2ce18c5632de483e9dbae1db0f7bf93a2021-11-18T08:03:48ZPREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES1563-06252313-741X10.15789/1563-0625-2019-5-987-996https://doaj.org/article/2ce18c5632de483e9dbae1db0f7bf93a2019-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1626https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAnti-idiotypic antibodies (Ab2), according to the network theory of Jerne, are second-generation immunoglobulins that are produced against the idiotype of an antibody to a specific antigen. Despite the large number of works devoted to the study of the properties of these proteins, their role in the regulation of the immune system is not fully known. It may consist in maintaining or blocking a minimal immune response to the antigen. The study of Ab2 is of great practical and scientific importance. The special properties of Ab2, namely, the ability to partially reproduce the structure of the primary antigen and, upon immunization, induce the appearance of tertiary antibodies, which, like first-generation antibodies, can bind to the antigen, have found application in the development of Ab2-based vaccines, in particular, for the treatment of tumors. In view of the presence of a number of limitations on research related to psychoactive substances, the development of Ab2- based vaccines against drug addiction also seems promising. To example, anti-idiotypic antibodies obtained for this purpose possessing a cocaine-like structure are described in the literature. In this work, murine monoclonal anti-idiotypic antibodies (mAb2) mimicking the structure of various morphine derivatives were obtained. Rabbit polyclonal antibodies to the 6-hemisuccinyl derivative of morphine conjugated with bovine serum albumin isolated by affinity chromatography were used as primary antibodies for immunization. Four hybridoma clones were obtained as a result of the fusion of immunized mice lymphocytes with mouse Sp2/0 mouse myeloma cells by the Milstein-Köhler method. After growth in animals, mAb2 produced by hybridoma cells were affinity purified. We investigated the physicochemical and antigenic properties of the isolated antibodies. It was shown that the obtained mAb2 differ in immunological specificity, competing in different degree with morphine derivatives for binding to first-generation antibodies. We tested the possibility of using the obtained mAb2 as antigen analogues in the solid-phase enzyme-linked immunosorbent assay to determine the titer of primary antibodies against morphine in the blood serum of laboratory animals immunized with morphine derivatives. Based on the obtained anti-idiotypic antibodies, it is proposed to develop test systems to determine the serum opiate-specific antibodies in people after specific vaccination for therapeutic or prophylactic purposes to avoid the use of drugs as antigens immobilized on the solid phase in the analysis.A. V. TrofimovA. M. IschenkoA. Ya. RakV. E. SergeevaA. S. SimbirtsevN. B. GamaleyaA. G. BerzinaN. V. StankovaL. I. UlyanovaT. A. KlimovaSPb RAACIarticleantibodiesanti-idiotypic antibodieselisamonoclonal antibodiesmorphinepolyclonal antibodiesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 5, Pp 987-996 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	antibodies anti-idiotypic antibodies elisa monoclonal antibodies morphine polyclonal antibodies Immunologic diseases. Allergy RC581-607
spellingShingle	antibodies anti-idiotypic antibodies elisa monoclonal antibodies morphine polyclonal antibodies Immunologic diseases. Allergy RC581-607 A. V. Trofimov A. M. Ischenko A. Ya. Rak V. E. Sergeeva A. S. Simbirtsev N. B. Gamaleya A. G. Berzina N. V. Stankova L. I. Ulyanova T. A. Klimova PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
description	Anti-idiotypic antibodies (Ab2), according to the network theory of Jerne, are second-generation immunoglobulins that are produced against the idiotype of an antibody to a specific antigen. Despite the large number of works devoted to the study of the properties of these proteins, their role in the regulation of the immune system is not fully known. It may consist in maintaining or blocking a minimal immune response to the antigen. The study of Ab2 is of great practical and scientific importance. The special properties of Ab2, namely, the ability to partially reproduce the structure of the primary antigen and, upon immunization, induce the appearance of tertiary antibodies, which, like first-generation antibodies, can bind to the antigen, have found application in the development of Ab2-based vaccines, in particular, for the treatment of tumors. In view of the presence of a number of limitations on research related to psychoactive substances, the development of Ab2- based vaccines against drug addiction also seems promising. To example, anti-idiotypic antibodies obtained for this purpose possessing a cocaine-like structure are described in the literature. In this work, murine monoclonal anti-idiotypic antibodies (mAb2) mimicking the structure of various morphine derivatives were obtained. Rabbit polyclonal antibodies to the 6-hemisuccinyl derivative of morphine conjugated with bovine serum albumin isolated by affinity chromatography were used as primary antibodies for immunization. Four hybridoma clones were obtained as a result of the fusion of immunized mice lymphocytes with mouse Sp2/0 mouse myeloma cells by the Milstein-Köhler method. After growth in animals, mAb2 produced by hybridoma cells were affinity purified. We investigated the physicochemical and antigenic properties of the isolated antibodies. It was shown that the obtained mAb2 differ in immunological specificity, competing in different degree with morphine derivatives for binding to first-generation antibodies. We tested the possibility of using the obtained mAb2 as antigen analogues in the solid-phase enzyme-linked immunosorbent assay to determine the titer of primary antibodies against morphine in the blood serum of laboratory animals immunized with morphine derivatives. Based on the obtained anti-idiotypic antibodies, it is proposed to develop test systems to determine the serum opiate-specific antibodies in people after specific vaccination for therapeutic or prophylactic purposes to avoid the use of drugs as antigens immobilized on the solid phase in the analysis.
format	article
author	A. V. Trofimov A. M. Ischenko A. Ya. Rak V. E. Sergeeva A. S. Simbirtsev N. B. Gamaleya A. G. Berzina N. V. Stankova L. I. Ulyanova T. A. Klimova
author_facet	A. V. Trofimov A. M. Ischenko A. Ya. Rak V. E. Sergeeva A. S. Simbirtsev N. B. Gamaleya A. G. Berzina N. V. Stankova L. I. Ulyanova T. A. Klimova
author_sort	A. V. Trofimov
title	PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
title_short	PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
title_full	PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
title_fullStr	PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
title_full_unstemmed	PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES
title_sort	preparation and properties of murine anti-idiotypic monoclonal antibodies recognizing primary rabbit polyclonal antibodies against morphine derivatives
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/2ce18c5632de483e9dbae1db0f7bf93a
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PREPARATION AND PROPERTIES OF MURINE ANTI-IDIOTYPIC MONOCLONAL ANTIBODIES RECOGNIZING PRIMARY RABBIT POLYCLONAL ANTIBODIES AGAINST MORPHINE DERIVATIVES

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