Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition

Recent reports have shown that bromelain (BL), a pineapple extract, acts as an adjuvant therapy in cancer treatment and prevention of carcinogenesis. The present study was designed to investigate the possible mechanisms by which BL could radiosensitize tumor cells in vitro and in a mouse tumor model...

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Autores principales:	Mai H. Mekkawy, Hanan A. Fahmy, Ahmed S. Nada, Ola S. Ali
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/2ce3ce92c4334e3da68de8f4241d03b4
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spelling	oai:doaj.org-article:2ce3ce92c4334e3da68de8f4241d03b42021-12-02T01:03:34ZRadiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition1534-73541552-695X10.1177/15347354211060369https://doaj.org/article/2ce3ce92c4334e3da68de8f4241d03b42021-11-01T00:00:00Zhttps://doi.org/10.1177/15347354211060369https://doaj.org/toc/1534-7354https://doaj.org/toc/1552-695XRecent reports have shown that bromelain (BL), a pineapple extract, acts as an adjuvant therapy in cancer treatment and prevention of carcinogenesis. The present study was designed to investigate the possible mechanisms by which BL could radiosensitize tumor cells in vitro and in a mouse tumor model. BL has shown a significant reduction in the viability of the radioresistant human breast carcinoma (MCF-7) cell line using cell proliferation assay. The in vivo study was designed using the Ehrlich model in female albino mice, treated with BL (6 mg/kg b. wt., intraperitoneal, once daily for 10 days) 1 hour before exposure to a fractionated dose of gamma radiation (5 Gy, 1 Gy for 5 subsequent days). The radiosensitizing effect of BL was evident in terms of a significant reduction in tumor volume, poly ADP ribose polymerase-1 (PARP-1), the proliferation marker Ki-67 and nuclear factor kappa activated B cells (NF-κB) with a significant elevation in the reactive oxygen species (ROS) content and lipid peroxidation (LPO) in tumor cells. The present findings offer a novel insight into the radiosensitizing effect of BL and its potential application in the radiotherapy course.Mai H. MekkawyHanan A. FahmyAhmed S. NadaOla S. AliSAGE PublishingarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENIntegrative Cancer Therapies, Vol 20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mai H. Mekkawy Hanan A. Fahmy Ahmed S. Nada Ola S. Ali Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
description	Recent reports have shown that bromelain (BL), a pineapple extract, acts as an adjuvant therapy in cancer treatment and prevention of carcinogenesis. The present study was designed to investigate the possible mechanisms by which BL could radiosensitize tumor cells in vitro and in a mouse tumor model. BL has shown a significant reduction in the viability of the radioresistant human breast carcinoma (MCF-7) cell line using cell proliferation assay. The in vivo study was designed using the Ehrlich model in female albino mice, treated with BL (6 mg/kg b. wt., intraperitoneal, once daily for 10 days) 1 hour before exposure to a fractionated dose of gamma radiation (5 Gy, 1 Gy for 5 subsequent days). The radiosensitizing effect of BL was evident in terms of a significant reduction in tumor volume, poly ADP ribose polymerase-1 (PARP-1), the proliferation marker Ki-67 and nuclear factor kappa activated B cells (NF-κB) with a significant elevation in the reactive oxygen species (ROS) content and lipid peroxidation (LPO) in tumor cells. The present findings offer a novel insight into the radiosensitizing effect of BL and its potential application in the radiotherapy course.
format	article
author	Mai H. Mekkawy Hanan A. Fahmy Ahmed S. Nada Ola S. Ali
author_facet	Mai H. Mekkawy Hanan A. Fahmy Ahmed S. Nada Ola S. Ali
author_sort	Mai H. Mekkawy
title	Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
title_short	Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
title_full	Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
title_fullStr	Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
title_full_unstemmed	Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition
title_sort	radiosensitizing effect of bromelain using tumor mice model via ki-67 and parp-1 inhibition
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/2ce3ce92c4334e3da68de8f4241d03b4
work_keys_str_mv	AT maihmekkawy radiosensitizingeffectofbromelainusingtumormicemodelviaki67andparp1inhibition AT hananafahmy radiosensitizingeffectofbromelainusingtumormicemodelviaki67andparp1inhibition AT ahmedsnada radiosensitizingeffectofbromelainusingtumormicemodelviaki67andparp1inhibition AT olasali radiosensitizingeffectofbromelainusingtumormicemodelviaki67andparp1inhibition
_version_	1718403305964568576

Radiosensitizing Effect of Bromelain Using Tumor Mice Model via Ki-67 and PARP-1 Inhibition

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