A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>

ABSTRACT Biofilms are resilient, surface-associated communities of cells with specialized properties (e.g., resistance to drugs and mechanical forces) that are distinct from those of suspension (planktonic) cultures. Biofilm formation by the opportunistic human fungal pathogen Candida albicans is me...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Clarissa J. Nobile, Emily P. Fox, Nairi Hartooni, Kaitlin F. Mitchell, Denes Hnisz, David R. Andes, Karl Kuchler, Alexander D. Johnson
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2ce4863501834480a71ccf81b7ac1787
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2ce4863501834480a71ccf81b7ac1787
record_format	dspace
spelling	oai:doaj.org-article:2ce4863501834480a71ccf81b7ac17872021-11-15T15:47:38ZA Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>10.1128/mBio.01201-142150-7511https://doaj.org/article/2ce4863501834480a71ccf81b7ac17872014-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01201-14https://doaj.org/toc/2150-7511ABSTRACT Biofilms are resilient, surface-associated communities of cells with specialized properties (e.g., resistance to drugs and mechanical forces) that are distinct from those of suspension (planktonic) cultures. Biofilm formation by the opportunistic human fungal pathogen Candida albicans is medically relevant because C. albicans infections are highly correlated with implanted medical devices, which provide efficient substrates for biofilm formation; moreover, biofilms are inherently resistant to antifungal drugs. Biofilms are also important for C. albicans to colonize diverse niches of the human host. Here, we describe four core members of a conserved histone deacetylase complex in C. albicans (Set3, Hos2, Snt1, and Sif2) and explore the effects of their mutation on biofilm formation. We find that these histone deacetylase complex members are needed for proper biofilm formation, including dispersal of cells from biofilms and multifactorial drug resistance. Our results underscore the importance of the physical properties of biofilms in contributing to drug resistance and dispersal and lay a foundation for new strategies to target biofilm dispersal as a potential antifungal intervention. IMPORTANCE Through the formation of biofilms—surface-associated communities of cells—microorganisms can establish infections, become drug resistant, and evade the host immune system. Here we investigate how four core members of a conserved histone deacetylase complex mediate biofilm formation by Candida albicans, the major fungal pathogen of humans. We show that this histone deacetylase complex is required for biofilm dispersal, a process through which cells leave the biofilm to establish new infections. We also show that the deacetylase complex mediates biofilm drug resistance. This work provides new insight into how the physical properties of biofilms affect dispersal and drug resistance and suggests new potential antifungal strategies that could be effective against biofilms.Clarissa J. NobileEmily P. FoxNairi HartooniKaitlin F. MitchellDenes HniszDavid R. AndesKarl KuchlerAlexander D. JohnsonAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 3 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Clarissa J. Nobile Emily P. Fox Nairi Hartooni Kaitlin F. Mitchell Denes Hnisz David R. Andes Karl Kuchler Alexander D. Johnson A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
description	ABSTRACT Biofilms are resilient, surface-associated communities of cells with specialized properties (e.g., resistance to drugs and mechanical forces) that are distinct from those of suspension (planktonic) cultures. Biofilm formation by the opportunistic human fungal pathogen Candida albicans is medically relevant because C. albicans infections are highly correlated with implanted medical devices, which provide efficient substrates for biofilm formation; moreover, biofilms are inherently resistant to antifungal drugs. Biofilms are also important for C. albicans to colonize diverse niches of the human host. Here, we describe four core members of a conserved histone deacetylase complex in C. albicans (Set3, Hos2, Snt1, and Sif2) and explore the effects of their mutation on biofilm formation. We find that these histone deacetylase complex members are needed for proper biofilm formation, including dispersal of cells from biofilms and multifactorial drug resistance. Our results underscore the importance of the physical properties of biofilms in contributing to drug resistance and dispersal and lay a foundation for new strategies to target biofilm dispersal as a potential antifungal intervention. IMPORTANCE Through the formation of biofilms—surface-associated communities of cells—microorganisms can establish infections, become drug resistant, and evade the host immune system. Here we investigate how four core members of a conserved histone deacetylase complex mediate biofilm formation by Candida albicans, the major fungal pathogen of humans. We show that this histone deacetylase complex is required for biofilm dispersal, a process through which cells leave the biofilm to establish new infections. We also show that the deacetylase complex mediates biofilm drug resistance. This work provides new insight into how the physical properties of biofilms affect dispersal and drug resistance and suggests new potential antifungal strategies that could be effective against biofilms.
format	article
author	Clarissa J. Nobile Emily P. Fox Nairi Hartooni Kaitlin F. Mitchell Denes Hnisz David R. Andes Karl Kuchler Alexander D. Johnson
author_facet	Clarissa J. Nobile Emily P. Fox Nairi Hartooni Kaitlin F. Mitchell Denes Hnisz David R. Andes Karl Kuchler Alexander D. Johnson
author_sort	Clarissa J. Nobile
title	A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
title_short	A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
title_full	A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
title_fullStr	A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
title_full_unstemmed	A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>
title_sort	histone deacetylase complex mediates biofilm dispersal and drug resistance in <named-content content-type="genus-species">candida albicans</named-content>
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/2ce4863501834480a71ccf81b7ac1787
work_keys_str_mv	AT clarissajnobile ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT emilypfox ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT nairihartooni ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT kaitlinfmitchell ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT deneshnisz ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT davidrandes ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT karlkuchler ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT alexanderdjohnson ahistonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT clarissajnobile histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT emilypfox histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT nairihartooni histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT kaitlinfmitchell histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT deneshnisz histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT davidrandes histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT karlkuchler histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent AT alexanderdjohnson histonedeacetylasecomplexmediatesbiofilmdispersalanddrugresistanceinnamedcontentcontenttypegenusspeciescandidaalbicansnamedcontent
_version_	1718427506832310272

A Histone Deacetylase Complex Mediates Biofilm Dispersal and Drug Resistance in <named-content content-type="genus-species">Candida albicans</named-content>

Ejemplares similares