Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation

Abstract Electronic cigarettes (e-cigarettes) are promoted as low-risk alternatives to combustible cigarettes. However, the effects of chronic inhalation of potential toxicants emitted by ecigarettes remain largely unexamined. It is conceivable that smoking-induced chronic diseases result in cellula...

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Autores principales:	A. Shaito, J. Saliba, A. Husari, M. El-Harakeh, H. Chhouri, Y. Hashem, A. Shihadeh, M. El-Sabban
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ce4cbc647da4836b86e1dd8641982ff
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spelling	oai:doaj.org-article:2ce4cbc647da4836b86e1dd8641982ff2021-12-02T11:52:23ZElectronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation10.1038/s41598-017-14634-z2045-2322https://doaj.org/article/2ce4cbc647da4836b86e1dd8641982ff2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14634-zhttps://doaj.org/toc/2045-2322Abstract Electronic cigarettes (e-cigarettes) are promoted as low-risk alternatives to combustible cigarettes. However, the effects of chronic inhalation of potential toxicants emitted by ecigarettes remain largely unexamined. It is conceivable that smoking-induced chronic diseases result in cellular injury, in the absence of effective repair by stem cells. This study evaluates the effect of cigarette and e-cigarette aerosol extracts on the survival and differentiation of bone marrow-derived mesenchymal stem cells (MSCs). MSC growth and osteogenic differentiation were examined after exposure to smoke extracts. Data revealed detrimental effects of both cigarette and e-cigarette extracts on MSC morphology and growth. Levels and activity of alkaline phosphatase, an osteogenic marker, decreased and induction of osteoblastic differentiation was impaired. Both smoke extracts prevented osteogenic differentiation from progressing, evident by decreased expression of terminal osteogenic markers and mineralization. Elevated levels of reactive oxygen species (ROS) were detected in cells exposed to smoke extracts. Moreover, decreased differentiation potential was concomitant with severe down-regulation of Connexin 43 expression, leading to the loss of gap junction-mediated communication, which together with elevated ROS levels, could explain decreased proliferation and loss of differentiation potential. Hence, e-cigarettes present similar risk as combustible cigarettes with respect to tissue repair impairment.A. ShaitoJ. SalibaA. HusariM. El-HarakehH. ChhouriY. HashemA. ShihadehM. El-SabbanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Shaito J. Saliba A. Husari M. El-Harakeh H. Chhouri Y. Hashem A. Shihadeh M. El-Sabban Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
description	Abstract Electronic cigarettes (e-cigarettes) are promoted as low-risk alternatives to combustible cigarettes. However, the effects of chronic inhalation of potential toxicants emitted by ecigarettes remain largely unexamined. It is conceivable that smoking-induced chronic diseases result in cellular injury, in the absence of effective repair by stem cells. This study evaluates the effect of cigarette and e-cigarette aerosol extracts on the survival and differentiation of bone marrow-derived mesenchymal stem cells (MSCs). MSC growth and osteogenic differentiation were examined after exposure to smoke extracts. Data revealed detrimental effects of both cigarette and e-cigarette extracts on MSC morphology and growth. Levels and activity of alkaline phosphatase, an osteogenic marker, decreased and induction of osteoblastic differentiation was impaired. Both smoke extracts prevented osteogenic differentiation from progressing, evident by decreased expression of terminal osteogenic markers and mineralization. Elevated levels of reactive oxygen species (ROS) were detected in cells exposed to smoke extracts. Moreover, decreased differentiation potential was concomitant with severe down-regulation of Connexin 43 expression, leading to the loss of gap junction-mediated communication, which together with elevated ROS levels, could explain decreased proliferation and loss of differentiation potential. Hence, e-cigarettes present similar risk as combustible cigarettes with respect to tissue repair impairment.
format	article
author	A. Shaito J. Saliba A. Husari M. El-Harakeh H. Chhouri Y. Hashem A. Shihadeh M. El-Sabban
author_facet	A. Shaito J. Saliba A. Husari M. El-Harakeh H. Chhouri Y. Hashem A. Shihadeh M. El-Sabban
author_sort	A. Shaito
title	Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
title_short	Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
title_full	Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
title_fullStr	Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
title_full_unstemmed	Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation
title_sort	electronic cigarette smoke impairs normal mesenchymal stem cell differentiation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2ce4cbc647da4836b86e1dd8641982ff
work_keys_str_mv	AT ashaito electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT jsaliba electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT ahusari electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT melharakeh electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT hchhouri electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT yhashem electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT ashihadeh electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation AT melsabban electroniccigarettesmokeimpairsnormalmesenchymalstemcelldifferentiation
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Electronic Cigarette Smoke Impairs Normal Mesenchymal Stem Cell Differentiation

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