Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis

Abstract Enzymatic catalysis in microreactors has attracted growing scientific interest because of high specific surface enabling heat and mass transfer and easier control of reaction parameters in microreactors. However, two major challenges that limit their application are fast inactivation and th...

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Autores principales:	An Gong, Chang-Tong Zhu, Yan Xu, Fang-Qin Wang, D’assise Kinfack Tsabing, Fu-An Wu, Jun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2ce9d3eb6f7e4ce99d4b71dad6e8f064
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spelling	oai:doaj.org-article:2ce9d3eb6f7e4ce99d4b71dad6e8f0642021-12-02T11:40:43ZMoving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis10.1038/s41598-017-04216-42045-2322https://doaj.org/article/2ce9d3eb6f7e4ce99d4b71dad6e8f0642017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04216-4https://doaj.org/toc/2045-2322Abstract Enzymatic catalysis in microreactors has attracted growing scientific interest because of high specific surface enabling heat and mass transfer and easier control of reaction parameters in microreactors. However, two major challenges that limit their application are fast inactivation and the inability to the biocatalysts in microchannel reactors. A fluid and unsinkable immobilized enzyme were firstly applied in a microchannel reactor for biocatalysis in this study. Functionalized forms of graphene-immobilized naringinase flowing in microchannels have yielded excellent results for isoquercitrin production. A maximum yield of 92.24 ± 3.26% was obtained after 20 min in a microchannel reactor. Ten cycles of enzymatic hydrolysis reaction were successively completed and an enzyme activity above 85.51 ± 2.76% was maintained. The kinetic parameter V m/K m increased to 1.9-fold and reaction time was decreased to 1/3 compared with that in a batch reactor. These results indicated that the moving and unsinkable graphene sheets immobilized enzyme with a high persistent specificity and a mild catalytic characteristic enabled the repetitive use of enzyme and significant cost saving for the application of enzyme catalysis. Thus, the developed method has provided an efficient and simple approach for the productive and repeatable microfluidic biocatalysis.An GongChang-Tong ZhuYan XuFang-Qin WangD’assise Kinfack TsabingFu-An WuJun WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q An Gong Chang-Tong Zhu Yan Xu Fang-Qin Wang D’assise Kinfack Tsabing Fu-An Wu Jun Wang Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
description	Abstract Enzymatic catalysis in microreactors has attracted growing scientific interest because of high specific surface enabling heat and mass transfer and easier control of reaction parameters in microreactors. However, two major challenges that limit their application are fast inactivation and the inability to the biocatalysts in microchannel reactors. A fluid and unsinkable immobilized enzyme were firstly applied in a microchannel reactor for biocatalysis in this study. Functionalized forms of graphene-immobilized naringinase flowing in microchannels have yielded excellent results for isoquercitrin production. A maximum yield of 92.24 ± 3.26% was obtained after 20 min in a microchannel reactor. Ten cycles of enzymatic hydrolysis reaction were successively completed and an enzyme activity above 85.51 ± 2.76% was maintained. The kinetic parameter V m/K m increased to 1.9-fold and reaction time was decreased to 1/3 compared with that in a batch reactor. These results indicated that the moving and unsinkable graphene sheets immobilized enzyme with a high persistent specificity and a mild catalytic characteristic enabled the repetitive use of enzyme and significant cost saving for the application of enzyme catalysis. Thus, the developed method has provided an efficient and simple approach for the productive and repeatable microfluidic biocatalysis.
format	article
author	An Gong Chang-Tong Zhu Yan Xu Fang-Qin Wang D’assise Kinfack Tsabing Fu-An Wu Jun Wang
author_facet	An Gong Chang-Tong Zhu Yan Xu Fang-Qin Wang D’assise Kinfack Tsabing Fu-An Wu Jun Wang
author_sort	An Gong
title	Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
title_short	Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
title_full	Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
title_fullStr	Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
title_full_unstemmed	Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
title_sort	moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2ce9d3eb6f7e4ce99d4b71dad6e8f064
work_keys_str_mv	AT angong movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT changtongzhu movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT yanxu movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT fangqinwang movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT dassisekinfacktsabing movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT fuanwu movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis AT junwang movingandunsinkablegraphenesheetsimmobilizedenzymeformicrofluidicbiocatalysis
_version_	1718395572296089600

Moving and unsinkable graphene sheets immobilized enzyme for microfluidic biocatalysis

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