Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis

Tieshan Yang,1 Qian Yao,1 Fei Cao,1 Qianqian Liu,1 Binlei Liu,2 Xiu-Hong Wang1 1Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, 2Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, Peopl...

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Autores principales:	Yang T, Yao Q, Cao F, Liu Q, Liu B, Wang XH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Silver nanoparticle (AgNPs) hypoxia-inducible factor transcriptional activity vascular endothelial growth factor-A angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2cecc69497034a47a189ddbe6c18307e
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spelling	oai:doaj.org-article:2cecc69497034a47a189ddbe6c18307e2021-12-02T00:09:56ZSilver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis1178-2013https://doaj.org/article/2cecc69497034a47a189ddbe6c18307e2016-12-01T00:00:00Zhttps://www.dovepress.com/silver-nanoparticles-inhibit-the-function-of-hypoxia-inducible-factor--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Tieshan Yang,1 Qian Yao,1 Fei Cao,1 Qianqian Liu,1 Binlei Liu,2 Xiu-Hong Wang1 1Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, 2Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that is activated upon exposure to hypoxic stress. It modulates a number of cellular responses including proliferation, apoptosis, angiogenesis, and metabolism by activating a panel of target genes in response to hypoxia. The HIF-1 level is often upregulated in the hypoxic microenvironment of solid tumors, which contributes to cancer treatment failure. Here we report that silver nanoparticles (AgNPs), which are widely used as an antimicrobial agent, are an effective inhibitor of HIF-1. AgNPs inhibited the activation of a HIF-dependent reporter construct after the cells were exposed to hypoxic conditions or treated with cobalt chloride, a hypoxia mimetic agent. The AgNPs also interfered with the accumulation of HIF-1α protein and the induction of the endogenous HIF target genes, VEGF-A and GLUT1. Since both HIF-1 and vascular endothelial growth factor-A play an important role in angiogenesis, AgNPs also inhibited angiogenesis in vitro. Our data reveal a new mechanism of how AgNPs act on cellular function, that is, they disrupt HIF signaling pathway. This finding provides a novel insight into how AgNPs can inhibit cancer cell growth and angiogenesis. Keywords: silver nanoparticles (AgNPs), hypoxia-inducible factor, transcriptional activity, vascular endothelial growth factor-A, angiogenesisYang TYao QCao FLiu QLiu BWang XHDove Medical PressarticleSilver nanoparticle (AgNPs)hypoxia-inducible factortranscriptional activityvascular endothelial growth factor-AangiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6679-6692 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticle (AgNPs) hypoxia-inducible factor transcriptional activity vascular endothelial growth factor-A angiogenesis Medicine (General) R5-920
spellingShingle	Silver nanoparticle (AgNPs) hypoxia-inducible factor transcriptional activity vascular endothelial growth factor-A angiogenesis Medicine (General) R5-920 Yang T Yao Q Cao F Liu Q Liu B Wang XH Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
description	Tieshan Yang,1 Qian Yao,1 Fei Cao,1 Qianqian Liu,1 Binlei Liu,2 Xiu-Hong Wang1 1Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, 2Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that is activated upon exposure to hypoxic stress. It modulates a number of cellular responses including proliferation, apoptosis, angiogenesis, and metabolism by activating a panel of target genes in response to hypoxia. The HIF-1 level is often upregulated in the hypoxic microenvironment of solid tumors, which contributes to cancer treatment failure. Here we report that silver nanoparticles (AgNPs), which are widely used as an antimicrobial agent, are an effective inhibitor of HIF-1. AgNPs inhibited the activation of a HIF-dependent reporter construct after the cells were exposed to hypoxic conditions or treated with cobalt chloride, a hypoxia mimetic agent. The AgNPs also interfered with the accumulation of HIF-1α protein and the induction of the endogenous HIF target genes, VEGF-A and GLUT1. Since both HIF-1 and vascular endothelial growth factor-A play an important role in angiogenesis, AgNPs also inhibited angiogenesis in vitro. Our data reveal a new mechanism of how AgNPs act on cellular function, that is, they disrupt HIF signaling pathway. This finding provides a novel insight into how AgNPs can inhibit cancer cell growth and angiogenesis. Keywords: silver nanoparticles (AgNPs), hypoxia-inducible factor, transcriptional activity, vascular endothelial growth factor-A, angiogenesis
format	article
author	Yang T Yao Q Cao F Liu Q Liu B Wang XH
author_facet	Yang T Yao Q Cao F Liu Q Liu B Wang XH
author_sort	Yang T
title	Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
title_short	Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
title_full	Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
title_fullStr	Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
title_full_unstemmed	Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
title_sort	silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/2cecc69497034a47a189ddbe6c18307e
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Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis

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