Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro

The glutathione system in the mitochondria of the brain plays an important role in maintaining the redox balance and thiol–disulfide homeostasis, whose violations are the important component of the biochemical shifts in neurodegenerative diseases. Mitochondrial dysfunction is known to be accompanied...

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Autores principales:	Dmitry S. Semenovich, Egor Yu. Plotnikov, Oksana V. Titko, Elena P. Lukiyenko, Nina P. Kanunnikova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	redox state glutathione system thiol–disulfide balance brain mitochondria oxidative stress D-panthenol Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2cf006805b264dda86a4388df68102c0
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spelling	oai:doaj.org-article:2cf006805b264dda86a4388df68102c02021-11-25T16:26:32ZEffects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro10.3390/antiox101116992076-3921https://doaj.org/article/2cf006805b264dda86a4388df68102c02021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1699https://doaj.org/toc/2076-3921The glutathione system in the mitochondria of the brain plays an important role in maintaining the redox balance and thiol–disulfide homeostasis, whose violations are the important component of the biochemical shifts in neurodegenerative diseases. Mitochondrial dysfunction is known to be accompanied by the activation of free radical processes, changes in energy metabolism, and is involved in the induction of apoptotic signals. The formation of disulfide bonds is a leading factor in the folding and maintenance of the three-dimensional conformation of many specific proteins that selectively accumulate in brain structures during neurodegenerative pathology. In this study, we estimated brain mitochondria redox status and functioning during induction of oxidative damage in vitro. We have shown that the development of oxidative stress in vitro is accompanied by inhibition of energy metabolism in the brain mitochondria, a shift in the redox potential of the glutathione system to the oxidized side, and activation of S-glutathionylation of proteins. Moreover, we studied the effects of pantothenic acid derivatives—precursors of coenzyme A (CoA), primarily D-panthenol, that exhibit high neuroprotective activity in experimental models of neurodegeneration. Panthenol contributes to the significant restoration of the activity of enzymes of mitochondrial energy metabolism, normalization of the redox potential of the glutathione system, and a decrease in the level of S-glutathionylated proteins in brain mitochondria. The addition of succinate and glutathione precursor N-acetylcysteine enhances the protective effects of the drug.Dmitry S. SemenovichEgor Yu. PlotnikovOksana V. TitkoElena P. LukiyenkoNina P. KanunnikovaMDPI AGarticleredox stateglutathione systemthiol–disulfide balancebrain mitochondriaoxidative stressD-panthenolTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1699, p 1699 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	redox state glutathione system thiol–disulfide balance brain mitochondria oxidative stress D-panthenol Therapeutics. Pharmacology RM1-950
spellingShingle	redox state glutathione system thiol–disulfide balance brain mitochondria oxidative stress D-panthenol Therapeutics. Pharmacology RM1-950 Dmitry S. Semenovich Egor Yu. Plotnikov Oksana V. Titko Elena P. Lukiyenko Nina P. Kanunnikova Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
description	The glutathione system in the mitochondria of the brain plays an important role in maintaining the redox balance and thiol–disulfide homeostasis, whose violations are the important component of the biochemical shifts in neurodegenerative diseases. Mitochondrial dysfunction is known to be accompanied by the activation of free radical processes, changes in energy metabolism, and is involved in the induction of apoptotic signals. The formation of disulfide bonds is a leading factor in the folding and maintenance of the three-dimensional conformation of many specific proteins that selectively accumulate in brain structures during neurodegenerative pathology. In this study, we estimated brain mitochondria redox status and functioning during induction of oxidative damage in vitro. We have shown that the development of oxidative stress in vitro is accompanied by inhibition of energy metabolism in the brain mitochondria, a shift in the redox potential of the glutathione system to the oxidized side, and activation of S-glutathionylation of proteins. Moreover, we studied the effects of pantothenic acid derivatives—precursors of coenzyme A (CoA), primarily D-panthenol, that exhibit high neuroprotective activity in experimental models of neurodegeneration. Panthenol contributes to the significant restoration of the activity of enzymes of mitochondrial energy metabolism, normalization of the redox potential of the glutathione system, and a decrease in the level of S-glutathionylated proteins in brain mitochondria. The addition of succinate and glutathione precursor N-acetylcysteine enhances the protective effects of the drug.
format	article
author	Dmitry S. Semenovich Egor Yu. Plotnikov Oksana V. Titko Elena P. Lukiyenko Nina P. Kanunnikova
author_facet	Dmitry S. Semenovich Egor Yu. Plotnikov Oksana V. Titko Elena P. Lukiyenko Nina P. Kanunnikova
author_sort	Dmitry S. Semenovich
title	Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
title_short	Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
title_full	Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
title_fullStr	Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
title_full_unstemmed	Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro
title_sort	effects of panthenol and n-acetylcysteine on changes in the redox state of brain mitochondria under oxidative stress in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2cf006805b264dda86a4388df68102c0
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Effects of Panthenol and N-Acetylcysteine on Changes in the Redox State of Brain Mitochondria under Oxidative Stress In Vitro

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