High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics

Abstract The ability to focus, separate and concentrate specific targets in a fluid is essential for the analysis of complex samples such as biological fluids, where a myriad of different particles may be present. Inertial focusing is a very promising technology for such tasks, and specially a recen...

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Autores principales:	Javier Cruz, Klas Hjort
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2cf17e71459b4d43a2a3edcb11261cb8
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spelling	oai:doaj.org-article:2cf17e71459b4d43a2a3edcb11261cb82021-12-02T18:33:58ZHigh-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics10.1038/s41598-021-93177-w2045-2322https://doaj.org/article/2cf17e71459b4d43a2a3edcb11261cb82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93177-whttps://doaj.org/toc/2045-2322Abstract The ability to focus, separate and concentrate specific targets in a fluid is essential for the analysis of complex samples such as biological fluids, where a myriad of different particles may be present. Inertial focusing is a very promising technology for such tasks, and specially a recently presented variant, inertial focusing in High Aspect Ratio Curved systems (HARC systems), where the systems are easily engineered and focus the targets together in a stable position over a wide range of particle sizes and flow rates. However, although convenient for laser interrogation and concentration, by focusing all particles together, HARC systems lose an essential feature of inertial focusing: the possibility of particle separation by size. Within this work, we report that HARC systems not only do have the capacity to separate particles but can do so with extremely high resolution, which we demonstrate for particles with a size difference down to 80 nm. In addition to the concept for particle separation, a model considering the main flow, the secondary flow and a simplified expression for the lift force in HARC microchannels was developed and proven accurate for the prediction of the performance of the systems. The concept was also demonstrated experimentally with three different sub-micron particles (0.79, 0.92 and 1.0 µm in diameter) in silicon-glass microchannels, where the resolution in the separation could be modulated by the radius of the channel. With the capacity to focus sub-micron particles and to separate them with high resolution, we believe that inertial focusing in HARC systems is a technology with the potential to facilitate the analysis of complex fluid samples containing bioparticles like bacteria, viruses or eukaryotic organelles.Javier CruzKlas HjortNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javier Cruz Klas Hjort High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
description	Abstract The ability to focus, separate and concentrate specific targets in a fluid is essential for the analysis of complex samples such as biological fluids, where a myriad of different particles may be present. Inertial focusing is a very promising technology for such tasks, and specially a recently presented variant, inertial focusing in High Aspect Ratio Curved systems (HARC systems), where the systems are easily engineered and focus the targets together in a stable position over a wide range of particle sizes and flow rates. However, although convenient for laser interrogation and concentration, by focusing all particles together, HARC systems lose an essential feature of inertial focusing: the possibility of particle separation by size. Within this work, we report that HARC systems not only do have the capacity to separate particles but can do so with extremely high resolution, which we demonstrate for particles with a size difference down to 80 nm. In addition to the concept for particle separation, a model considering the main flow, the secondary flow and a simplified expression for the lift force in HARC microchannels was developed and proven accurate for the prediction of the performance of the systems. The concept was also demonstrated experimentally with three different sub-micron particles (0.79, 0.92 and 1.0 µm in diameter) in silicon-glass microchannels, where the resolution in the separation could be modulated by the radius of the channel. With the capacity to focus sub-micron particles and to separate them with high resolution, we believe that inertial focusing in HARC systems is a technology with the potential to facilitate the analysis of complex fluid samples containing bioparticles like bacteria, viruses or eukaryotic organelles.
format	article
author	Javier Cruz Klas Hjort
author_facet	Javier Cruz Klas Hjort
author_sort	Javier Cruz
title	High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
title_short	High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
title_full	High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
title_fullStr	High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
title_full_unstemmed	High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
title_sort	high-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2cf17e71459b4d43a2a3edcb11261cb8
work_keys_str_mv	AT javiercruz highresolutionparticleseparationbyinertialfocusinginhighaspectratiocurvedmicrofluidics AT klashjort highresolutionparticleseparationbyinertialfocusinginhighaspectratiocurvedmicrofluidics
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High-resolution particle separation by inertial focusing in high aspect ratio curved microfluidics

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